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Journal of Biomedical Optics logoLink to Journal of Biomedical Optics
. 2021 Jun 2;26(6):069801. doi: 10.1117/1.JBO.26.6.069801

Single acquisition label-free histology-like imaging with dual-contrast photoacoustic remote sensing microscopy (Errata)

Benjamin Ecclestone a,b, Deepak Dinakaran b,c, Parsin Haji Reza a,*
PMCID: PMC8170389  PMID: 34080401

Abstract.

The errata correct the errors in citation numbering that appeared in the originally published article.


This article [J. Biomed. Opt. 26(5), 056007 (2021) doi: 10.1117/1.JBO.26.5.056007] was originally published on 25 May 2021 with erroneous citation numbering in the reference list and in the text.

In the reference list, the following references were corrected:

  • from 12 to 13: G. Stasi and E.M. Ruoti “A critical evaluation in the delivery of the ultrasound practice: the point of view of the radiologist,” Ital. J. Med. 9(1), 5 (2015)

  • from 13 to 12: T. T. W. Wong et al., “Fast label-free multilayered histology-like imaging of human breast cancer by photoacoustic microscopy,” Sci. Adv. 3(5), e1602168 (2017)

  • from 17 to 19: N. J. M. Haven et al., “Ultraviolet photoacoustic remote sensing microscopy,” Opt. Lett. 44, 3586–3589 (2019)

  • from 18 to 20: N. Haven et al., “Reflective objective-based ultraviolet photoacoustic remote sensing virtual histopathology,” Opt. Lett. 45, 535–538 (2020)

  • from 19 to 17: K. Bell et al., “Reflection-mode virtual histology using photoacoustic remote sensing microscopy,” Sci. Rep. 10, 19121 (2020)

  • from 20 to 18: B.R. Ecclestone et al., “Improving maximal safe brain tumor resection with photoacoustic remote sensing microscopy,” Sci. Rep. 10, 17211 (2020).

In the body of the text, the following citations were corrected:

  • from 12 to 13, for the sentence

    • “Acoustic transducers are typically bulky, have known interoperator technique reliability issues, and sometimes require immersion in a coupling media such as water to function.”

  • from 13 to 14, for the sentences

    • “PARS replaces the acoustically coupled ultrasound transducer with a detection laser.”

    • “Photoacoustic signals are then detected as pressure-induced modulations in the backscattered magnitude of the detection beam.”

    • “Observing backscattering in a reflection mode architecture allows PARS to image thick samples.”

  • from 14 and 15, to 15-18, for the sentence

    • “Moreover, PARS may provide chromophore-specific contrast by selecting excitation wavelengths to target unique biomolecule absorption spectra.”

  • from 19 and 20, to 17 and 18, for the sentences

    • “Previously, PARS has provided complete H&E emulation using a tunable excitation source to independently target the absorption peaks of DNA and cell membrane structures.”

    • “While effective in both thin sections and tissue blocks, this technique was largely limited in field of view, resolution, and imaging speed since it required the use of a slow (1 kHz) tunable excitation source.”

  • from 13 to 14, for the sentences

    • “In PARS systems, a cofocused pulsed excitation and continuous wave detection laser pair are used to capture photoacoustic absorption contrast.”

    • “The excitation induces photoacoustic signals by depositing focused pulses of optical energy into the sample.”

    • “The absorption contrast is then captured as nanosecond scale pressure-induced modulations in the backscattered intensity of the cofocused detection laser.”

    • “Usually, to capture MHz-scale PARS modulations, the time-resolved backscattering magnitude is band pass filtered to isolate the absorption signal.”

  • from 16 and 17, to 17 and 18 for the sentences

    • “Previous implementations developed by our group leverage a multiwavelength tunable excitation to capture hyperspectral images of several chromophores in the tissue.”

    • “In this implementation, we use a 50-kHz UVexcitation, which provides emulated H&E images substantially faster than the 1-kHz tunable source used in previous studies.”

The above-listed errors were corrected, and the article was republished on 26 May 2021.


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