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. 2021 May 13;11(14):7110–7125. doi: 10.7150/thno.61085

Figure 5.

Figure 5

Biodistribution of the liposomes after intratracheal injection. A: Western blot analysis of Srpx2, fibronectin, Col1a1 and α-SMA expression in the lungs of mice following BLM induction. Three mice were included in each study group. B: Results for co-immunostaining of Srpx2 and α-SMA in BLM-induced lung sections. C: Schematic diagram showing the preparation of Srpx2 siRNA-loaded liposomes. D: Representative IVIS images of a mouse at different time points after the administration of DiR-labeled liposomes. E: Ex vivo fluorescence images of major organs from mice. F: Immunofluorescence image showing the biodistribution of DiI-labeled liposomes (red) and Col1a1 (green) in the lungs of BLM-induced mice. The nuclei were stained blue by DAPI, and the images were taken at ×400 magnification. G: phagocytosis of SRPX2 siRNA-loaded liposomes in fibroblasts from IPF patients and control subjects. H-I: Western blot (H) and RT-PCR (I) analysis of the levels of temporal changes in Srpx2 expression in the lungs of transfected mice after BLM induction. Three mice were included in each study group. The data are represented as the mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001.