Table 2. Primer sequences to generate homology arms.
Forward primer sequence | Revers primer sequence | |
---|---|---|
5´HA no1 | gcatgcACGCGCTGTTAATCAAAT | gcggccgcACGCTGTAAGGAACACTGATG |
5´HA no2 | gcatgcCGCTGTCATCGCTGTAATTC | gcggccgcGTCCCGCGATCATTTTCC |
3´HA no1 | ggcgcgccTCAAGGCAAGGCCGTCC | ggtaccCGAATTAAATTTGGGCGTTT |
3´HA no2 | ggtaccCTTCGGTTGTTTCTGAACTTTATG | actagtTCTCCCACTCCCCAATTATG |