KEY RESOURCES TABLE.
REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Rabbit anti-Fos (Ab-5) | EMD Millipore | Cat# PC38; RRID:AB_2106755 |
Rabbit anti-NeuN | Novusbio | Cat# NBP1–77686SS; RRRID:AB_11027797 |
Donkey anti-rabbit IgG-Alexa Fluor 594 | Cellsignal | Cat# 8889; RRID:AB_2716249 |
Bacterial and Virus Strains | ||
AAV9.hSyn.eYFP.WPRE.bGH | James M. Wilson; unpublished | Addgene # 105539 |
AAV9.Syn.DIO.EGFP.WPRE.hGH | Ian Wickersham; unpublished | Addgene # 100043 |
AAV9.hSyn.hChR2.(H134R)-eYFP.WPRE.hGH | (Chan et al., 2017) | Addgene # 26973 |
AAV9.EF1a.DIO.hChR2(H134R)-eYFP.WPRE.hGH | (Chan et al., 2017) | Addgene # 20298 |
AAV9-EF1a-DIO-eYFP | (Chan et al., 2017) | Addgene # 27056 |
AAV9- EF1a-DIO-Arch- eYFP | (Chow et al., 2010) | Addgene # 22222 |
AAV8.Syn.DIO. mCherry | Bryan Roth; unpublished | Addgene # 50459 |
pAAV8-hSyn-DIO-hM4D(Gi)-mCherry | (Krashes et al., 2011) | Addgene # 44362 |
AAV8-Syn-DIO-HM3D(Gq)-mCherry | (Krashes et al., 2011) | Addgene # 44361 |
AAV9-Syn-DIO-GCaMP6s-WPRE-SV40 | (Chen et al., 2013) | Addgene # 100845 |
AAV9-Syn-GCaMP6s-WPRE-SV40 | (Chen et al., 2013) | Addgene # 100843 |
Experimental Models: Organisms/Strains | ||
Cck-IRES-Cre mice | Jackson Laboratories | Stock # 012706; RRID:IMSR_JAX:012706 |
C57BL/6J mice | Jackson Laboratories | Stock # 000664; RRID:IMSR_JAX:000664 |
SF1-cre mice | Jackson Laboratories | Stock # 012462; RRID:IMSR_JAX:012462 |
Software and Algorithms | ||
MATLAB | Mathworks | https://www.mathworks.com/ |
ImageJ | NIH | https://imagej.nih.gov/ij/index.html; RRID:SCR_003070 |
DeepLabCut | Mathis et al., 2018 | http://www.mousemotorlab.org/deeplabcut |
Miniscope Controller | UCLA Miniscope | https://github.com/daharoni/Miniscope_DAQ_Software |
Miniscope preprocessing/motion correction | Aharoni et al., 2019 | https://github.com/daharoni/Miniscope_Analysis |
Extraction of in vivo calcium signals | Zhou et al., 2018 | https://github.com/zhoupc/CNMF_E |
Other | ||
Orca Flash camera | Hamamatsu | https://www.hamamatsu.com/resources/pdf/sys/SCAS0081E_C11440-22CU.pdf |
Optogenetic Fiberoptic Cannulae | Doric Lenses | MFC_200/240–0.22_MF1.25_FLT |
Fiber photometry fiberoptic cannulae | Neurophotometrics | FOC_MF_400 |
Superfrost Plus slides | Fisher Scientific | Cat # 22–037-246 |
Microendoscope | UCLA Miniscope | http://miniscope.org/index.php/Main_Page |
Nanoinjector | World Precision Instruments | Cat# Nanoliter 2000 |