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. 2020 Oct 26;35(6):1696–1709. doi: 10.1038/s41375-020-01068-2

Fig. 4. Establishment of new SC lines from cultures.

Fig. 4

a After primary cultures, SCs from patients #5, #6 and #10 were plated between 42 and 63 days to achieve expansion in long-term culture. b Immunophenotype of the cell lines was evaluated before (original cells) and at the end point of the culture according to TCRVβ2 and CD3 expression to quantify tumor cells. Inside TCRVβ2 + CD3 + population, CCR7 and CD45RO expressions were used for assessing T-cell maturation stages (naive, TCM, TEM and TEMRA).