Fig. 7. Therapeutic response to HDAC inhibitors and doxorubicin on SC lines expansion in vitro.

1 × 10⁶ cells/well were plated and treated for 48 h with romidepsin (10 nM), doxorubicin (20 nM) and vorinostat (3 µM). Untreated and DMSO conditions were added as controls. a Example of Annexin V+/Hoechst-, Annexin V-/Hoechst+ and Annexin V+/Hoechst+ cell proportion analysis after treatments for L1. b Quantification of total death in each condition for 5 SC lines (mean ± SD). Data were analyzed using Mann–Whitney test and were considered statistically significant at *P < 0.05.