Fig. 4.

At2g32580 complements elmo1^−/−. (A) Ruthenium Red-stained dark-grown hypocotyls of elmo1^−/− or elmo1^−/− transformed with 35S:At2g3250. (B) Relative quantitation (RQ) using RT-qPCR of the indicated genotype, relative to actin. 35S:At2g32580 1 and 35S:At2g32580 2 indicate two independent elmo1^−/− 35S:At2g3250 transformants. Numbers above each bar show mean (top) and standard deviation (bottom). The elmo1 mutant has an almost 6-fold reduction (unpaired two tailed t-test, P<0.01) of At2g32580 expression relative to WT. At2g32580 levels in the two isolates of elmo1^−/− 35Sp:At2g32580 are not distinguishable from WT (unpaired two tailed t-test, P>0.01). Scale bar: 0.5 mm.