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[Preprint]. 2021 Jun 2:2021.05.31.21254851. [Version 1] doi: 10.1101/2021.05.31.21254851

Figure 4. Increased IL10RB expression is associated with worse COVID-19 outcomes in vivo and increased SARS-CoV-2 viral load in vitro.

Figure 4.

Panel A. Increased IL10RB expression is associated with worse COVID-19 outcomes in vivo. *, ** and *** for FDR-adjusted p≤0.05, 0.01 and 0.001 respectively Panel B. In vitro experimental overview. Panel C. CRISPRa gRNAs (IL10RB-1, IL10RB-2, IL10RB-3 and IL10RB-4) were used to overexpress IL10RB in hiPSC-derived NGN2-glutamatergic neurons. ***, ** and * correspond to p values from the linear model of equal or less than 0.001, 0.01 and 0.05 respectively. For the SARS-CoV-2 viral load (right panel) we perform pairwise comparison with unpaired t-test; ***, ** and * correspond to p values equal to, or less than, 0.001, 0.01 and 0.05 respectively. Panel D. Competitive gene set enrichment analysis in hiPSC-derived NGN-2 glutamatergic neurons. On the left side we are testing enrichment for canonical pathways and biological processes (Gene ontology) that are significantly enriched (FDR<0.05) in SARS-CoV-2 infection (top row) and on the right side for gene sets that correspond to betacoronavirus infections across different cell systems52 (only significant results are shown; FDR < 0.05).