Figure 3.

Female OIP5-AS1 mice demonstrated a worsened heart failure phenotype following TAC surgery

(A–G) (A) Schematic of experimental (transverse aortic constriction [TAC] or Sham surgery) protocol performed on male and female wild type (WT) and knock out (KO) mice, which were followed for a further 8 weeks before cull. Tissue weights from cull were normalized to tibia length for (B) whole heart weights, (C) left ventricle (LV), (D) right ventricle (RV), (E) atria, and (F) whole lung (♀ = female, ♂ = male, female n = 7–14/group, male n = 9–19, mean ± SEM, ∗p < 0.05 versus sham, #p < 0.05 versus WT-TAC of the same sex) (G) Percent fractional shortening (FS %) as determined by echocardiography in male and female WT and KO Sham and TAC mice at 8 weeks post-procedure (n = 3–5/group for sham, n = 6–11/group for TAC, mean ± SEM, ∗p < 0.05 versus sham for each sex.

(H) Representative photomicrographs of whole hearts from female and male WT and KO mice 8-weeks post procedure.

(I) Representative images of picrosirius red stained LV sections from female WT and KO mice post-TAC procedure, 400 x magnification, scale bar = 20m.

(J) Quantification of percent area picrosirius red staining (fibrosis) of entire LV section from WT and KO female mice undergoing TAC surgery (n = 6/group). For panels B-G and panel J, a non-parametric one-way ANOVA with multiple comparisons correction (Dunnet's) was used to test for significance, ∗p < 0.05.