Figure 1.

Building a gPhage library

(A) Phage is a self-replicating nanoparticle that combines genotype (DNA information) and phenotype (peptide), including the epitope of an IgG.

(B) T. cruzi genome is fragmented and cloned into the gPhage vector to produce a collection of phage particles, each displaying a T. cruzi-derived peptide.

(C–E) gPhage library quality control. (C) The plot shows the number of distinct reads observed in the gPhage library as a function of the total number of sequences evaluated after filtering. The plots indicate length of DNA inserts (D) and corresponding peptides (E) in the gPhage library. (F) Cartoon illustrating the odds of a random gDNA inserts yield a phage particle displaying a T. cruzi-derived peptide.

(G) Pie chart with gPhage library insert distribution: percentage of transformants containing a T. cruzi-derived insert (left circle); inserts that resulted in a peptide displayed on the surface of the bacteriophage particle (central circle), and finally, percentage of peptides that either match or do not match a T. cruzi protein in databanks.