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. 1987 Mar 1;28(1):47–54. doi: 10.1186/BF03548255

Virulence, Cytotoxic and Inflammatory Activities of Vibrio Anguillarum and Aeromonas Salmonicida Isolated from Cultivated Salmonid Fish in Sweden

Virulens, cytoxisk och inflammatorisk aktivitet hos Vibrio anguillarum och Aeromonas salmonicida isolerad från odlad laxfisk i Sverige

Karel Krovacek 1,✉, Ahmed Faris 1, Laila Eriksson 1, Eva Jansson 1, Olle Ljungberg 1, Ingmar Månsson 1
PMCID: PMC8185786  PMID: 3687646

Abstract

Extracellular products in culture filtrates of Aeromonas salmonicida subsp. achromogenes and Vibrio anguillarum isolated from infected fish have been shown to possess skin inflammatory factor. The extracellular products from Vibrio anguillarum were cytotoxic in HeLa and CHO cells. In addition to the skin lesions, the culture filtrates of V. anguillarum caused necrotic reaction on the rabbit skin. Five of 6 strains of V. anguillarum were lethal to mice after intraperitoneal administration of 3×107 CFU. Only 1 strain of 5 A. salmonicida subsp. achromogenes produced extracellular products which elicited cytotoxic effects in the CHO cells. None of the A. salmonicida subsp. achromogenes strains were lethal to mice. The cytotoxins were inactivated when heated at 65°C for 30 min. The results indicate that the thermolabile exotoxins are non-enterotoxic since they failed to stimulate fluid accumulation in the rabbit ileal loop and did not cause elongation of the CHO cells. The rounding off of CHO cells, as well as of HeLa cells indicate that the exotoxins may play an important role in fish diseases.

Keywords: Aeromonas salmonicida, Vibrio anguillarum, bacterial toxins, fish diseases, virulence factors.

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Acknowledgements

We wish to express our sincere gratitude to Birgitta Evemar and Rakel Axelsson for the preparation of the manuscript.

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