Author Correction: Gasdermin D restricts Burkholderia cenocepacia infection in vitro and in vivo

Correction to: Scientific Reports 10.1038/s41598-020-79201-5, published online 13 January 2021

The original version of this Article contained an error in Figure 6B where were the y-axis numbering “0, 10, 20, 30” was incorrectly given as “0, 1, 2, 3”.

The original Figure 6 and accompanying legend appear below.

Figure 6.

Gsdmd^−/− mice and their macrophages exhibit less autophagosme formation during B. cenocepacia infection. (A) Confocal images of LC3 (green) immunofluorescence assay of B. cenocepacia (B.c.) (red) (MOI10) infected WT and gsdmd^−/− macrophages at 6 h post-infection. White arrows points to B. cenocepacia- LC3 co-localization. Scale bar: 10 µM. (B) Quantification of B. cenocepacia co-localized with LC3 in B. cenocepacia infected WT and gsdmd^−/− macrophages treated as in (A). Data represent mean ± SEM (n = 5). Statistical analysis was performed using two-way ANOVA. (C) Quantification of total LC3 puncta in B. cenocepacia infected WT and gsdmd^−/− macrophages treated as in (A) using ImageJ Software. Values are mean percentage ± SEM calculated by scoring 96 randomly chosen fields of view from 6 independent experiments. Statistical analysis was performed using paired two tailed Student’s t-test. (D) Immunoblot analysis of LC3 in whole cell lysate separated of WT and gsdmd^−/− macrophages treated as in (A). (E) Immunoblot analysis of LC3 in lung homogenate separated of WT and gsdmd^−/− mice after being infected intratracheally for 4 h by B. cenocepacia (10 × 10⁶ CFU/mouse) (n = 5). (F) Densitometry analysis of immunoblots represented in (E). Data represent mean ± SEM (n = 5). Statistical analysis was performed using unpaired two-tailed student’s t-test *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.

The original Article has been corrected.