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. 2021 May 24;24(6):102643. doi: 10.1016/j.isci.2021.102643

Figure 2.

Figure 2

Identification of FliI as a potential molecular target involved in OA process

(A) Gene enrichment analysis for functional categories of top-regulated genes in murine chondrocytes cocultured with murine macrophages stimulated by cartilage fragments.

(B) Violin plots showing the fold change of top-regulated genes in chondrocytes.

(C) Expression of FliI in chondrocytes as analyzed by qRT-PCR. Results represent means ± SEM for triplicates and significant difference was determined by the student t-test.

(D) Detection of FliI in OA cartilage using IHC. Scale bars are indicated and represent 500 μm for low magnification image and 100 μm for higher magnification images.

(E) Detection of FliI in chondrocytes isolated from OA cartilage using IFA. Scale bars represent 50 μm.

(F) Detection of FliI in synovial fluids by ELISA (left panel) and Western blotting (right panel).

(G) Detection of FliI in supernatant of chondrocyte cultures stimulated with IL-1β or TNF-α using ELISA (upper panel) and Western blotting (lower panel). Results represent means ± SEM for triplicates and significant difference was determined by the One-way ANOVA, followed by Tukey's multiple-comparison procedure.