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. 2021 May 5;24(6):102511. doi: 10.1016/j.isci.2021.102511
REAGENT OR RESOURCE SOURCE IDENTIFIER
Bacterial strains

Lactobacillus rhamnosus GG ATCC Cat# 53103
Lactobacillus acidophilus ATCC Cat# 4356
Lactobacillus bulgaricus LB-87 Custom Probiotic Inc Cat# CP-2016
Lactobacillus casei LC-11 Custom Probiotic Inc Cat# CP-2012
Lactobacillus salivarius LS-33 Custom Probiotic Inc Cat# CP-2010
Bacteroides thetaiotaomicron ATCC Cat# 29148
Bacteroides fragilis ATCC Cat# 25285
Eubacterium rectale ATCC Cat# 33656
E. coli Top10 Invitrogen Cat# C404006
E. coli BL21 (DE3) Invitrogen Cat# C600003

Plasmids and primers

pET28b-MBP-TEV Addgene Cat# 69929
pET28b-MBP-TEV-Msp1 This study N/A
pET28b-MBP-TEV-Msp2 This study N/A
pET28b-MBP-TEV-Msp3 This study N/A
Primers see Table S5 N/A N/A

Chemicals, Peptides, and Recombinant Proteins

MRS broth Hardy Diagnostics Cat# C5931
BHI broth Hardy Diagnostics Cat# C5141
Lemon fruits Sam’s Club Cat#127308
Kanamycin Sigma Cat# K1876
RNAprotect Bacteria Reagent Qiagen Cat# 76506
Porcine bile extract Sigma Cat# B8631
Ni-NTA Agarose Qiagen Cat# 30210
IRDye 800cw streptavidin VWR Cat# 102673-342
Dynabeads™ M-270 Streptavidin Invitrogen Cat# 65305
Tskgel G5000PW column VWR Cat# 100368-036
SuperScrip III First-Strand Synthesis System Invitrogen Cat#18080051
RNase-free DNase І NEB Cat# M0303S
TEV protease Sigma Cat# T4455
Nuclease P1 NEB Cat# M0660S
CIP NEB Cat# M0525S

Critical Commercial Assays

RNeasy Mini Kit Qiagen Cat# 74104
miRNeasy Mini Kit Qiagen Cat# 217004
RiboPure RNA Purification Kit, bacteria Invitrogen Cat# AM1925
QuantiTect SYBR Green PCR Kits Qiagen Cat# 204143
PKH26 Fluorescent Cell Linker Kit Sigma Cat# PKH26GL
GeneArt Seamless Cloning and Assembly Kit Invitrogen cat# A14606
HiScribe T7 Quick High Yield RNA Synthesis Kit NEB Cat# E2050S
QIAamp DNA Stool Mini Kits Qiagen Cat# 51504

Experimental Models: Organisms/Strains Mouse

C57BL/6 Jackson Laboratory Stock Cat# 000664

Deposited data

Sequenced data reported in this paper is stored at Sequence Read Archive (SRA): Accession code for Deposited Data: PRJNA722680

Software and algorithms

Prism (GraphPad Software) GraphPad https://www.graphpad.com
BioEdit BioEdit https://www.bioedit.com
Sequence manipulation suite (Stothard, 2000) https://www.bioinformatics.org/sms2