Abstract
Ninety E. coli strains, isolated from piglets which had died from neonatal diarrhea, were tested for the presence of K88, K99, 987P and type 1 fimbriae. Two or more types of fimbriae were demonstrated in 14 of the strains, a single fimbria! type in 44 strains while in 32 strains no fimbriae were detected. Of the 14 E. coli strains with more than 1 type of fimbriae, 12;, 10, 8 and 4 strains showed K88, K99, 987P and type 1, respectively.
Twelve E. coli strains were isolated from piglets which had died in the neonatal period without showing signs of neonatal diarrhea at necropsy. One strain showed 987P and 3 strains showed type 1 fimbriae, while the remaining 8 strains were unfimbriated.
Sixteen fimbriated E. coli strains were subcultured in order to examine the stability of fimbrial expression in the strains. The K88 and the type 1 fimbriae were regularly expressed, while the K99 and 987P were inconsistently demonstrated.
Keywords: pili, K-antigens, adhesins, neonatal diarrhea
Sammendrag
Nitti E. coli stammer isolert fra 55 spedgriser døde av neonatal diare, er undersøkt med hensyn på fimbrier (K88, K99, 987P og type 1). Fjorten av stammene hadde mer enn 1 fimbrie, 44 stammer hadde en fimbrie hver og 32 stammer ble funnet å være uten fimbrier. Av de 14 E. coli stammene med mer enn 1 fimbrie, hadde, 12, 10, 8, og 4 stammer henholdsvis K88, K99, 987P og type 1.
Tolv E. coli stammer isolert fra spedgriser døde av andre årsaker enn neonatal diare ble inkludert. En av disse stammene hadde 987P, 3 hadde type 1 mens de (resterende 8 stammene var uten fimbrier.
Seksten E. coli stammer med fimbrier ble subkultivert for å undersøke reproduserbarheten i påvisningen av fimbriene. K88 og type 1 fimbriene ble ofte gjenfunnet, mens K99 og 987P ikke kunne reproduseres i samme grad.
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Acknowledgments
The excellent technical assistance of Tove Nilsen is highly appreciated.
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