NMJ transmission defects correlate with motor unit connectivity (a–c). Jitter showed significant inverse correlation with CMAP amplitude (Pearson r=−0.7022, p=0.0024), nonsignificant direct correlation with SMUP (Pearson r=0.4134, p=0.1114), and significant indirect correlation with MUNE (Pearson r=−0.5507, p=0.0271). d–f Similarly, blocking showed significant inverse correlation with CMAP amplitude (Spearman rs = −0.7760, p=0.0008), nonsignificant direct correlation with SMUP (Spearman rs = 0.4051, p= 0.1200), and significant indirect correlation with MUNE (Spearman rs = −0.6289, p=0.0110). g–i RNS showed no significant correlation with CMAP amplitude (Pearson r=3896, p=0.1357) but significant indirect correlation with SMUP (Pearson r=−0.5241, p=0.0372) and significant direct correlation with MUNE (Pearson r=0.6284, p=0.0091). Young rats, n=8 (3 females, 5 males), and aged rats, n=8 (3 females, 5 males)