COVID-19 in the Context of Inborn Errors of Immunity: a Case Series of 31 Patients from Mexico

Abstract

Introduction

Patients with inborn errors of immunity (IEI) have a compromised or inappropriate immune response. Although they might be considered a high-risk group for severe SARS-CoV-2 infection, the reported impact of COVID-19 in these patients has been reassuring, while the differential susceptibility of distinct types of IEI remains unclear.

Objective

We aimed to describe the findings and outcomes of our known patients with IEI who were diagnosed with COVID-19.

Methods

In a retrospective study from March 2020 to February 2021, four centers in Mexico collected clinical, laboratory, and genetic data from pediatric and adult patients with known diagnoses of IEI who presented with COVID-19, based on compatible symptoms and positive SARS-CoV-2 testing or known household exposure.

Results

We report 31 patients with known IEI from Mexico who presented with SARS-CoV-2 infection. Seventy-four percent were male, 52% were pediatric, and 81% survived. Their ages ranged from 5 months to 56 years, with a median of 17 years. Sixty-five percent had predominant antibody deficiencies, 48% were hospitalized, and 26% required ICU. Pediatric patients had a higher hospital admission rate than adults. Inpatient mortality was 40%, and ICU mortality rate was 63%. Forty-eight percent developed pneumonia, while 36% had evidence of hyperinflammation (4 adults and 7 children). Predominant laboratory features were lymphopenia and thrombocytopenia, seen in 70 and 44% of patients, respectively. The serum D-dimer median value was 2.6 (0.5–20.6) μg/mL, and the median highest ferritin value was 1015 (32–10,303) ng/mL. Intravenous immunoglobulin was used in 80% of patients. Other treatments included macrolides (39%) and corticosteroids (29%). Six patients died from secondary infection or uncontrolled systemic inflammation.

Discussion

Although impaired immunity due to IEI may be a predisposing factor for severe COVID-19, most of our patients with IEI who acquired the SARS-CoV-2 infection developed a well-tolerated infection and survived, as have more than 80% of worldwide reported patients to date. An impaired immune or inflammatory response may be a predisposing factor for some and a protective factor for others. A systematic review of the literature could help identify those patients at risk of severe disease and complications. Healthcare-associated infections should be aggressively prevented.

Introduction

The current SARS-CoV-2 pandemic has caused over 2.6 million deaths after more than 117 million people were infected worldwide. In Mexico, more than 2.1 million confirmed cases have left over 190,000 deaths after 12 months [1]. The novel betacoronavirus zoonotic spillover spread swiftly around the world in a few months, causing asymptomatic or mild disease in about 80% of patients. Differential susceptibility became an early concern, especially among patients, clinicians, and researchers living with, treating, or studying, immune deficiencies.

The main risk factors for severe SARS-CoV-2 infection, inflammatory complications, and death are old age, obesity, and male sex [2, 3]. Pre-existing diseases related to “inflammaging,” such as diabetes mellitus, hypertension, and chronic heart or lung disease, also predict a poor outcome [3, 4]. Strikingly, a considerable number of patients without any known risk factor have also developed critical respiratory failure, neurologic or systemic inflammatory complications, and death. An overzealous inflammatory response to the coronavirus causes more damage than the viral infection per se.

In at least 14% of patients with life-threatening COVID-19, autoantibodies against type 1 interferons, or pathogenic variants in genes involved in innate immune sensing or interferon viral response, have been identified [5, 6]. Increased IL-1β, IL-6, and TNF-$\alpha$ serum concentrations, and NLRP3 inflammasome activation, have been found and are known to play a role in the pathophysiology of hospitalized patients with acute respiratory distress and neurologic or systemic inflammatory response (“brain fog,” “cytokine storm,” “Kawasaki-like,” or multisystem inflammatory syndrome) [5, 7, 8].

Inborn errors of immunity (IEI) are a group of more than 450 rare congenital diseases with a wide clinical spectrum of increased susceptibility to infections, inflammation, atopy, autoimmunity, and/or cancer [9]. The study of patients with COVID-19 in the context of known IEI, with varying immune component deficiencies, may further inform our understanding of the virus-host interactions and help dissect our immune system response to the virus.

Objective

We aimed to describe the findings and outcomes of our known patients with IEI who were diagnosed with COVID-19.

Methods

In a multicenter retrospective study, we collected clinical, laboratory, and genetic data from patients with known diagnoses of IEI who presented with SARS-CoV-2 infection during the first 12 months of the pandemic in Mexico. Two centers in Mexico City (one for adults, one for pediatric patients), one in Monterrey, and one in Chihuahua, contributed with information.

Previously diagnosed patients (in accordance with current diagnostic guidelines) were included by their clinicians, immunologists at referral centers for IEI. SARS-CoV-2 infection was diagnosed when patients with compatible symptoms had a positive real-time reverse transcription-polymerase chain reaction (RT-PCR) test, a positive rapid colorimetric assay, a positive antibody test during or after the acute disease, and/or, patients who were close contacts of confirmed COVID-19 cases (household family members).

IBM SPSS Statistics for Windows, Version 26.0 (Armonk, NY) was used for statistical analysis. Qualitative data were reported as frequencies and percentages. Quantitative data were described as median, minimum, and maximum values, assuming a non-normal data distribution given the small number and heterogeneity of the patients. Nonparametric Mann–Whitney U-test was used to compare quantitative data, and Fisher's exact test was used to analyze categorical variables. A p-value < 0.05 was considered statistically significant.

Results

Thirty-one patients with COVID-19 in the context of an IEI were identified and followed from March 2020 to February 2021, at four centers in Mexico. Most patients (74%) were male, and almost two-thirds (20 patients, 65%) had predominantly antibody deficiencies, group III in the International Union of Immunological Societies classification [9]. Five patients had phagocyte defects, three a combined immunodeficiency (two syndromic), one a disease of immune dysregulation, one an autoinflammatory disorder, and one a phenocopy of a primary immune deficiency (Good syndrome). Sixteen patients (52%) had confirmed genetic diagnoses. Their ages ranged from 5 months to 56 years, with a median of 17 years. Sixteen (52%) of the patients in our series were pediatric. Three of the patients (P2, P3, P25) had been previously included in an international report of COVID-19 in IEI [10].

Overall, 87% of the patients had preexisting comorbidities. In 58% there was a history of chronic lung disease, bronchiectasis, asthma, or fungal or mycobacterial lung infection. In 23% there was history of autoimmune disease, mainly cytopenias. When explored, however, differences in hospitalization, ICU admission, or outcome were not statistically significant.

For SARS-CoV-2 confirmation, multiple methods were employed. Real-time reverse transcription-polymerase chain reaction (RT-PCR) was performed in 28 patients, 24 of whom had a positive result (86%). Of the 4 patients with negative RT-PCR, three were diagnosed based on close contact with confirmed cases and compatible symptoms, and one case was diagnosed through specific IgM for SARS-CoV-2. Of the remaining cases, one was diagnosed through a positive rapid antigen test, one based on close contact and symptoms, and one case by positive IgG for SARS-CoV-2. Specific IgG tests for SARS-CoV-2 were performed in 5 patients, and only 2 were positive; (one had Chronic granulomatous disease and one an Autoinflammatory disorder). Three patients had a negative IgG despite having previous positive RT-PCR results; these patients had either combined (ARPC1B, CD3D) or predominantly antibody defects (SAD).

Almost half of the patients (48%) were hospitalized, eight required intensive care (26%), and six died (19%). Fifteen of 31 patients developed COVID-19 pneumonia (48%), and 11 of 31 (36%) had evidence of hyperinflammation or MIS-C (4 adults and 7 children). Table 1 and 2 summarize the patients’ clinical and laboratory characteristics.Table 3 highlights the most relevant results, and Table 4 compares hospital and ICU admittance in children and adults.

Table 1.

Patients’ characteristics

Patient ID/Center	Sex	Age at COVID-19 infection (years)	IUIS IEI classification category	Specific diagnosis	Genetic defect	Comorbidities	Clinical manifestations and involved organs	Complications	Required hospital admission	Required ICU admission	Medications used	Outcome
P1/CHIH	Male	0.45	I Combined	SCID	CD3D	GERD	Diarrhea, hypoxemia. Pneumonia	Myocarditis	Yes	No	IVIG (2 g/kg)	Alive, awaiting HSCT
P2/INP	Male	0.8	II Combined Syndromic	ARPC1B deficiency	ARPC1B	None	Fever, septic shock, hypoxemia Pneumonia	Pseudomonas aeruginosa bacteremia	Yes	No	IVIG (1 g/kg)	Alive
P3/INP	Male	3	II Combined Syndromic	WAS	WAS LOF	Post HSCT Inflammatory bowel disease Multiple food allergies Allergic reaction to IVIG CMV infection. Chronic lung disease	Fever, cough, hypoxemia. Pneumonia	CMV infection progression, compromising CNS, lung, and bone marrow	Yes	Yes	SCIG (4 g/kg)	Dead
P4/INP	Male	15	III Antibody	CVID	N/A	AIHA Recurrent infections Bronchiectasis	Fever, cough, hypoxemia. Pneumonia. Hypotension, cardiovascular alterations Abdominal pain and diarrhea	ARDS. Hyperinflammation Thrombocytopenia Neutropenia Splenic fungal balls Recurrent Campylobacter bacteremia	Yes	Yes	IVIG (2 g/kg) Tocilizumab (8 mg/kg for 3 doses), Methylprednisolone 1 mg/kg, Ivermectin (200 mcg/kg 1 dose) CsA (2 mg/kg)	Alive
P5/INP	Male	15	III Antibody	CVID	N/A	Epilepsy	Headache, fever, hypoxemia. Pneumonia Abdominal pain and diarrhea	None	Yes	No	IVIG (2 g/kg)	Alive
P6/INP	Female	9	III Antibody	CVID	TNFSF13 (APRIL)	Recurrent infections. Chronic lung disease	Fever, hypoxemia, hypotension. Pneumonia, cardiovascular alterations	Thrombocytopenia Low cardiac ejection fraction and dilation of the left coronary artery Facial herpes zoster	Yes	Yes	IVIG (2 g/kg), Methylprednisolone 1 mg/kg	Alive
P7/INP	Male	13	III Antibody	XLA	BTK	Recurrent infections	URI, cough, arthralgias, fever	Pneumonia. Secondary hemophagocytic syndrome. Pulmonary hemorrhage	Yes	Yes	IVIG (4 g/kg), Methylprednisolone, cyclosporine, etoposide	Dead
P8/IMSS MTY	Male	22	III Antibody	XLA	BTK	Chronic rhinosinusitis	URI	None	Yes	No	IVIG (1 g/kg)	Alive
P9/IMSS MTY	Male	52	III Antibody	CVID	N/A	Chronic Diarrhea	URI, abdominal pain, diarrhea	Bronchiectasis. Nodular lymphoid hyperplasia	No	No	IVIG (1 g/kg)	Alive
P10/CMN SXXI	Female	19	III Antibody	IgG subclass deficiency	N/A	Asthma, chronic rhinosinusitis	Fever, fatigue, URI	None	No	No	IVIG (600 mg/kg), clarithromycin	Alive
P11/CMN SXXI	Female	40	III Antibody	CVID	N/A	ITP, chronic diarrhea, hypothyroidism	URI	None	No	No	IVIG (600 mg/kg), clarithromycin	Alive
P12/CMN SXXI	Male	26	III Antibody	CVID	N/A	Evans Syndrome, splenomegaly, antiphospholipid syndrome, morbid obesity, BQ	Fatigue, arthralgia, myalgia, headache, cough, dyspnea	Pseudomonas aeruginosa bacteremia. Aspergillus fumigatus infection	Yes	Yes	IVIG (1 gr/kg), dexamethasone, clarithromycin	Alive
P13/CMN SXXI	Female	51	III Antibody	CVID	N/A	Chronic diarrhea, BQ, chronic rhinosinusitis	Fatigue, fever, arthralgia, myalgia, headache, cough, dyspnea	Pulmonary fibrosis	No	No	IVIG (600 mg/kg), clarithromycin, levofloxacin	Alive
P14/CMN SXXI	Male	28	III Antibody	CVID	N/A	ITP, splenomegaly, chronic rhinosinusitis	Fatigue, headache, myalgias	None	No	No	SCIG, clarithromycin	Alive
P15/CMN SXXI	Female	31	III Antibody	CVID	N/A	ITP, BQ, chronic diarrhea, hypothyroidism	Fatigue, fever, arthralgia, myalgia, headache, cough, dyspnea	None	No	No	SCIG, clarithromycin, dexamethasone	Alive
P16/CMN SXXI	Male	23	III Antibody	XLA	BTK	BQ, chronic rhinosinusitis	Fatigue, fever, arthralgia, myalgia, headache, cough	None	No	No	SCIG, clarithromycin	Alive
P17/CMN SXXI	Female	24	III Antibody	CVID	N/A	BQ, ITP, chronic rhinosinusitis	Fatigue, fever, arthralgia, myalgia, headache, cough, dyspnea	None	No	No	SCIG, clarithromycin, dexamethasone	Alive
P18/CMN SXXI	Male	21	III Antibody	XLA	BTK	BQ, pulmonary fibrosis, pulmonary hypertension, chronic rhinosinusitis	Fatigue, fever, arthralgia, myalgia, headache, cough, dyspnea	None	No	No	SCIG, clarithromycin	Alive
P19/CMN SXXI	Male	38	III Antibody	XLA	BTK	BQ, chronic rhinosinusitis	Fatigue, fever, arthralgia, myalgia, headache, cough, dyspnea	Pseudomonas aeruginosa and Acinetobacter baumannii bacteremia	Yes	Yes	IVIG (1 gr/kg), dexamethasone, clarithromycin	Dead
P20/CMN SXXI	Male	56	III Antibody	CVID	N/A	BQ, chronic rhinosinusitis	Fatigue, fever, arthralgia, myalgia, headache, cough, dyspnea	None	No	No	IVIG (600 mg/kg), clarithromycin	Alive
P21/IMSS MTY	Male	13	III Antibody	XLA	BTK	None	Headache, malaise, vomit, rash, fever	None	No	No	None	Alive
P22/IMSS MTY	Male	17	III Antibody	XLA	BTK	None	URI, fever, malaise, myalgias, arthralgias	None	No	No	None	Alive
P23/IMSS MTY	Female	10	III Antibody	SAD	N/A	Lung Coccidioidomycosis	Fever, myalgias, headache, nausea/vomit, stomachache	None	No	No	None	Alive
P24/INP	Male	1.9	IV Dysregulation	Underway	Underway	AIHA, hepatosplenomegaly, lymphadenopathies, chronic diarrhea, eczema, suspected cow milk allergy CMV infection	Fever, paleness, and fatigue Cyanosis	Exacerbated autoimmune hemolytic anemia. Mild pericardial effusion and left coronary artery dilation	Yes	No	IGIV (2 g/kg), Methylprednisolone 30 mg/kg. Prednisone 2 mg/kg	Alive
P25/INP	Male	54	V Phagocyte	CGD gp91phox	CYBB	Recurrent infections, chronic lung disease	Anosmia	None	No	No	None	Alive
P26/INP	Male	16	V Phagocyte	CGD gp91phox	CYBB	Recurrent infections, chronic osteomyelitis	Fever, tachycardia, hypoxemia, shock	Pneumonia Hypotension, septic and cardiogenic shock, myocarditis, third grade AV block Renal and hepatic failure Thrombocytopenia, disseminated intravascular coagulation	Yes	Yes	IVIG (1.7 g/kg), Methylprednisolone 1 mg/kg	Dead
P27/INP	Female	8	V Phagocyte	CGD p67phox	NFC2	Previous HLH associated with Salmonella infection and suspected lung aspergillosis	URI	None	No	No	None	Alive
P28/INP	Male	11	V Phagocyte	CGD gp91phox	CYBB	Recurrent infections	Upper respiratory symptoms	None	No	No	None	Alive
P29/INP	Male	16	V Phagocyte	CGD gp91phox	CYBB	Recurrent infections, chronic lung Aspergillus infection with thoracic wall abscess	Upper respiratory symptoms, fever, cough	None	Yes	No	IVIG (2 g/kg)	Alive
P30/IMSS MTY	Male	12	VII Autoinflammatory	N/A	N/A	N/A	Fever, fatigue, seizure, arthralgias, arthritis, myalgia, cough, headache	Vasculitis	Yes	No	IVIG	Dead
P31/CMN SXXI	Male	51	X Phenocopy	Good Syndrome	N/A	ITP, tinea corporis, BQ	Fatigue, fever, arthralgia, myalgia, headache, cough, dyspnea	Pseudomonas aeruginosa and Acinetobacter baumannii bacteremia, hemothorax	Yes	Yes	IVIG (1 gr/kg), dexamethasone, clarithromycin	Dead

Abbreviations: GERD gastroesophageal reflux disease, IVIG intravenous immunoglobulin, HSCT hematopoietic stem cell transplant, WAS Wiskott-Aldrich syndrome, CMV cytomegalovirus, CNS central nervous system, SCIG subcutaneous immunoglobulin, AIHA autoimmune hemolytic anemia, CVID common variable immunodeficiency, CsA cyclosporin A, URI upper respiratory infection, CGD chronic granulomatous disease, HLH hemophagocytic lymphohistiocytosis, XLA X-linked agammaglobulinemia, ITP immune thrombocytopenia, BQ bronchiectasis, SAD specific antibody deficiency, P2, P3, and P25 were previously reported in (8)

Table 2.

Patients’ findings

Patient ID/center	RT-PCR for SARS-CoV-2	IgG for SARS-CoV-2	COVID-19 pneumonia	Evidence of hyperinflammation/cytokine storm	Highest sedimentation rate (mm/h)/C reactive protein (mg/dL)	Lowest lymphocyte count (Cells/μL)	Lowest platelet count (Platelets/μL)	Highest d dimer determination (µg/mL)	Highest ferritin concentration (ng/mL)	Highest pro BNP (pg/mL)
P1/CHIH	Negative	IgG negative, IgM positive taken in the acute phase	Yes	Yes	N/A/8.2	1400	360,000	0.7	145	343
P2/INP	Positive	Negative	Yes	No	N/A	1100	107,000	N/A	268	N/A
P3/INP	Positive	N/A	Yes	Yes	31/41	0	25,000	0.9	2524	N/A
P4/INP	Negative*	N/A	Yes	Yes	60/20	300	21,000	0.6	1995	N/A
P5/INP	Negative*	N/A	Yes	No	N/A	1000	214,000	N/A	N/A	N/A
P6/INP	Negative*	N/A	Yes	Yes	N/A/7	1086	284,000	20.1	283	N/A
P7/INP	Positive	N/A	Yes	Yes	72/19.6	0	20,000	14.0	2657	718
P8/IMSS	Positive	N/A	Yes	Yes	52/26.6	700	229,000	3.8	10,303	N/A
P9/IMSS	Positive	N/A	No	No	N/A/0.13	N/A	200,000	3.4	N/A	N/A
P10/CMN SXXI	Positive	N/A	No	No	N/A	3110	335,000	N/A	N/A	N/A
P11/CMN SXXI	Positive	N/A	No	No	N/A	1810	173,000	N/A	N/A	N/A
P12/CMN SXXI	Positive	N/A	Yes	Yes	N/A	440	45,000	0.54	611	N/A
P13/CMN SXXI	Positive	N/A	Yes	No	N/A	1090	149,000	N/A	N/A	N/A
P14/CMN SXXI	Positive	N/A	No	No	N/A	1090	366,000	N/A	N/A	N/A
P15/CMN SXXI	Positive	N/A	Yes	No	N/A	1370	124,000	N/A	N/A	N/A
P16/CMN SXXI	Positive	N/A	No	No	N/A	1700	350,000	N/A	N/A	N/A
P17/CMN SXXI	Positive	N/A	Yes	No	N/A	860	146,000	N/A	N/A	N/A
P18/CMN SXXI	Positive	N/A	Yes	No	N/A	3250	31,000	N/A	N/A	N/A
P19/CMN SXXI	Positive	N/A	Yes	Yes	N/A	330	174,000	5.78	2836	N/A
P20/CMN SXXI	Positive	N/A	Yes	No	N/A	1780	140,000	N/A	N/A	N/A
P21/IMSS MTY	N/A*	N/A	No	No	N/A	N/A	N/A	N/A	N/A	N/A
P22/IMSS MTY	N/A+	N/A	No	No	31/1.7	782	194,000	N/A	N/A	N/A
P23/IMSS MTY	Positive	Negative	No	No	N/A	N/A	N/A	N/A	N/A	N/A
P24/INP	Positive	N/A	No	No	N/A	4140	329,000	1.8	459	N/A
P25/INP	N/A	Positive	No	No	N/A	N/A	N/A	N/A	N/A	N/A
P26/INP	Positive	N/A	Yes	Yes	24/14	300	76,000	20.6	6464	4.319
P27/INP	Positive	N/A	No	No	N/A	1900	246,000	N/A	32	21
P28/INP	Positive	N/A	No	No	N/A	N/A	N/A	N/A	N/A	N/A
P29/INP	Positive	N/A	No	No	33/8	1300	238,000	N/A	N/A	N/A
P30/IMSS MTY	Positive	Positive	N/A	Yes	55/2.66	300	152,000	0.655	459	N/A
P31/CMN SXXI	Positive	N/A	Yes	Yes	N/A	50	138,000	2.60	1420	N/A

N/A not available

^*Patient was diagnosed given close contact with a confirmed case and clinical manifestations

⁺Patient was diagnosed with a rapid antigen test

P2, P3, and P25 were previously reported in⁸

Table 3.

Summary of patients’ characteristics

Age (Median, range)	17 years (0.5–56)
Male to female ratio	2.9:1
Hospital admission	15/31 (48%)
Combined immunodeficiencies (n = 3)	3/3 (100%)
Antibody defects (n = 20)	7/20 (35%)
Phagocytic defects (n = 5)	2/5 (40%)
ICU admission	8/31 (26%)
Combined immunodeficiencies (n = 3)	1/3 (33%)
Antibody defects (n = 20)	5/20 (25%)
Phagocytic defects (n = 5)	1/5 (20%)
Deaths	6/31 (19%)
Combined immunodeficiencies (n = 3)	1/3 (33%)
Antibody defects (n = 20)	2/20 (10%)
Phagocytic defects (n = 5)	1/5 (20%)
Hyperinflammation	11/31 (36%)
Combined immunodeficiencies (n = 3)	2/3 (67%)
Antibody defects (n = 20)	6/20 (30%)
Phagocytic defects (n = 5)	1/5 (20%)
Pneumonia	17/31 (55%)
Combined immunodeficiencies (n = 3)	3/3 (100%)
Antibody defects (n = 20)	12/20 (60%)
Phagocytic defects (n = 5)	1/5 (20%)

^*Detailed data is available for combined immunodeficiencies, antibody, and phagocytic defects, since they were the most frequently encountered. Dysregulation disorders, autoinflammatory, and phenocopies were not included since only one patient from each was available and information can be found in Tables 1 and 2

Table 4.

Hospital and ICU admission and mortality in children and adults

	Children	Adults	Fisher’s exact test	Odds ratio (95%CI)
Hospital admission	11/16 (69%)	4/15 (26%)	p = 0.032	6.1 (1.3–28.7)
ICU admission	5/16 (31%)	3/15 (20%)	p = 0.685	1.8 (0.4–9.5)
Mortality	4/16 (25%)	2/15 (13%)	p = 0.654	2.2 (0.3–14.1)

Of the 15 patients who required hospital admission 6 died, for an inpatient mortality of 40%, while 5 out of the 8 patients who required intensive care died (63%). When comparing patients with XLA and CVID (7 versus 11), no significant differences were found, neither in hospital or ICU admission, nor in mortality. The six patients that died had evidence of hyperinflammation; 2 out of the 4 pediatrics deaths were secondary to MIS-C. Mortality in patients with hyperinflammation was 55%. Hyperinflammation was associated with a fatal outcome, with an OR of 2.2 (95% CI 1.2–4.2), p = 0.001.

The following is a short depiction of the deceased patients: P3, a 3-year-old male with WAS post-HSCT who died due to uncontrolled CMV infection; P7, a 13-year-old male with XLA who died of pulmonary bleeding in the context of secondary hemophagocytic syndrome; P19, a 38-year-old male with XLA who died from a healthcare-associated infection; P26, a 16-year-old male with CGD who died from uncontrolled MIS-C; P30, a 12-year-old male with an autoinflammatory syndrome who died from rapidly progressive MIS-C; and P31, a 51-year-old male with Good syndrome who died from a healthcare-associated infection.

Acute-phase reactants are rarely assessed in ambulatory patients. Only in 8 patients was the erythrocyte sedimentation rate (ESR) measured, 7 of whom were hospitalized; the median ESR was 43 (24–72) mm per hour (Reference values < 15–20 mm/h in patients under 50 years and < 20–30 mm/h in patients over 50 years[11]), and there was no significant difference in patients who required hospital admission. Eleven patients had C-reactive protein (CRP) values measured (Reference value < 0.8 mg/dL [12]), 9 hospitalized and 2 ambulatory patients. We found higher values in patients who required hospital admission (14.0 vs 0.9 mg/dL, Mann–Whitney U test p = 0.036).

Complete blood counts (CBC) for lymphocyte and platelet counts were available in 26 patients, 11 ambulatory and 15 hospitalized. Lowest lymphocyte counts had a median of 1088 (0–4,140) cells/uL. There was a lower median lymphocyte count in hospitalized patients (440 vs 1700 cells/uL, Mann–Whitney U test p = 0.004). Sixty-one percent of the patients had lymphopenia below 1500 cells/uL, and this finding was associated with higher odds of hospital admission (Fisher’s exact test p = 0.021, OR 16.8 95%CI 1.6–176.2).

The median lowest platelet count was 152,000 (20,000–366,000) platelets/uL (Reference values 150,000–350,000). While thrombocytopenia was seen frequently in 12 out of 27 patients (44%), it was usually not severe; only 5 patients had thrombocytopenia below 50,000 platelets/uL. The D-dimer value was available in 13 patients, only 1 ambulatory; median was 2.6 (0.5–20.6) μg/mL (Reference values 0.22–0.50 μg/mL). We found no difference in D-dimer values in patients requiring ICU admission.

Ferritin concentration was available in 14 patients, all hospitalized; the median highest ferritin value was 1015 (32–10,303) ng/mL (Reference values 24–336 ng/mL in males and 11–307 ng/ml in females). Hyperferritinemia over 500 ng/dL was associated with ICU admission (Fisher’s exact test p = 0.026, OR 35 95%CI 1.7–703.0).

We had six deaths in our series: 4 children and 2 adults. Three of the pediatric patients died due to uncontrolled hyperinflammation unleashed by SARS-CoV-2, and one pediatric patient died from uncontrolled cytomegalovirus (CMV) infection. The two adult patients died from healthcare-associated infections from the same bacteria species (see Table 1).

For COVID-19 treatment, over 80% received treatment with immunoglobulin, either intravenous or subcutaneous. The most frequently used medications after immunoglobulin were macrolides (39%) and corticosteroids (29%). Two patients received cyclosporine A as part of an immunomodulatory regimen (P4 and P7). Only one patient received treatment with tocilizumab and ivermectin (P4). One patient (P30) was under chronic treatment with anakinra for his IEI.

Four cases of interest: catastrophic hyperinflammation and a SCID patient.

Patient 7 was a patient with XLA (BTK) and chronic lung disease due to recurrent respiratory infections. He coursed with SARS-CoV-2 pneumonia that unleashed a secondary hemophagocytic syndrome, requiring treatment with IVIG, high-dose corticosteroids, cyclosporine A, and etoposide. The patient remained with pancytopenia despite treatment and ultimately perished due to pulmonary hemorrhage.

Patient 26 was an adolescent male with X-linked chronic granulomatous disease (CYBB) and recurrent infections, chronic vertebrae osteomyelitis, and suspected active lung tuberculosis, who presented with fever and rapidly progressed to cardiogenic shock, myocarditis, third grade auriculoventricular block, and multiorgan failure. He fulfilled MIS-C criteria with high-grade fever, elevated acute phase reactants, and severe multiorgan disease, including cardiac, renal, hepatic, lung, and hematologic compromise. He also had highly elevated acute-phase reactants, lymphopenia, thrombocytopenia, hyperferritinemia, and elevated NT-proBNP. Several family members were positive for SARS-CoV-2, as was the patient. Despite treatment with corticosteroids, IVIG and multiple vasoactive medications, pump failure continued, and the patient died.

Patient 30 was a 12-year-old male with an autoinflammatory disorder (recurrent fever, arthralgias, and high acute phase reactants, without a conclusive genetic diagnosis after whole-exome sequencing), who was chronically treated with anakinra, steroid, and IVIG. He had a catastrophic disease over 48 h, starting with arthralgia and stupor, then rapidly developing fever and systemic inflammation, ultimately causing his demise.

Patient 1 was a 5-month-old male infant of Mennonite descent from Northern Mexico, whose sister had died with pneumonia and candidiasis. A female cousin was diagnosed with SCID due to CD3d deficiency. He was admitted with respiratory distress and acrocyanosis for suspected COVID-19 pneumonia, from which his mother was recovering. A chest CT scan revealed atypical pneumonia. The respiratory virus panel, including SARS-CoV-2, came back negative, but an antibody test taken during his second hospital day was IgM positive (IgG negative). Immunological workup reported pan-hypogammaglobulinemia and low CD3 + (CD4 + , CD8 +) T cell counts. He had elevated serum CRP, D-dimer, CK-MB and NT-proBNP, with normal troponin. Liver function tests reported AST 52 UI/L, ALT 22 UI/L, bilirubin 1.88 mg/dL, albumin 3.2 g/dL, and procalcitonin 0.20 ng/mL. His ferritin serum levels were 145 ng/mL, IL-6: 1.40 pg/mL, and LDH 555 UI/L. Mild viral myocarditis was diagnosed based on elevated cardiac markers; the echocardiographic evaluation showed normal biventricular function, and the patient did not require aminergic support but was started on digoxin. He responded well to oxygen support, antibiotics, and IVIG at 2 g/kg and was discharged home after days. Targeted exome sequencing for 407 genes identified a homozygous nonsense variant (p.Arg68Ter) in CD3D. The patient is currently alive and well, awaiting hematopoietic stem cell transplantation.

Discussion

We describe the findings and outcomes of 31 patients with COVID-19 in the context of different IEI during the first 12 months of the SARS-CoV-2 pandemic in Mexico. Of the patients 74% were male, 65% had antibody deficiencies, 48% were hospitalized, and 81% survived.

Ambulatory patients rarely have an extensive laboratory work-up taken, and some laboratory values should be cautiously interpreted as predictors. We did find higher CRP values in patients who required hospital admission and hyperferritinemia over 500 ng/mL more frequently in patients who required ICU admittance.

The limitations of this study include its retrospective nature and a relatively small sample. It is, however, the largest report from our country, with adult and pediatric patients from four centers in three cities.

Around the world, there are currently reports of over 330 patients with known IEI who suffered from SARS-CoV-2 infection. More than 80% have survived [10, 13–16]. Three patients from this series, patients 2, 3 and 25, had been previously described in an international collaborative report [10]. Some case series have found a minor impact with a mild course among these patients [14], while others warn about higher rates of complications, care needs, and inpatient mortality rate [15]. In our series, pediatric patients with IEI had a higher hospital admission rate than adults. However, we found no difference in the frequency of pneumonia or hyperinflammation response. ICU admission and mortality were not significantly different in pediatric patients.

In patients with antibody defects, there have been reports suggesting that there is an inverse relationship between the severity of primary antibody deficiencies and the severity of SARS-CoV-2 infection, as some patients with agammaglobulinemia and absent B cells had a milder course when compared with patients with CVID, who have dysfunctional but present B cells [14]. This intrinsic lack of B cells might contribute to an attenuated inflammatory reaction to SARS-CoV-2[17]. Several patients who recovered from COVID-19 lacked neutralizing antibodies[18, 19]. The relatively good outcome in patients with predominantly antibody defects would ratify the importance of cellular immunity against SARS-CoV-2. Nevertheless, it is not a simple matter, and we have not found a “rule of thumb” that stands without notable exceptions. No group of IEI seems to be immune or doomed to develop inflammatory complications.

Based on what we know about the pathophysiology of COVID-19 and the risk factors for severe disease, most of the damage is caused by an excessive, inappropriate immune response to the viral infection, not by the virus itself. Older age, obesity, and male sex are the strongest risk factors for severity and death in the general population. Besides immunosenescence and “inflammaging,” any pro-inflammatory condition or immunosuppression could negatively affect the host response and outcome during SARS-CoV-2 infection [3, 4, 15]. Viral-induced type II and IV hypersensitivity reactions have been described in association with SARS-CoV-2. An overtly active immune response in COVID-19 might unbridle a disproportionately high inflammatory response [19, 20].

When investigating patients with life-threatening COVID-19; however, only autoantibodies against type 1 interferons and monogenic defects that involve viral sensing or interferon response have been identified, as opposed to a wider range of immune deficiencies [5, 6]. This holds true also for rheumatic conditions. The novel betacoronavirus infection does not seem to be more frequent or severe in patients with known systemic autoimmune diseases (not even in those who received rituximab) [21, 22], in HIV-coinfection under antiretroviral treatment, or in primary immunodeficient patients [10, 23, 24].

Patients with inflammasome-mediated autoinflammatory disorders deserve special consideration, as the NLRP3 inflammasome has been implicated in the development of uncontrolled systemic inflammatory response [25, 26]. Those patients followed for cryopyrin-associated periodic syndrome, or any patients with inflammasome dysregulation, should probably take extreme preventive measures to avoid exposure.

SARS-CoV-2 RT-PCR detects viral RNA; and while a positive result is highly specific for the presence of the virus, the sensitivity is not well determined and can be affected by the timing of testing, sample collection, and laboratory techniques [27]. In our series, 75% had a positive result of the RT-PCR test. Out of the seven negative patients, two were confirmed through specific antibodies, one with a rapid colorimetric test, and the other four were considered infected given their caregivers’ positive results and the patients’ symptoms. Thus, a negative RT-PCR does not exclude COVID-19 infection.

When analyzing the cause of death of our patients, we noticed that none were directly attributable to the virus. Two adult patients died from healthcare-associated infections from the same bacteria species (Pseudomonas aeruginosa and Acinetobacter baumannii). It is important to bear in mind that patients with IEI might have a higher susceptibility to nosocomial infections [15], and when hospitalized for any cause, protective measures should be kept in place, as immunocompromised patients might have higher rates of morbidity and mortality from secondary bacterial infections.

Some patients with COVID-19 might develop a hyperinflammation phenotype, which might contribute to morbidity and mortality[28]. Hyperinflammation associated with SARS-CoV-2 infection was seen in 11 patients, 4 of them adults. A subset of patients with COVID-19 and hyperinflammation may present with a multisystemic inflammatory syndrome (MIS), with symptoms overlapping with Kawasaki disease and frequently showing signs of cardiac dysfunction [29–31]. MIS-C was originally described in pediatric patients, but a similar multisystem inflammatory syndrome in adults (MIS-A) has been described with edematous myocarditis, frequently requiring intensive care and aminergic support [32–34]. Both adult patients who died in our series had evidence of hyperinflammation, but none had evidence of MIS-A.

Three of the pediatric patients perished from uncontrolled hyperinflammation, two from MIS-C and one from secondary hemophagocytic lymphohistiocytosis (HLH). Patient 30 had an autoinflammatory disorder, P26 had CGD, and P7 had XLA. Underlying IEI might have predisposed them to undergo a “perfect (cytokine) storm,” despite timely treatment with IVIG [35]. HLH has been recognized as an important complication of CGD, and COVID-19 may be a trigger [36, 37]. Meyts et al. reported one CGD patient with COVID-19 complicated with HLH associated to Burkholderia, with three other CGD patients presenting a mild disease [10]. Our deceased patient with CGD (P26) presented a clinical picture compatible with MIS-C refractory to IVIG and corticosteroids.

SARS-CoV-2 infection might worsen the residual immune function of patients living with IEI. Patient 3 had a severe systemic reactivation of CMV, progressive and refractory, which was triggered after COVID-19, despite having had several negative viral loads before; this ultimately caused his death. We also noted that patients with IEI and those who undergo transplantation might have a prolonged SARS-CoV-2 excretion over 6–8 weeks, as has been seen with other viral respiratory infections [38, 39].

SARS-CoV-2 infection can also trigger autoimmunity in predisposed patients [40–42]; in patient 24, the infection seems to have unleashed an exacerbation of autoimmune hemolytic anemia in a patient who had been controlled with immunomodulatory treatment. Interestingly, this patient did not present any respiratory manifestations of COVID-19.

Finally, we might be observing a protective effect of pre-existing immunological conditions in patients who seek medical attention for COVID-19. Healthcare providers are more likely to be aware of their IEI patients’ susceptibility of infections and risk of non-infectious complications, more prone to admit to a hospital under close supervision, to start invasive procedures and second-line treatments, and to ask for help. A “VIP” treatment of sorts, for our most vulnerable patients.

Over 80% of our patients received IVIG as treatment during the acute infection. Human IVIG preparations might have antibodies derived from common cold coronaviruses, which could cross-react with SARS-CoV-2 and have an immunomodulatory effect [43, 44]. The timing of treatment with immune suppressants is crucial: dexamethasone, tocilizumab, or anakinra should only be started when the patient is admitted for COVID-19 pneumonia or if there is evidence of hyperinflammation. Immune modulation may be considered to blunt the excessive immune response induced by the virus [20, 45–49]. In contrast, interferon treatment may be a suitable therapeutic option early when the patient becomes symptomatic [50, 51]. Early type I interferon administration might also be beneficial in selected patients with IEI or auto antibodies impairing type I interferon response [52, 53].

In the future, we would like to collect and compare more information from Mexican patients and reports from around the world, to confirm associations and further explore potential risk and protective factors.

In conclusion, most of our known patients with IEI who acquired the SARS-CoV-2 infection developed a well-tolerated infection and survived. Continued, early, and high-dose treatment with IVIG might be beneficial in this group of patients. Pediatric patients with IEI may have a higher need for hospitalization. An impaired immune response due to IEI may be a predisposing factor for some and, paradoxically, a protective factor for others. COVID-19 may be the first in a series of unfortunate events that lead to a patient’s demise, either due to hyperinflammation or deterioration of residual immune function; health-care-associated infections should be aggressively prevented.

Abbreviations

CGD

Chronic granulomatous disease

CMV

Cytomegalovirus

COVID-19

Coronavirus disease 2019

CVID

Common-variable immune deficiency

HSTC

Hematopoietic stem cell transplantation

IBM

International Business Machines Corporation

IVIG

Intravenous immunoglobulin

MIS-C

Multisystem inflammatory syndrome in children

MIS-A

Multisystem inflammatory syndrome in adults

RT-PCR

Real-time polymerase chain reaction

SAD

Specific antibody deficiency

SARS-CoV-2

Severe Acute Respiratory Syndrome Coronavirus 2

SCID

Severe combined immune deficiency

WAS

Wiskott-Aldrich syndrome

XLA

X-linked Agammaglobulinemia

Authors’ contributions

Substantial contribution to the acquisition of the data were provided by L.M. Castano-Jaramillo, M.A. Yamazaki-Nakashimada, P.M. O’Farrill-Romanillos, D. Muzquiz Zermeño, S.C. Scheffler Mendoza, E. Venegas Montoya, J.A. García Campos, L.M. Sánchez-Sánchez, L.B. Gámez González, J.M. Ramírez López, J.C Bustamante Ogando, E. Vásquez-Echeverri, E.A. Medina Torres, G. Lopez-Herrera, L. Blancas Galicia, L. Berrón Ruiz, A.T Staines-Boone, S.E. Espinosa-Padilla, N.H. Segura Mendez, and S.O. Lugo Reyes. Substantial contribution to the conception of the work, analysis of the data, and drafting the manuscript were provided by L.M. Castano-Jaramillo and S.O. Lugo Reyes. Yamazaki-Nakashimada, P.M. O’Farrill-Romanillos, D. Muzquiz Zermeño, S.C. Scheffler Mendoza, E. Venegas Montoya, J.A. García Campos, L.M. Sánchez-Sánchez, L.B. Gámez González, J.M. Ramírez López, J.C Bustamante Ogando, E. Vásquez-Echeverri, E.A. Medina Torres, G. Lopez-Herrera, L. Blancas Galicia, L. Berrón Ruiz, A.T Staines-Boone, S.E. Espinosa-Padilla, and N.H. Segura Mendez critically revised the manuscript. All authors read and approved the final manuscript.

Funding

No funding was received for this manuscript.

Data availability

All data generated or analyzed during this study are included in this published article.

Declarations

Ethics approval

The study was approved, as part of a larger project, by the Research and Ethics committees at the National Institute of Pediatrics.

Consent to participate and to publish.

Written informed consent was obtained from all patients or their legal guardians.

Competing interests

We have no competing interests to disclose.