Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | Slc1a6-EGFP | Gong et al., 2003 | MMRRC: 012845-UCD | (Tg(Slc1a6-EGFP) HD185Gsat/Mmucd) |
| Strain, strain background (Mus musculus) | Pcp2-creERT2 | Institut Clinique de la Souris, France Jackson Laboratory | MGI:97508; ICS: 0273 | (Tg(Pcp2-creERT2)17.8.ICS) |
| Strain, strain background (Mus musculus) | Ai14 | The Jackson Laboratory | JAX: 007908 | (Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J) |
| Strain, strain background (Mus musculus) | C57BL/6 | The Jackson Laboratory Janvier Labs Charles River |
JAX: 00055 Janvier: C57BL/6JRj CR: C57BL/6NCrl |
|
| Antibody | Goat anti-ZebrinII/Aldolase C (Goat polyclonal) | Santa Cruz Biotechnology | Cat# sc-12065 RRID:AB_2242641 RRID:AB_2315622 |
1:1000 |
| Antibody | Mouse anti- Calbindin D-28K (Mouse monoclonal) | Swant | Cat# 300 RRID:AB_10000347 |
1:10,000 |
| Antibody | Guinea pig anti-VGluT2 (Guinea pig polyclonal) | Millipore | Cat# AB2251-l RRID:AB_2665454 |
1:2000 |
| Antibody | Rabbit anti-RFP (Rabbit polyclonal) | Rockland | Cat# 600-401-379 RRID:AB_2209751 |
1:1000 |
| Antibody | Cy3 Streptavidin | Jackson ImmunoResearch | Cat# 016-160-084 RRID:AB_233724 |
1:1000 |
| Antibody | Cy3-AffiniPure Donkey anti-Mouse (Mouse polyclonal) | Jackson ImmunoResearch | Cat# 715-165-150 RRID:AB_2340813 |
1:1000 |
| Antibody | Alexa Fluor 488-AffiniPure Donkey anti-Guinea Pig (Guinea pig polyclonal) | Jackson ImmunoResearch | Cat# 706-545-148 RRID:AB_2340472 |
1:1000 |
| Antibody | Donkey anti-goat Daylight 488 (Goat polyclonal) | Jackson ImmunoResearch | Cat# 705-486-147 RRID:AB_2616594 |
1:500 |
| Antibody | Cy3-AffiniPure Donkey Anti-Rabbit (Rabbit polyclonal) | Jackson ImmunoResearch | Cat# 711-165-152 RRID:AB_2307443 |
1:500 |
| Chemical compound, drug | 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI) | Thermo Fisher Scientific | Cat# D3571 RRID:AB_2307455 |
|
| Chemical compound, drug | Paraformaldehyde (PFA) | Millipore | Cat# 104005 | 4% |
| Chemical compound, drug | Biocytin | Sigma-Aldrich | Cat# B4261 | 1% |
| Chemical compound, drug | Evans Blue | Sigma-Aldrich | Cat# E2129 | 0.5% |
| Chemical compound, drug | Picrotoxin | Hello Bio Ltd | Cat# HB0506 | 100 µM |
| Chemical compound, drug | NBQX | Hello Bio Ltd | Cat# HB0442 | 10 µM |
| Chemical compound, drug | D-AP5 | Hello Bio Ltd | Cat# HB0225 | 50 µM |
| Chemical compound, drug | Mineral oil | Sigma-Aldrich | Cat# M3516 | |
| Chemical compound, drug | Tamoxifen | Sigma-Aldrich | Cat# T5648 | |
| Software, algorithm | FIJI (ImageJ) | National Institute of Health | RRID:SCR_002285 | |
| Software, algorithm | MATLAB 2008, 2016 | MathWorks | RRID:SCR_001622 | |
| Software, algorithm | ZEN digital Imaging for Light Microscopy | ZEISS | RRID:SCR_013672 | |
| Software, algorithm | Leica Application Suite X (LAS X) | Leica Microsystems | RRID:SCR_013673 | |
| Software, algorithm | Clampfit 10 | Molecular Devices | RRID:SCR_011323 | |
| Software, algorithm | Patchmaster | HEKA Electronics | RRID:SCR_000034 | |
| Software, algorithm | GraphPad Prism | GraphPad Software | RRID:SCR_002798 | |
| Software, algorithm | Compensatory eye movements analysis | Schonewille group | https://github.com/MSchonewille/iMove | |
| Software, algorithm | Eyeblink conditioning analysis | Neurasmus B.V. Rotterdam | RRID:SCR_021043 | |
| Other | Leica SM2000 R sliding microtome | Leica Biosystems | RRID:SCR_018456 | |
| Other | P-1000 Puller | Sutter Instrument | RRID:SCR_021042 | |
| Other | LSM 700 laser scanning confocal | ZEISS | RRID:SCR_017377 | |
| Other | SP5 confocal | Leica Microsystems | RRID:SCR_020233 | |
| Other | SP8 confocal | Leica Microsystems | RRID:SCR_018169 | |
| Other | Axio Imager.M2 | ZEISS | RRID:SCR_018876 |