Fig. 4.

Expression of XLOC_030252 in human and mouse hematopoietic cells. a,b Expression of XLOC_030252 in different stages of human hematopoietic cells. RNA-seq gene tracks showing RNA expression in human hematopoietic cells at XLOC_030252 locus a. Each cell type was defined by Casero et al. [28] as follows: HSC, CD34⁺CD38^neglin^neg; lymphoid-primed multipotent progenitors (LMPP), CD34⁺CD38⁺CD10^negCD45RA⁺CD62L^highlin^neg; common lymphoid progenitor (CLP), CD34⁺CD38⁺CD10⁺CD45RA⁺lin^neg; Thy1, CD34⁺CD7^negCD1a^negCD4^negCD8^neg; Thy2, CD34⁺CD7⁺CD1a^negCD4^negCD8^neg; Thy3, CD34⁺CD7⁺CD1a⁺CD4^negCD8^neg; Thy4, CD4⁺CD8⁺; Thy5, CD3⁺CD4⁺CD8^neg; and Thy6, CD3⁺CD4^negCD8⁺. Two scales were included, because expression levels of XLOC_030252 and protein-coding genes were different. Expression level of XLOC_030252 in duplicate samples reported by Casero et al. [28] were shown by the TPM values. b,c RNA-seq dataset reported by Sun et al. [29] was used to analyze RNA expression in mouse HSCs collected at different ages (4 and 24 months) at the genomic locus conserved with human XLOC_030252 sequence. d The RNA expression of putative paralog of XLOC_030252 in different stages of mouse hematopoietic cells were measured by qRT-PCR and normalized to β2M expression. Long-term HSC (LT-SHC), short-term HSC (ST-HSC), multi potent progenitor (MPP), common lymphoid progenitor (CLP), DN1–4, DP, CD4 single-positive and CD8 single-positive cells. Fold-change values compared with LT-HSC are shown as the mean ±standard deviation (SD) of duplicate samples. See Supplementary Materials for the definition of each population. e Mouse lymphoid progenitor cell line EML was transduced with control shRNA (shLuc) or shRNA targeting mouse Tal1 (sh1 and sh2) by lentivirus infection. RNA expression of putative paralog of human XLOC_030252 was measured by qRT-PCR and normalized to β2M expression. Fold-change values compared with the control are shown as the mean ± SD of duplicate samples. **p < 0.01 by two-sample, two-tailed t-test.