Fig. 6.

δ-TT-induced ROS are involved in paraptotic vacuolization and MAPK activation. a A375 and BLM cells were incubated with 5 mM NAC for 2 h and then treated with 15 μg/mL δ-TT for 12 h. Cell morphology was analyzed by light microscopy. Scale bars are 20 µm. b A375 and BLM cells were incubated with 5 mM NAC for 2 h and then treated with 15 μg/mL δ-TT for 24 h. The expression levels of pJNK, pP38 and pERK1/2 were evaluated via western blot analysis. Tubulin was used as a housekeeping protein control. Three experiments have been performed