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. 2021 Mar 29;24(2):387–398. doi: 10.1007/s10456-021-09777-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — SMAD6 is required for homeostatic endothelial cell flow-mediated alignment and polarization. a Representative panels of HUVEC stained with VE-cadherin (green, junctions) and DRAQ7 (white, nucleus) under indicated conditions with indicated treatments. White arrow, flow vector. Scale bar, 20 μM. b Quantification of cell axis ratio. Statistical analysis, One-way ANOVA; ****p ≤ 0.0001. N = 3, representative experiment shown. c Representative panels of HUVEC stained with GM130 (red, Golgi) and DAPI (white, nucleus) under indicated conditions with indicated treatments. White arrow, flow vector. Scale bar, 50 μM. d Quantification of Golgi localization relative to nucleus in indicated conditions. ≥ 30 cells/condition were analyzed. Statistical analysis, One-way ANOVA; **p ≤ 0.01; ***p ≤ 0.001. NT non-targeting (control) siRNA