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. 2013 Nov 6;28(6):345–351. doi: 10.1007/s12250-013-3350-5

PNKP knockdown by RNA interference inhibits herpes simplex virus-1 replication in astrocytes

Lei Yue 1, Sujie Guo 1, Xia Cao 2, Ying Zhang 1, Le Sun 1, Longding Liu 1, Min Yan 1, Qihan Li 1,✉
PMCID: PMC8208462  PMID: 24213989

Abstract

Herpes simplex virus-1 (HSV-1) is a major pathogen that causes various central nervous system (CNS) diseases, including herpes simplex encephalitis and meningitis. According to recent studies, PNKP significantly affects the proliferation of HSV-1 in astrocytes. Here, we used viral proliferation curves to confirm the significant inhibitory effects of PNKP on HSV-1 proliferation. PNKP downregulation was also confirmed by analyzing the transcription of viral genes. We found that PNKP downregulation affects the viral DNA copy number. This study preliminarily confirms that PNKP affects viral proliferation by affecting HSV-1 genome cyclization. These results also suggest that astrocytes play a specific role in preventing HSV-1 infection.

Keywords: Herpes simplex virus I (HSV-1), Replication, PNKP, Cyclization, Primary culture, Monkey

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