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. 2021 May 25;11(15):7379–7390. doi: 10.7150/thno.58781

Figure 4.

Figure 4

Cytokine expression level changes with knockdown of TRPV2 expression in PBAMs following SARS-CoV-2 S treatment in the presence of febrile temperature. (A, B) Depletion of TRPV2 in PBAM using specific shRNAs was performed via lenti-virus transfection. qRT-PCR (A) and western blot assays (B) confirmed TRPV2 knockdown in macrophages. Data are shown as the mean ± SD (n = 3). **p < 0.01. (C-E) Cytokine array analysis showing significant changes in the expression of multiple proteins. Twelve cytokines, including IFN-α (p < 0.05), IFN-γ (p < 0.05), IL-13 (p < 0.01), IL-α (p < 0.05), TNF-α (p < 0.01), IL-10 (p < 0.01), IL-18 (p < 0.01), IL-2 (p < 0.01), IL-4 (p < 0.05), IL-17A (p < 0.05), MCP-1 (p < 0.05), and IL-15 (p < 0.05) exhibited robust decreases in His - S-RBD-ShTRPV2 macrophages vs His - S-RBD-NC macrophages at 39.5 °C. Data are shown as the mean ± SD (n = 3). *p < 0.05. **p < 0.01. NS: not significant.