Figure 8. Plxnb2 mutant CGNs in culture display aberrant tangential migration.
(A) 15-minute-time-lapse confocal still images at t 0, 1, 2, 3, 4, and 5 hr showing GFP+ CGNs migrating from a DIV1 explant (located on the left side of the images). Scale bars 50 μm. (B) Bipolar CGNs migrate at an equal speed. (ctl: 26.75 ± 1.23 μm/h vs. mut: 27.77 ± 1.25 μm/h, MWU(973) p=0.75, not significant). Forty-five bipolar cells were tracked for each condition, from 13 ctl and 11 mut cultures from five independent experiments. Error bars represent SEM. (C) En1Cre;Plxnb2fl/fl CGNs cover more distance (ctl 9.42 ± 1.60% vs. mut 32.21 ± 3.10%, MWU(306) p<0.0001) and spend more time (ctl 15.15 ± 1.77% vs. mut 35.86 ± 2.80%, MWU(352.5) p<0.0001) going in negative direction (toward instead of away from the explant). Forty-five bipolar cells were tracked for each condition, from 13 ctl and 11 mut cultures. Error bars represent SEM. (Figure 8—source data 1).