Fig. 4.

CD8-depletion and rechallenge.

A) Absolute counts of CD8⁺ cells in the blood. CD8 T cells were depleted prior to rechallenge in two of four macaques that were initially infected during the 3rd trimester of pregnancy. (B) Serum viral loads following secondary challenge. The four macaques were rechallenged with the heterologous ZIKV strain PRVABC-59. (C-D) Plaque reduction neutralization test (PRNT) was used to assess anti-ZIKV neutralizing humoral immunity before and after rechallenge. Percent neutralization curves were calculated for all 4 animals at both time points (C). From the curves represented in C, we calculated the neutralization titer which resulted in an 80% reduction in plaques (NT80) (D).