Figure 5. Repeated antigen stimulation alters localization of LN Trm cells.

(A–D) Mice containing mixed congenically distinct populations of 1M (CD90.1/.1, seeded with 10⁴ naive P14) and 4M (CD90.1/.2 seeded with 10⁵ 3M P14) P14s were injected with bolus IV administration of CD90.1-PE (red) and CD90.2-APC (white). Approximately 5 hr post-injection, organs were isolated and two-photon microscopy was performed on whole mLN (A–B) or iLN (C–D) explants ex vivo. The LN surrounding collagen capsule (pseudocolored blue) was captured with secondary harmonic generation (SHG). Representative of two independent experiments, n = 3–4 mice/group.