Fig. 5.

C-terminal domain of OsJAZ11 regulates root growth. Plant phenotype of 30-day-old OsJAZ11-GUS (a, d), OsJAZ11ΔC-GUS (b, e) translational reporter lines and WT under +P (a, b) and −P (d, e) conditions. For imaging three representative plants of each line were stacked together. Scale bar = 10 cm. c Quantitation of root lengths of WT, OsJAZ11-GUS and OsJAZ11ΔC-GUS lines under +P conditions. f Quantitation of root lengths of WT, OsJAZ11-GUS and OsJAZ11ΔC-GUS lines under −P conditions. Each bar represents mean of ten biological replicates with standard error. Significant differences between WT and transgenics were evaluated by Student’s t test. Asterisks; *, ** and *** indicate P values, ≤ 0.05, 0.01 and 0.001, respectively (n = 10). g-h Fluorometric quantitation of GUS activity in 30-day-old OsJAZ11-GUS (L3, L12, L14) and OsJAZ11ΔC-GUS (L1, L2) transgenics under +P and –P conditions. Each bar represents mean of six biological replicates with standard error. Different letters on top of each bar denotes significant differences determined by one-way ANOVA followed by Duncan’s multiple comparison test (α < 0.05) (n = 6)