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. 2021 May 19;24(6):102554. doi: 10.1016/j.isci.2021.102554

Figure 5.

Figure 5

Neutralization of GDF15 does not modulate weight loss, anorexia, or mortality induced with a sublethal dose of LPS

(A) Plasma GDF15 levels in mice after 5 mg/kg LPS sublethal dose (n = 5 per time point).

(B) Study schematic.

(C) Body weight after LPS and IgG control or mAB2 injection. Body weight is represented as % of the day 0 body weight.

(D) Cumulative food intake after after LPS and IgG control or mAB2 injection.

(E) Health score representing overall condition of animals by their appearance, natural behavior, provoked behavior, and body condition scores after LPS and IgG control or mAB2 injection.

(F) Kaplan-Meier survival curve indicating % survival after LPS injection with IgG control or mAB2 treatments. log rank test p = 0.6. ∗ p value < 0.05, ∗∗∗ < 0.001 vs. LPS + IgG; ^ p value < 0.05, ^^ p value < 0.01, ^^^ p value < 0.001 vs. LPS + mAB2. Data in C and D were analyzed using a longitudinal mixed-effects ANOVA. Failure time data were compared between treatment groups with Kaplan-Meier analysis using the log rank test. Data represented as mean ± SEM. N = 13 per group.

AU, arbitrary unit; BW, body weight; FI, food intake; GDF15, growth differentiation factor 15; HS, health score; IgG, immunoglobulin G; LPS, lipopolysaccharide; PBS, phosphate-buffered saline; SEM, standard error of the mean.