Figure 1.

Serum sFRP1 is reduced in SSc. (a) Serum levels of sFRP1 were measured by ELISA (RayBiotech) in diffuse SSc patients or healthy controls (n = 10). *Significantly different from HC; Mann-Whitney U test. 1B). Healthy dermal fibroblasts were cultured in vitro and transfected with 75 nM of microRNA27a-3p or matched scramble for 48 h after which the supernatant was collected and sFRP-1 measured by specific ELISA (*significant difference P = 0.028 Mann-Whitney U test n = 4). 1 C). SSc and healthy control fibroblasts were measured for microRNA27a-3p by Taqman PCR and normalized to RNU44 and data shown as fold change over healthy control (*significant difference P = 0.011; Mann-Whitney U test; n = 5) Data is the mean and SD