Fig. 4.
Effect of lin-32 on the expression of candidate terminal selectors.(A,B) Loss of candidate terminal selector expression in lin-32(tm1446)-null mutants. Reporters were: lin-11[ot958(lin-11::gfp::FLAG)], ceh-32[ot1040(ceh-32::gfp)], unc-86[ot879(unc-86::nNeonGreen)], otIs339 (ceh-43fosmid::gfp) and unc-42 [ot958(unc-42::gfp)]. (A) Representative images of wild-type and mutant phenotypes; red dashed outlines indicate location of the defective cell; blue dashed outlines indicate outline of pharynx; red arrows indicate nerve ring. (B) Percentage of animals that displayed indicated marker gene defect in the left (L) or right (R) neuron of a bilateral neuron pair. NeuroPAL (otIs696) was used in the background of all of the strains mentioned herein in order to identify neurons expressing the terminal selector. Circles indicate bilateral homologs of the respective neuron class (L>0: expression only in left neuron; 0<R: expression only in right neuron; L=R: expression in both neurons, i.e. wild type). Scale bars: 10 µm.