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. 2012 Jun 9;27(3):194–203. doi: 10.1007/s12250-012-3248-7

Molecular characterization of viral G gene in emerging and Re-emerging areas of rabies in China, 2007 to 2011

Shu-Lin Lang 1,2, Xiao-Yan Tao 2, Zhen-Yang Guo 2,3, Qing Tang 2,✉, Hao Li 2, Cui-Ping Yin 2, Ying Li 1, Guo-Dong Liang 2
PMCID: PMC8218082  PMID: 22684474

Abstract

In recent years (2007 to 2011), although the overall number of rabies cases in China has decreased, there is evidence of emerging or re-emerging cases in regions without previous rabies cases or with low incidence of rabies. To investigate the origin and the factors affecting the spread of rabies in China, specimens were collected from 2007 to 2011 from provinces with emerging and re-emerging cases and tested for the presence of the rabies virus. Positive specimens were combined with sequences from GenBank to perform comparisons of homology and functional sites, and to carry out phylogenetic analyses. Out of these regions, five provinces had 9 positive specimens from canine and cattle, and 34 canine or human specimens were obtained from previously high-incidence provinces. Complete sequences of G gene were obtained for these samples. Homology of the sequences of these 43 specimens was 87%–100% at the nucleotide level and 93.7%–100% at the amino acid level. These G gene sequences were combined with reference sequence from GenBank and used to construct a phylogenetic tree. The results showed that 43 specimens were all assigned to China clade I and clade II, with all specimens from emerging and re-emerging areas placed within clade I. Specimens isolated from Shanxi and Inner Mongolia in 2011 were distinct from previously-isolated local strains and had closer homology to strains from Hebei, Beijing and Tianjin whereas new isolates from Shanghai were tightly clustered with strains isolated in the 1990s. Finally, Shaanxi isolates were clustered with strains from adjacent Sichuan. Our results suggest that the rabies cases in emerging and re-emerging areas in China in the last 5 years are a consequence of the epidemic spreading from of neighboring provinces and regions experiencing a serious epidemic of rabies.

Key words: Rabies virus, G gene, Genetic variation, Molecular characteristics

Footnotes

Foundation items: National Department Public Benefit Research Foundation (201103032); Pathogens Network Monitoring Technology Research (2008ZX10004-008).

References

  • 1.Badrane H., Tordo N. Host switching in Lyssavirus history from the Chiroptera to the Carniv or a orders. J Virol. 2001;75(17):8096–8104. doi: 10.1128/JVI.75.17.8096-8104.2001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 2.Gong W., Jiang Y., Za Y., et al. Temporal and spatial dynamics of rabies viruses in China and Southeast Asia. Virus Res. 2010;150(1–2):111–118. doi: 10.1016/j.virusres.2010.02.019. [DOI] [PubMed] [Google Scholar]
  • 3.Hall T. A. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nuc Acids Symp Ser. 1999;41:95–98. [Google Scholar]
  • 4.Hillis D. M., Bull J. J. An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systemat Biol. 1993;42:182–192. [Google Scholar]
  • 5.Hu R. L., Tang Q., Tang J. R., et al. Rabies in China: An Update. Vector-borne zoonot. 2009;9(1):1–4. doi: 10.1089/vbz.2008.0046. [DOI] [PubMed] [Google Scholar]
  • 6.Jackson A. C. Pathogenesis. In: Jackson A. C., Wunner W. H., editors. Rabies. London: Elsevier Science; 2002. pp. 246–282. [Google Scholar]
  • 7.Lei Y. L., Wang X. G., Li H., et al. New animal hosts of rabies virus in mountain areas in Zhejiang province. Chin J Epedemiol. 2009;30(4):344–347. [PubMed] [Google Scholar]
  • 8.Li H., Shen X. X., Tang Q. Analysis On epidemic characteristics of rabies in China, in 2007. Chin J Epedemiol. 2009;30(8):874–875. [Google Scholar]
  • 9.Li H., Tao X. Y., Song M., et al. Survey and analysis of infection rate of dog rabies in the regions with high incidence of human rabies. Chinese J Exp Chin Virol. 2008;22(3):161–164. [PubMed] [Google Scholar]
  • 10.Mellquist J. T., Kasturi L., Spitalnik S. L., et al. The amino acid following an Asn-X-Ser/Thr sequon is animportant determinant of N-Linked core glycosylation efficiency. Biochemistry. 1998;37:6833–6837. doi: 10.1021/bi972217k. [DOI] [PubMed] [Google Scholar]
  • 11.Meng S., Xu G., Wu X., et al. Transmission dynamics of rabies in China over the last 40 years: 1969–2009. J Clin Virol. 2010;49(1):47–52. doi: 10.1016/j.jcv.2010.06.014. [DOI] [PubMed] [Google Scholar]
  • 12.Nel L. H., Markotter W. Lyssaviruses. Crit Rev Microbiol. 2007;33(4):301–324. doi: 10.1080/10408410701647602. [DOI] [PubMed] [Google Scholar]
  • 13.Patricia L. D., Edward C. H., Florence L., et al. Phylogeography, Population Dynamics, and Molecular Evolution of European Bat Lyssaviruses. J Virol. 2005;79:10487–10497. doi: 10.1128/JVI.79.16.10487-10497.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 14.Rustici M., Bracci L., Loci L., et al. A model of the rabies virus glycoprotein active site. Biopolymers. 1993;33:961–969. doi: 10.1002/bip.360330612. [DOI] [PubMed] [Google Scholar]
  • 15.Song M., Tang Q., Wang D. M., et al. Epidemiological investigations of human rabies in China. BMC Infect Dis. 2009;9:210. doi: 10.1186/1471-2334-9-210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Song M., Tang Q., Xu Z., et al. Analysis on the factors related to rabies, epidemic to China, in 2005. Chin J Epedemiol. 2006;27(11):956–959. [PubMed] [Google Scholar]
  • 17.Tamura K., Peterson D., Peterson N., et al. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol Biol Evol. 2011;28:2731–2739. doi: 10.1093/molbev/msr121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18.Tang Q., Lillian A. O., Charles E., et al. Sequencing and Position Analysis of the Glycoprotein Gene of Four Chinese Rabies Viruses. Virol Sin. 2000;15(1):22–33. [Google Scholar]
  • 19.Thompson J. D., Gibson T. J., Plewniak F., et al. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997;25(24):4876–4882. doi: 10.1093/nar/25.24.4876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 20.WHO expert consultation on rabies: First Report. 2005. WHO technical report series #931. Geneva: World Health Organization, 1–88. [PubMed]
  • 21.Wunner W. H., Reagan K. J., Koprowski H. Characterization of saturable binding sites for rabies virus. J Virol. 1984;50:691–697. doi: 10.1128/jvi.50.3.691-697.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 22.Yao W. R., Pan G. Q., Xiong C. L., et al. Detection and genetic characterization of rabies virus from human patients. Virol Sin. 2007;22(4):307–315. doi: 10.1007/s12250-007-0007-2. [DOI] [Google Scholar]
  • 23.Zhang K. S., Guo J. H., Xiang M., et al. Diagnosis and molecular characterization of rabies virus from a buffalo in China: a case report. Virol J. 2011;8:101. doi: 10.1186/1743-422X-8-101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 24.Zhang Y. Z., Xiong C. L., Xiao D. L. Human rabies in China. Emerg Infect Dis. 2005;11:1983–1984. doi: 10.3201/eid1112.040775. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25.Zhao J., Liu Y., Zhang S., et al. Analysis of an outbreak of human rabies in 2009 in Hanzhong District, Shaanxi province, China. Vector-Borne Zoonot. 2011;11(1):59–68. doi: 10.1089/vbz.2009.0190. [DOI] [PubMed] [Google Scholar]
  • 26.Zhu J. H., Wang J. L., Cai B., et al. Immunogenicity and relative attenuation of different-rabies recombinants. Arch Virol. 1996;141:1055–1065. doi: 10.1007/BF01718609. [DOI] [PubMed] [Google Scholar]

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