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. 2012 Jan 22;27(1):48–56. doi: 10.1007/s12250-012-3227-z

Development of multiple elisas for the detection of antibodies against classical swine fever virus in pig sera

Zhen-hua Yang 1, Ling Li 1, Zi-shu Pan 1,✉
PMCID: PMC8218085  PMID: 22270806

Abstract

The major immunogenic proteins (Erns, E2 and NS3) of classical swine fever virus (CSFV) (Shimen strain) were expressed in E. coli and purified by affinity chromatography. The recombinant antigens were applied to develop multiple enzyme-linked immunosorbent assays (ELISAs) for the detection of specific antibodies in pig sera. Optimum cut-off values were determined by receiver operating characteristic (ROC) analysis after testing 201 sera of vaccinated pigs and 64 negative sera of unvaccinated piglets. The multiple ELISAs were validated with 265 pig sera yielding high sensitivity and specificity in comparison with the virus neutralization results. The results demonstrated that multiple ELISAs can be a valuable tool for the detection of CSFV infection and serological surveys in CSFV-free countries or for the evaluation of the antibody responses in pigs induced by a live attenuated C-strain vaccination.

Key words: Classical swine fever virus, Recombinant antigens, ELISA

Footnotes

Foundation items: the National Natural Science Foundation of China (30771597); the Key Project of Science and Technology of Wuhan (200720422141).

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Articles from Virologica Sinica are provided here courtesy of Wuhan Institute of Virology, Chinese Academy of Sciences

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