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. Author manuscript; available in PMC: 2021 Jul 20.
Published in final edited form as: Nature. 2021 Jan 20;590(7847):655–659. doi: 10.1038/s41586-020-03172-w

Extended Data Figure 7. Initiation of BIR immobilizes rPolII on H-On transcribed genes.

Extended Data Figure 7.

a, Location of primer pairs homologous to GAL1 promoter (pair 1), TSS (transcription start site) (pair 2) and the middle of HIS3 gene (pair 3) used for qPCR (see Supplementary Table 4 for primer sequences). b, RNA PolII enrichment at PGAL1-HIS3 in H-On and Co-D orientations measured at indicated times following galactose addition. The means of rPolII enrichment ± SD indicated by error-bars (n=3 independent biological experiments). c, Transcription levels of PGAL1-HIS3 8 hr post-DSB detected by HIS3-specific primers and normalized to ACT1 mRNA control. The means ± SD (n=3 independent biological repeats), indicated by error-bars. (**= statistically significant difference (p=0.0033) determined by t test, two tailed).