Table 2.

Kinetic constants for 4-hydroxynonenal of human AKRs and ALDHs.

Enzyme	Km (μM)	kcat (min−1)	kcat/Km (min−1 μM−1)	Assay Condition (pH and °C)	References
AKR1B10	30.9	121	3.9	pH 7.0, 35 °C	[45]
	31	119	3.8	pH 7.0, 35 °C	[47]
	4.7	27	6.1	pH 7.4, 25 °C	[48]
AKR1B1	22	102	4.6	pH 7.0, 25 °C	[49]
	31	16.6	1.2	pH 7.0, 25 °C	[50]
	716	50	0.07	pH 6.4, 0.3 M Li2SO4, 35 °C	[47]
AKR1B15	2.2	5.2	2.5	pH 7.0, 25 °C	[5]
AKR1C1	34	8.8	0.27	pH 7.0, 37 °C	[51]
ALDH1A1	27	10	0.38	pH 8.0, 25 °C	[40]
ALDH1A2	7.5	31	4.1	pH 8.0, 25 °C	[40]
ALDH1A3	40	7.4	0.185	pH 8.0, 25 °C	[40]