Antibiotic treatment increases yellowness of carotenoid feather coloration in male greenfinches (Chloris chloris)

Abstract

Carotenoid plumage coloration is an important sexually selected trait in many bird species. However, the mechanisms ensuring the honesty of signals based on carotenoid pigments remain unclear. It has recently been suggested that intestinal integrity, which is affected by gut parasites and microbiota and influences nutrient absorption and acquisition, mediates the relationship between carotenoid ornamentation and individual quality. Here, we test whether carotenoid plumage coloration in greenfinches (Chloris chloris) is affected by the treatment of an antibiotic or an antiparasitic drug. We captured wild greenfinches (N = 71) and administered anticoccidial medication toltrazuril (TOLTRA) to one group, antibiotic metronidazole (METRO) to the second group to target trichomonosis, and the third group received no medication. In the METRO group, feathers grown during the experiment had significantly higher chroma of yellow parts, but there was no effect of TOLTRA on feather chroma. The results suggest that METRO increased the efficiency of carotenoid modification or deposition to the feathers rather than nutrient acquisition and/or freed energy resources that could be invested in coloration. Alternatively, though not measured, METRO might have affected microbial community and host physiology as microbial metabolites can modulate mitochondrial and immune function.

Introduction

Carotenoid ornaments are important sexual signals that play a crucial role in mate choice in many vertebrate species, including birds. Females often prefer males with brighter and more intense carotenoid feather coloration, and accordingly, these males are more likely to attain a mate and invest more in taking care of nestlings¹. In some species, more colourful males live longer and have higher lifetime reproductive success². The honesty of sexually selected signals must be ensured in order for them to contain valuable information and avoid cheating³. However, the mechanisms that guarantee the honesty of carotenoid ornaments are still not clear.

Over decades of research on carotenoid feather coloration, several non-mutually exclusive mechanisms have been suggested. For example, feather coloration has been associated with oxidative stress and antioxidant capacity^4–6. However, multiple studies have failed to replicate these results^7–9, and meta-analyses have found only weak relationships between these variables and carotenoid coloration¹⁰. It has also been proposed that the conversion of carotenoids is sensitive to the functionality of mitochondria, as the enzyme responsible has been suggested to reside within the mitochondrial membrane¹¹. This is one example of the “shared pathway hypothesis”, according to which signal honesty could also be maintained when production of the signal is tied to the functionality of vital cellular proceses¹². In other studies, carotenoids have been associated with resistance to parasites and parasite load^13,14. A recent meta-analysis found that metabolically converted carotenoids were related to parasite resistance and reproductive and parental quality, but unconverted carotenoids were not¹⁵.

Parasites can affect carotenoid coloration through the compromised absorption of carotenoids in the gut. Many gut parasites cause inflammation of the gut surface that can reduce the absorption of carotenoids and overall energy acquired from the food¹⁶. Among birds, protozoan coccidian parasites are widely distributed gut parasites that have been shown to affect digestive efficiency. Coccidia are intracellular parasites, and their asexual and sexual multiplication inside of the intestine's epithelial cells damages the animal’s ability to acquire nutrients from food^17,18. In poultry, coccidian parasites cause extensive damage to the intestinal villi¹⁹, inflammation of the gut surface²⁰, leaky gut¹⁷, and reduced digestive efficiency^18,21. The damage caused by coccidiosis to the intestines has been associated with malabsorption of carotenoids^16,22,23 and other nutrients^17,18. In captive greenfinches (Chloris chloris), goldfinches (Carduelis carduelis), and Nashville warblers (Vermivora ruficapilla), Isosporan coccidia caused thickening of the intestinal wall^24,25, which in turn correlated negatively with weight²⁵. Coccidia have been shown to affect carotenoid coloration in wild birds^14,26. However, it is unclear if these effects occur through general condition decline or specifically impact gut integrity.

Other parasites, such as Trichomonas gallinae and pathogenic bacteria, may not directly affect intestinal integrity, but decrease the amount of energy available to the individual from food. Protozoan T. gallinae is a parasite that causes lesions in the upper digestive tract in several species of birds, which in severe cases can block the esophagus and make the passage of food impossible²⁷. Avian trichomonosis caused by T. gallinae is historically common in pigeons and raptors. However, since 2005, it has been reported as an emerging disease in greenfinches in Europe, causing epidemics that have resulted in high mortality and a decline in populations of wild greenfinches^28,29. Avian trichomonosis is an example of a relatively recent phenomenon in which a parasite spills over to another taxonomic group with drastic effects on a new host species.

Due to their well-established effect on growth rate and feed conversion, antibiotics are often administered prophylactically in poultry^30,31. It is not entirely clear how antibiotic growth promoters elicit its beneficial effects, but it has been shown that they do not exert growth-promoting properties in germ-free animals. Thus, the antimicrobial effect is most likely the key factor in enhancing growth performance. Suggested mechanisms of antibiotic growth promoters include reducing the severity of subclinical infections, reducing the microbial use of nutrients, and improving absorption of nutrients. Antibiotic growth promoters in broiler feed has reported to cause shifts in the gut microbiota composition. These shifts can affect the morphology of the gut wall, modulate immune reactions, and impact the host’s energy expenditure (reviewed in³⁰). For example, antibiotic supplementation (methylene disalicylate and virginiamycin) with feed increased the body weight of broiler chickens compared to controls at several time points until seven weeks of age and resulted in morphological changes to the intestines of birds³². In chickens coinfected with Eimeria and Clostridium, antibiotic supplementation (methylene disalicylate and virginiamycin) resulted in deeper intestinal crypts, decreased intestinal lesion, and greater weight gain than in unsupplemented control 22 days post-hatch³³. Following the European Commission’s ban on the use of antibiotics as growth promoters (January 1st, 2006), an increase of some health conditions in poultry has been described, such as necrotic enteritis and dysbiosis³⁰.

Although several studies in poultry have detected the effect of coccidian parasites on decreased nutrient absorption and carotenoid levels, few studies have considered this mechanism in the coloration of wild birds. In contrast to poultry, where the most economically relevant coccidian parasites are from the genus Eimeria, the predominant coccidian genus in many wild species is Isospora, which might have different effects on the host. However, some studies in wild birds have revealed similar results to poultry. Experimental infection of greenfinches with coccidian parasites showed parallel patterns of decreased carotenoid, serum albumin, and triglycerides in serum and decreased carotenoids in the feathers of greenfinches, which points to reduced digestive and absorption capacity¹⁴. Infection with Isospora sp. coccidians increased fat content in the faeces of greenfinches³⁴. Carotenoids are fat-soluble, thus carotenoid deficiency and malabsorption of lipids are likely related³⁵. In house finch (Haemorhous mexicanus) redness of carotenoid coloration correlated with digestive efficiency —individuals with redder ornaments absorbed more fats from their diet³⁶.

The effect of other infections, such as trichomonosis, on carotenoids acquired through diet, has not received much attention. However, in greenfinches, it has been shown that melanin, but not carotenoid coloration, predicted mortality, and black parts of tail feathers became darker in generations emerged subsequent to the outbreak of trichomonosis epidemic, while no change was observed in the yellow parts of feathers³⁷. Therefore, different parasites might induce selection pressure on different components of bird coloration. Apart from the growth rate in poultry, not much is known about how the microbiome affects the production of condition-dependent traits. Antibiotics have a well-known effect on altering the composition of the gut microbiome. However, to our knowledge, the impact of experimental administration of antibiotics on sexually selected traits has not been tested previously in wild birds.

This study aimed to test whether treatment with two different antimicrobial medications affected plasma carotenoid levels and carotenoid-based feather coloration in greenfinches. The chroma of the yellow parts of feathers is related to carotenoid content in the feathers of greenfinches. Greenfinches in our study population are naturally infected by coccidian parasites (Isospora spp.)³⁹, and we previously detected characteristic symptoms of trichomonosis in some birds that have died in the aviary⁴⁰. We treated the birds with the anticoccidial drug Toltrazuril (TOLTRA), which is designed specifically to treat coccidiosis and lacks known effects on microbes other than apicomplexans⁴¹. Toltrazuril affects the respiratory mechanism of coccidia by interfering with nuclear division and mitochondrial activity⁴². The other group received nitroimidazole group antibiotic, metronidazole (METRO), which targets protozoan parasites of Trichomonas genus and a broad spectrum of anaerobic bacteria, e.g. gram-negative bacteria genera Bacteroides, Helicobacter, Prevotella, and gram-positive bacteria such as genus Clostridium²⁷. METRO converts into nitroso free radicals in the cytoplasm of bacteria or in specific organelles in the protozoa, which inhibits DNA synthesis and damages DNA by oxidation. The drug is only active against bacteria with anaerobic metabolisms and some microaerophils, as aerobic cells lack electron-transport proteins with sufficient negative redox potential⁴³.

As carotenoids have been associated with signalling overall parasite resistance, we hypothesized that medicating birds with either TOLTRA or METRO would result in higher plasma carotenoids and higher chroma in lab-grown feathers compared to the control group. Our study design allowed us to distinguish between the effects of treatment against Isospora coccidian parasites (affecting intestinal integrity) vs. treatment against Trichomonas parasites (affect nutrient availability but not nutrient absorption in the gut) and various anaerobic bacteria (might affect general health state). Accordingly, we predicted that if intestinal tract integrity is the key factor linking intestinal parasite infection to carotenoid coloration, treatment with either of the drugs (TOLTRA or METRO) would affect plasma carotenoid concentration. Alternatively, if the key factor is overall health state, then birds treated with METRO would show a greater increase in plumage carotenoid coloration. It should be noted however that in our study, we did not directly measure the changes in the microbial community or gut integrity, so our set-up can provide only indirect evidence to support these hypotheses.

Methods

Study system and ethics statement

The study was conducted under license from the Estonian Ministry of the Environment (Licence # 1–4.1/11/100, issued on 23rd March 2011), and the experiment was approved by the Committee of Animal Experiments at the Estonian Ministry of Agriculture (decision # 95, issued on 17th January 2012). All experiments were performed in accordance with relevant guidelines and regulations. The study was carried out in compliance with the ARRIVE guidelines. Wild male greenfinches (N = 71) were captured in mist nets at bird feeders in a garden in the city of Tartu, Estonia (58°22′N; 26°43′E) on 5th, 6th, and 8th January 2015. Greenfinches are gregarious medium-sized (c. 28 g) seed-eating passerines native to the western Palearctic region⁴⁴. Males are more colourful with yellow, carotenoid-based⁴⁵ markings on the sides of the tail feathers, primaries, primary coverts, and breast, while females lack full yellow tints in their plumage⁴⁴. Greenfinches incorporate two main carotenoids − canary xanthophylls A and B − into feathers to develop the yellow colour. Canary xanthophylls A and B are metabolically converted from dietary lutein and zeaxanthin^46,47. The birds were housed indoors in individual cages (27 × 51 × 55 cm) with sand-covered floors in a single room where they had visual contact with their neighbours. The average temperature in the aviary during the experiment was 15.5 ± 1.0° (SD) °C, and average humidity was 57 ± 7 (SD) %. The birds were supplied ad libitum with sunflower seeds and tap water, and were exposed to a natural day-length cycle using artificial lighting by luminophore tubes. The birds were released back to their natural habitat on 3rd March 2015.

We divided birds into three approximately equal-sized groups. The groups did not differ in age composition (approximately an equal number of both, yearlings vs. older birds, in every group, determined based on plumage characteristics), body mass (recorded on 19th January, F_2,68 = 0.20, p = 0.82), and coccidian infection intensity (recorded on 15th January, F_2,69 = 0.045, p = 0.96). The timeline of the study is depicted in Fig. 1. On the evening of 19th January, the birds in the two groups subjected to the medication treatment started to receive either TOLTRA (24 birds) or METRO (24 birds) with their carotenoid-enriched drinking water. Twenty-three control birds received just carotenoid-enriched water. Birds in the anticoccidial medication group received a two ml/L solution of Intracox Oral (Interchemie, Castenary, the Netherlands), containing 25 mg/L toltrazuril. METRO (Fresenius Kabi Polska, Kutno, Poland) was administered at a concentration of 400 mg/L. Both drugs were dissolved in carotenoid solution (1 ml/L mix of lutein and zeaxanthin (20:1, w/w), prepared from OroGlo brand 15 Liquid Pigmenter with 15 g/kg xanthophyll activity (Kemin AgriFoods Europe, Herentals, Belgium). The concentration of carotenoid supplement in drinking water was chosen based on previous experiments with greenfinches⁴⁸. Carotenoids were added to the drinking water to compensate for the naturally low carotenoid content of sunflower seeds. Medication lasted for ten days, and carotenoid supplementation lasted until the birds were released. We took blood samples from the birds on 19th January and 30th January to record the effects of treatments on plasma carotenoid concentrations. We weighed the birds after trapping, on 19th January, on 30th January, and before the release on 3rd March. Blood sampling of birds took place in the mornings before the lights turned on. We collected the samples for assessment of coccidian infection intensity on 15th January, on 28th January, and 3rd March. The study duration (9th January–3rd March) was based on the time it takes greenfinches to regrow plucked feathers⁴⁹.

Figure 1.

Timeline of the experiment. The dates are in DD/MM format.

Measurement of chroma

We plucked the left and right outermost wild-grown tail feathers (rectrices) on 9th January from 71 birds. Replacement feathers grown during the study (lab-grown feathers) were collected on 3rd March, before the release of the birds. We placed collected feathers into a plastic bag and stored them in the dark until measurements were carried out. Locations of colour measurements on feathers are indicated in Fig. 2. The colour was measured from the feathers placed on a black background, in an area of approximately one mm², of the visible surface of the feather, using a spectrophotometer (Ocean Optics S2000) as described by L. Saks and co-authors³⁸. To estimate colour, we calculated values of chroma⁵⁰. Chroma can be understood as a measure of the ‘purity’ or ‘saturation’ of colour and has been shown to correlate with the actual carotenoid concentration of feathers. Details for calculations of chroma are described by L. Saks and co-authors³⁸. The chroma measurements of left and right feathers were repeatable in lab-grown feathers and wild-grown feathers (r = 0.7, F_60;61 = 5.1, p < 0.0001)⁵¹.

Figure 2.

Measurement of feather chroma. The arrows indicate positions where chroma of yellow feather coloration was measured. Upper – lab-grown feather, lower – wild-grown feather.

Infection intensity and blood analyses

We collected faecal samples to determine coccidian (Isospora spp.) infection intensity from all the birds on 15th January, 28th January, and 3rd February, as described by R. Meitern and co-authors³⁴, and all birds appeared naturally infected. We have previously shown that repeatability of infection intensity, measured from two faecal samples collected at the same time, was high (0.91, F = 20.34, p < 0.0001, n = 20)⁵². Diagnosis of trichomonosis requires either sacrificing the birds or PCR-based techniques, which were not available to us during the study²⁷. Therefore, we were not able to collect data on the infection status of trichomonads for this study, but previous investigations of dead birds have confirmed that trichomonosis is present in the Estonian greenfinch population. For instance, three birds of the five that died in captivity in our lab during 2013 showed apparent symptoms of trichomonosis but the prevalence of sublethal levels of infection is not known⁴⁰. The concentration of carotenoids was determined spectrophotometrically from 15 μl of plasma, diluted in acetone as described elsewhere⁷. We assessed the nutritional state based on plasma triglycerides, as described by R. Meitern and co-authors³⁴. Plasma triglycerides increase during the transport of fat to the adipose tissue and energy-consuming organs and indicate the amount of lipids absorbed and changes in fat stores⁵³.

Statistics

We analysed the effect of experimental manipulation on feather chroma with one-way ANOVA. The assumptions of parametric tests were met (normality of residuals, homogeneity of variances). We used Tukey’s post hoc test to determine the differences between the groups. The effect of experimental manipulation on coccidian infection intensity, plasma carotenoids, plasma triglycerides, and body mass were examined with repeated measures ANOVA, assuming that the effect of treatment would be revealed by a significant ‘time × treatment’ interaction term. All tests were two-tailed, with a P-level below 0.05 as a criterion for significance. Sample sizes varied between analyses due to our inability to collect sufficient good-quality blood samples from all birds. Analyses were performed with Statistica v. 12 (Statsoft Inc., Tulsa, OK). We only examined male greenfinches as carotenoid-based signal traits are more pronounced in males in this species, and the addition of another factor (sex) would have decreased test power. Female birds were used in a different study³⁴. We also explored the correlation between chroma, infection intensity, body mass, plasma carotenoids, and plasma triglycerides. All data generated or analysed during this study are included in Supplementary Data.

Results

Treatment’s effect on feather chroma

The experimental treatment significantly affected the chroma of the yellow parts of the lab-grown tail feathers (ANOVA F_2,62 = 5.6, p = 0.006, Fig. 3). Birds that received METRO had higher chroma than those in the control group (Tukey’s post hoc, p = 0.012) and in the TOLTRA group (Tukey’s post hoc p = 0.016), whereas there was no difference in the chroma between birds treated with TOLTRA and the control group. There was no difference in the chroma in the wild-grown feathers between the experimental groups (F_2,68 = 0.43, p = 0.70) and no correlation between chroma of wild-grown and lab-grown feathers (r = 0.093, n = 65, p = 0.46; Fig. 4). Nor were there any relationships between body mass and feather chroma at any point measured (Fig. 4; Supplementary Table S1). Age of birds (juvenile vs. adult) did not affect chroma in wild-grown feathers (ANOVA F_{1, 68} = 1.31, p = 0.26) and lab-grown feathers (ANOVA F_{1, 63} = 0.073, p = 0.79).

Figure 3.

The effect of treatment on feather chroma. The treatment affected chroma of the yellow parts of the lab-grown feathers—birds that received metronidazole (METRO) medication had higher chroma than in the control group and in the toltrazuril (TOLTRA) group. Whiskers denote mean ± s.e.m.

Figure 4.

Correlations between feather chroma and physiological and phenotypical traits. Asterisks after the panel letter indicate significant relationships.

Infection intensity, plasma biomarkers, and body mass

Treatment did not affect the body mass of birds (Table 1, Fig. 5). There were correlations between body mass and triglycerides on 19th January, on 30th January (Supplementary Table S1), and between body mass at releasing the birds on 3rd March (r = 0.49, n = 55, p = 0.001; Fig. 6).

Table 1.

Effects of treatment on infection intensity, plasma carotenoid, triglycerides and body mass. See Fig. 5 for the directions of the effects. Effects on infection intensity, body mass, and plasma triglycerides have been previously reported by R. Meitern and co-authors in³⁴ (https://doi.org/10.1002/ece3.2575, licensed under CC BY 4.0 https://creativecommons.org/licenses/by/4.0/).

	Effect	Fdf	p
Infection intensity	Treatment	43.9 2,65	< 0.00001
	Time	2.5 2,130	0.087
	Time*treatment	24.5 4,130	<0.00001
Plasma carotenoids	Treatment	2.67 2,62	0.078
	Time	21.82 1,62	0.00002
	Time*treatment	5.02 2,62	0.0095
Triglycerides	Treatment	2.94 2,47	0.063
	Time	12.04 1,47	0.0011
	Time*treatment	1.71 2,47	0.19
Body mass	Treatment	0.01 2,65	0.99
	Time	78.11 2,124	<0.00001
	Time*treatment	1.58 4,124	0.18

Figure 5.

The effect of treatment on body mass, plasma carotenoids, plasma triglycerides, and coccidian infection intensity. (a) Treatment did not affect the body mass of birds. (b) Treatment had an effect on plasma carotenoids—carotenoids were significantly higher in the toltrazuril group pre-treatment, but after medication, there were no differences between the groups. (c) Treatment did not affect plasma triglycerides. (d) Medication with TOLTRA significantly reduced the intensity of coccidian infection, while birds medicated with metronidazole (METRO) did not differ from the control group (modification of a previously published figure by R. Meitern and co-authors³⁴ (https://doi.org/10.1002/ece3.2575, licensed under CC BY 4.0 https://creativecommons.org/licenses/by/4.0/). The effects were examined with repeated measures ANOVA (Table 1). Whiskers denote mean ± s.e.m.

Figure 6.

Correlations between physiological and biochemical traits. Asterisks after the panel letter indicate significant relationships.

The effects of treatment on coccidian infection intensity have been reported in a previously published article on this study by R. Meitern and co-authors³⁴. Medication with TOLTRA significantly reduced the intensity of coccidian infection while METRO had no effect and did not differ from the control group (Table 1, Fig. 5 and previously reported in³⁴). Pre-treatment infection intensity (ln-transformed oocysts count) did not correlate with chroma of wild-grown feathers (r = 0.14, n = 72, p = 0.24; Fig. 4) and post-treatment infection intensity (measured on 3rd February) did not correlate to chroma of lab-grown feathers (r = 0.14, n = 65, p = 0.25, Fig. 4). Neither were there any other relationships between infection intensity and wild-grown feather chroma or lab-grown feather chroma at any dates measured (Supplementary Table S1).

The treatment had an effect on plasma carotenoids (Table 1, Fig. 5). Plasma carotenoids declined in all groups, but the decline was steepest in the TOLTRA group. Carotenoids were significantly higher in the TOLTRA group pre-treatment (ANOVA F_2,67 = 3.73, p = 0.03), but after medication, there were no differences between groups (ANOVA F_2,63 = 2.66, p = 0.08). Effects of treatment on plasma triglycerides were previously reported by R. Meitern and co-authors³⁴. No significant effect of treatment on plasma triglycerides was detected (Table 1, Fig. 5). Plasma triglycerides on 19th and 30th January correlated positively with plasma carotenoids measured on 30th January (r = 0.24, n = 56, p = 0.048; r = 0.41, n = 58 p = 0.0013¸ Fig. 6). We also detected that chroma of lab-grown feathers and plasma carotenoids on 19th January and 30th January were correlated (r = 0.33, n = 64, p = 0.008; r = 0.42, N = 62, p = 0.0006; Fig. 4). However, there were no correlations between plasma carotenoids and chroma of wild-grown feathers on neither of the days (Supplementary Table S1).

Discussion

Carotenoid coloration is suggested to signal both parasite load and resistance of the individual. Our goal was to test whether parasites affect yellow feather coloration through decreased nutrient acquisition. We administered anticoccidial drug TOLTRA or antibiotic METRO, which targets Trichomonas protozoa and anaerobic bacteria, to wild-caught captive greenfinches. We found that the treatment with METRO resulted in significantly higher chroma of yellow parts of the feathers, whereas feather colour in birds who received anticoccidial TOLTRA did not differ from the control. There were no significant differences in plasma carotenoids nor in plasma triglycerides between the groups after the treatment, indicating that treatment eliminated prior differences in absorption or availability of these nutrients. Coccidian infection intensity significantly declined in the TOLTRA group, but there were no differences between the METRO and the control group. Treatment did not affect body mass.

We suggest that treatment with antibiotic METRO attenuated the negative effects of trichomonosis, or alternatively, the potential unknown co-infection between several pathogenic bacteria, or between trichomonas and pathogenic bacteria, which freed additional energetic resources that could be invested in ornamentation¹³. One might speculate that antibiotic METRO might have induced change in gut microbiota composition. Some metabolites of microbiota, such as short-chain fatty acids (SCFAs), can affect mitochondrial function^54,55 resulting in the improvement of vital cellular processes that also influence modification and deposition of carotenoids to the feathers¹². There was no effect of treatment on yellow feather coloration in the TOLTRA group. One possible explanation could be that birds might have been naturally infected with mildly virulent strains of coccidia that had little effect on their health before the treatment. For instance, it has been demonstrated previously in captive greenfinches that coccidian strains infecting different individuals vary largely in their virulence and hosts differ in resistance⁵².

One explanation for why birds who received METRO grew more saturated yellow feathers might be that METRO had an effect on the co-infection dynamics that freed additional energy resources which could be allocated to production of ornaments. Most individuals in the wild are faced with co-infection of several parasites or several different strains of one parasite⁵⁶, and indeed, all the greenfinches in our study were naturally infected with Isospora parasites. We suggest that the wide spectrum antibiotic METRO acted on Trichomonas gallinae and on some other (bacterial) infections unknown to us. Although we were not able to diagnose trichomonosis in birds used in this study, previous investigations of dead birds have confirmed that trichomonosis is present in Estonian greenfinch population⁴⁰. The prevalence of Trichomonas parasites has been measured in the greenfinch population in Hesse, Germany, where it showed a high prevalence with 25% of the population being infected and the highly pathogenic strain was widespread⁵⁷. Co-infection can affect the virulence of the parasites⁵⁸, but also the host’s immune responses⁵⁶. Israeli sparrows (Passer domesticus biblicus) that were simultaneously infected by Isospora and Leucocytozoon gentili experienced more severe symptoms of coccidiosis⁵⁹. Carotenoid feather coloration was paler in blue tits (Cyanistes caeruleus) who were infected by different numbers of blood parasite genera compared to those infected by just one¹³. Necrotic enteritis in poultry has been shown to occur when birds with pre-existing mild or asymptomatic coccidiosis are infected with pathogenic Clostridium spp. strains (reviewed by^60,61). Members of Clostridium genus are sensitive to METRO, as are many other anaerobic bacteria⁶². It is possible that by administering METRO, the birds were relieved from the adverse effects of co-infection between coccidia and pathogenic bacteria. However, we did not measure microbiome and for definitive evidence this should be tested in future studies. In conclusion, allocating energy between fighting multiple infections and other physiological functions can result in duller ornaments because carotenoid uptake, transportation, modification, and deposition to ornaments is energetically demanding in itself⁶³. Thus, carotenoids might signal energetic state of the animal.

We suggest that the effect of METRO on yellow feather coloration might have resulted from improved vital cellular processes that are associated with carotenoid modification and carotenoid deposition into feathers¹². It has been proposed that the modification of carotenoids is related to the capacity to produce energy in the membranes of mitochondria⁶⁴. Antibiotics can affect host energy metabolism through altering gut microbiota composition. In addition to antiparasitic effects, antibiotics can be harmful as they can alter the normal microflora. However, recent research has found that some antibiotics can increase the abundance of beneficial bacteria (reviewed by⁶⁵). These bacteria can modulate the intestinal immune system and are important producers of short-chain fatty acids (SCFAs), which act as regulatory molecules that influence energy homeostasis and mitochondrial function (reviewed by^54,55). Due to their common ancestry, mitochondria share several structural and functional features with prokaryotes, hence many bacterial metabolites (such as SCFAs, secondary bile acids, lipopolysaccharides, urolithins) have been shown to both affect mitochondria and have other regulatory and immunomodulatory effects (reviewed by^54,55,66). It is possible that although METRO treatment did not result in higher nutrient absorption, it might have elicited a shift in gut microbiota composition that improved the host’s cellular processes. Our results are consistent with the shared pathway hypothesis; however, future experimental work into connections between mitochondrial function, ornamentation, and microbiota is needed.

Treatment with TOLTRA significantly reduced coccidian infection intensity but did not affect yellow feather-chroma in lab-grown feathers. Coccidian infection intensity did not correlate to either wild-grown or lab-grown feather coloration at any date measured. The lack of any effect from treatment with TOLTRA and coccidian infection intensity on feather colour might have been due to low virulence of natural infection and that the strains of coccidia were familiar to the birds; greenfinches have better tolerance to their ‘own’ previously acquired parasites than to novel strains⁵². Infection intensities were higher when birds were infected with strains that originated from several hosts, opposed to when birds were inoculated with coccidia strains from one single host⁵², which indicates a variation in virulence in Isospora coccidia in greenfinches. We suggest that the naturally occurring coccidian infection did not cause considerable intestinal damage in our study system that would have had any effect on the absorption of carotenoids. It has been suggested that birds in the wild can tolerate Isosporan parasites well but can experience more serious illness under stressful conditions (e.g. captivity), poor sanitary conditions, or with concurrent infections^24,59. Greenfinches, however, have previously been shown to tolerate captivity well based on low stress hormone levels in captivity⁶⁷ and several haematological indices⁶⁸. Thus, the stress of captivity might not have been severe enough to induce exacerbation of the coccidian infection.

The effect of treatment on plasma carotenoids and triglycerides suggests that there were no differences in carotenoid and triglyceride absorption between groups after the treatment. Plasma carotenoid levels were significantly higher before the treatment in birds who subsequently received TOLTRA. Plasma characteristics from blood were measured after the experiment was carried out and could not be considered while grouping the birds; thus, pre-treatment carotenoids in the TOLTRA group are higher by chance. After the treatment plasma carotenoids did not differ between groups; however, plasma carotenoid levels declined in all groups. We suggest that carotenoid levels in all the birds fell to approximately the same levels in the lab conditions because all birds received a constant amount of carotenoids. Negative effect of TOLTRA on plasma carotenoid levels cannot be ruled out, however, we are not aware of any such findings. It is thus more likely that equal carotenoid availability in the diet during captivity was responsible for uniform plasma carotenoid levels in all our treatment groups. Similar decrease in plasma carotenoid levels during captivity has been recorded in our previous experiments with greenfinches^7,14,68. Across all treatment groups, plasma carotenoids on both blood-sampling dates were positively correlated with yellow feather chroma in lab-grown feathers (but not with wild-grown feathers). However, similar post-treatment carotenoid plasma levels support our suggestion that more saturated yellow lab-grown feathers in the METRO group were not only related to the plasma carotenoid levels, but also might have involved a component of increased efficiency in carotenoid modification or deposition.

Opposed to the effects of antibiotics in poultry, treatment did not affect the body mass in greenfinches. In poultry studies, improved body mass gain with antibiotic growth promoters is usually observed during the period between post-hatch and maturation. The birds in our study were either adults or yearlings who had reached their adult weight by the time of the study. Therefore, in our study system, the effect of treatment on feather coloration was independent of body mass and the body weight of birds might have depended more on other factors rather than mild to moderate infection. We did not find significant effect of treatment on plasma triglycerides. However, there was positive correlation between triglycerides and body mass on 19th January, 30th January and 3rd March. Carotenoids on 30th January (but not on 19th January) positively correlated with both measurements on triglycerides. Although there are relations between body weight and triglycerides, as well as between triglycerides and plasma carotenoids, these do not reflect directly in the carotenoid coloration, based on the results of this study.

To conclude, treatment of captive greenfinches with two antimicrobial agents – narrow-spectrum anticoccidial Toltrazuril and a wide spectrum antibacterial/anti-trichomonad Metronidazole did not affect plasma carotenoid levels, indicating that neither of our treatments affected carotenoid absorption. Among the birds treated with metronidazole, feathers grown during the experiment had significantly higher yellow chroma, which is indicative of higher deposition of dietary carotenoids into feathers. The latter finding provides indirect support to the hypothesis that the microbiome is important for carotenoid metabolism, transportation, and/or deposition. Assuming that microbial metabolites can modulate mitochondrial and immune function by altering the efficiency of vital cellular processes, our findings are also consistent with the shared pathway hypothesis whereby the mechanisms of production of ornaments share functional pathways with core-life supporting pathways. Specific mechanisms into how the microbiome relates to carotenoid metabolism and signalling thus await further investigation.

Author contributions

P.H. and M.-A.L. provided the original idea for the study; P.H., T.S. designed and managed the project; K.Š., T.S., M.-A.L. performed the experiments and collected the data; M.-A.L. and K.Š analysed the data; M.A.L drafted the manuscript, prepared the figures and took the photographs; T.S, P.H. reviewed the manuscript. All authors read and approved the final version of the manuscript.

Funding

The study was financed by the Estonian Research Council (Grants IUT34-8, PUT653, PSG458 and PRG1137).

Data availability

All data generated or analysed during this study are included in Supplementary Information.

Competing interests

The authors declare no competing interests.

Supplementary Information

The online version contains supplementary material available at 10.1038/s41598-021-92598-x.