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. 2010 Feb 12;25(1):18–26. doi: 10.1007/s12250-010-3041-4

B cell epitopes within VP1 of type O foot-and-mouth disease virus for detection of viral antibodies

Shan-dian Gao 1, Jun-zheng Du 1, Hui-yun Chang 1,✉, Guo-zheng Cong 1, Jun-jun Shao 1, Tong Lin 1, Shuai Song 1, Qing-ge Xie 1
PMCID: PMC8227897  PMID: 20960280

Abstract

In this study, the coding region of type O FMDV capsid protein VP1 and a series of codon optimized DNA sequences coding for VP1 amino acid residues 141–160 (epitope1), tandem repeat 200–213 (epitope2 (+2)) and the combination of two epitopes (epitope1–2) was genetically cloned into the prokaryotic expression vector pPROExHTb and pGEX4T-1, respectively. VP1 and the fused epitopes GST-E1, GST-E2 (+2) and GST-E1-2 were successfully solubly expressed in the cytoplasm of Escherichia coli and Western blot analysis demonstrated they retained antigenicity. Indirect VP1-ELISA and epitope ELISAs were subsequently developed to screen a panel of 80 field pig sera using LPB-ELISA as a standard test. For VP1-ELISA and all the epitope ELISAs, there were clear distinctions between the FMDV-positive and the FMDV-negative samples. Cross-reactions with pig sera positive to the viruses of swine vesicular disease virus that produce clinically indistinguishable syndromes in pigs or guinea pig antisera to FMDV strains of type A, C and Asia1 did not occur. The relative sensitivity and specificity for the GST-E1 ELISA, GST-E2 (+2), GST-E1-2 ELISA and VP1-ELISA in comparison with LPB-ELISA were 93.3% and 85.0%, 95.0% and 90%, 100% and 81.8%, 96.6% and 80.9% respectively. This study shows the potential use of the aforementioned epitopes as alternatives to the complex antigens used in current detection for antibody to FMDV structural proteins.

Key words: Foot-and-mouth disease virus (FMDV), Serology, Epitope ELISA

Footnotes

Foundation item: National key Technology R&D Program (2006BAD06A14).

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Articles from Virologica Sinica are provided here courtesy of Wuhan Institute of Virology, Chinese Academy of Sciences

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