Abstract
The complete nucleotide sequence of the measles virus strain IMB-1, which was isolated in China, was determined. As in other measles viruses, its genome is 15,894 nucleotides in length and encodes six proteins. The full-length nucleotide sequence of the IMB-1 isolate differed from vaccine strains (including wild-type Edmonston strain) by 4%–5% at the nucleotide sequence level. This isolate has amino acid variations over the full genome, including in the hemagglutinin and fusion genes. This report is the first to describe the full-length genome of a genotype H1 strain and provide an overview of the diversity of genetic characteristics of a circulating measles virus.
Key words: Complete nucleotide sequence, Genotype H1, Measles virus (MV)
Footnotes
The authors contributed equally to this work.
Fundation items: Public Benefit Grant of Ministry of Health P.R China (200802035) and Basic Research Foundation (General Program) of Yunnan Province (2008CD153)
References
- 1.Bellini W. J., Rota J. S., Rota P. A. Virology of measles virus. J Infect Dis. 1994;170.(Suppl1):S15–23. doi: 10.1093/infdis/170.supplement_1.s15. [DOI] [PubMed] [Google Scholar]
- 2.Curtale F., Perrelli F., Mantovani J., et al. Description of two measles outbreaks in the Lazio Region, Italy (2006–2007). Importance of pockets of low vaccine coverage in sustaining the infection. BMC Infect Dis. 2010;10:62. doi: 10.1186/1471-2334-10-62. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 3.de Swart R. L., Yüksel S., Langerijs C. N., et al. Depletion of measles virus glycoprotein-specific antibodies from human sera reveals genotype specific neutralizing antibodies. J Genel Virol. 2009;90:2982–2989. doi: 10.1099/vir.0.014944-0. [DOI] [PubMed] [Google Scholar]
- 4.Hashiguchi T., Kajikawa M., Maita N., et al. Crystal structure of measles virus hemagglutinin provides insight into effective vaccines. Proc Natl Acad Sci USA. 2007;104:19535–19540. doi: 10.1073/pnas.0707830104. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 5.Hutchins S. S., Papania M. J., Amler R., et al. Valuation of the measles clinical case definition. J Infect Dis. 2004;189(Suppl1):S153–159. doi: 10.1086/379652. [DOI] [PubMed] [Google Scholar]
- 6.Ji Y., Zhang Y., Xu S., et al. Measles resurgence associated with continued circulation of genotype H1 viruses in China, 2005. Virol J. 2009;6:135–143. doi: 10.1186/1743-422X-6-135. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 7.Kerdiles Y. M., Sellin C. I., Druelle J., et al. Immunosuppression caused by measles virus: role of viral proteins. Rev Med Virol. 2006;16:49–63. doi: 10.1002/rmv.486. [DOI] [PubMed] [Google Scholar]
- 8.Kouadio I. K., Kamigaki T., Oshitani H. Measles outbreaks in displaced populations: a review of transmission, morbidity and mortality associated factors. BMC Int Health Hum Rights. 2010;10:5. doi: 10.1186/1472-698X-10-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 9.Liebert U. G., Flanagan S. G., Löffler S., et al. Antigenic determinants of measles virus hemagglutinin associated with neurovirulence. J. Virol. 1994;86:1486–1493. doi: 10.1128/jvi.68.3.1486-1493.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 10.Masse N., Ainouze M., Neel B., et al. Langedijk JP. Measles virus (MV) hemagglutinin: evidence that attachment sites for MV receptors SLAM and CD46 overlap on the globular head. J Virol. 2004;78:9051–9063. doi: 10.1128/JVI.78.17.9051-9063.2004. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 11.Moss W. J., Griffin D. E. Global measles elimination. Nat Rev Microbiol. 2006;4:900–908. doi: 10.1038/nrmicro1550. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 12.Okada H., Itoh M., Nagata K., et al. Previously unrecognized amino acid substitutions in the hemagglutinin and fusion proteins of measles virus modulate cell-cell fusion, hemadsorption, virus growth, and penetration rate. J Virol. 2009;83:8713–8721. doi: 10.1128/JVI.00741-09. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 13.Parks C. L., Lerch R. A., Walpita P., et al. Comparison of predicted amino acid sequences of measles virus strains in the Edmonston vaccine lineage. J Virol. 2001;75:910–920. doi: 10.1128/JVI.75.2.910-920.2001. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 14.Rall G. F., Manchester M., Daniels L. R., et al. A transgenic mouse model for measlesvirus infection of the brain. Proc Natl Acad Sci USA. 1997;94:4659–4663. doi: 10.1073/pnas.94.9.4659. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 15.Rota P. A., Featherstone D. A., Bellini W. J. Molecular epidemiology of measles virus. Curr Top Microbiol Immunol. 2009;330:129–150. doi: 10.1007/978-3-540-70617-5_7. [DOI] [PubMed] [Google Scholar]
- 16.Sugerman D. E., Barskey A. E., Delea M. G., et al. Measles outbreak in a highly vaccinated population, san diego, 2008: role of the intentionally undervaccinated. Pediatrics. 2010;125:747–755. doi: 10.1542/peds.2009-1653. [DOI] [PubMed] [Google Scholar]
- 17.Tahara M., Takeda M., Seki F., et al. Multiple amino acid substitutions in hemagglutinin are necessary for wild-type measles virus to acquire the ability to use receptor CD46 efficiently. J Virol. 2007;81:2564–2572. doi: 10.1128/JVI.02449-06. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 18.Vongpunsawad S., Oezgun N., Braun W., et al. Selectively receptor-blind measles viruses: identification of residues necessary for SLAM- or CD46-induced fusion and their localization on a new hemagglutinin structural model. J Virol. 2004;78:302–313. doi: 10.1128/JVI.78.1.302-313.2004. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 19.Zarocostas J. Measles deaths fell by more than 90% worldwide from 2000 to 2008, except in southern Asia. BMJ. 2009;339(8):1337. doi: 10.1136/bmj.b5362. [DOI] [PubMed] [Google Scholar]
- 20.Zhang Y., Ji Y., Jiang X., et al. Genetic characterization of measles viruses in China, 2004. Virol J. 2008;5:120–126. doi: 10.1186/1743-422X-5-120. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 21.Zhang Y., Ji Y. X., Zhu Z. Circulating pattern analysis for endemic measles viruses in mainland of China. Chin J Vaccin Immun. 2009;15:97–103. [PubMed] [Google Scholar]