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Virologica Sinica logoLink to Virologica Sinica
. 2010 Apr 9;25(2):123–129. doi: 10.1007/s12250-010-3115-3

Molecular characterization of nonstructural protein NS38 of grass carp reovirus

Ling Shao 1,2, Chao Fan 1,2, Ewa Maj 1, Qin Fang 1,
PMCID: PMC8227927  PMID: 20960309

Abstract

Viral nonstructural proteins in both enveloped and non-enveloped viruses play important roles in viral replication. Protein NS38 of Grass carp reovirus (GCRV), has been deduced to be a non-structural protein, and, consistent with other reoviruses, is considered to cooperate with the NS80 protein in viral particle assembly. To investigate the molecular basis of the role of NS38, a complete protein was expressed in E.coli for the first time. It was found that there is a better expression of NS38 induced with IPTG at 28 °C rather than 37 °C. In addition, the antiserum of NS38 prepared with purified fusion protein and injected into rabbit could be used for detecting NS38 protein expression in GCRV infected cell lysate, while there is not any reaction crossed with purified virus particle, confirming NS38 is not a component of the viral structural protein. The result reported in this study will provide evidence for further viral protein-protein and protein-RNA interaction in dsRNA viruses replication.

Key words: Grass carp reovirus (GCRV), Nonstructural protein NS38, Recombinant expression

Footnotes

Foundation items: National Basic Research Program (973) of China ( 2009CB118701); National Natural Scientific Foundation of China (30871940, 30671615).

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