Table 1.
Composition of the internal standard mixture. A volume of 50 µL was added per sample.
| Species | Molecular Weight | Stock Solution | Spiked Amount | |
|---|---|---|---|---|
| (g/mol) | (µg/mL) | (ng/sample) | (nmol/sample) | |
| CE 17:0 | 638.60 | 10 | 500 | 0.78 |
| CE 22:0 | 708.68 | 10 | 500 | 0.71 |
| Cer 32:1;O2 | 509.48 | 1 | 50 | 0.098 |
| Cer 35:1;O2 | 551.53 | 1 | 50 | 0.091 |
| DG 28:0 | 512.44 | 5 | 250 | 0.49 |
| DG 40:0 | 680.63 | 5 | 250 | 0.37 |
| [D7]FC | 393.40 | 75 | 3750 | 9.5 |
| LPC 13:0 | 453.29 | 1 | 50 | 0.11 |
| LPC 19:0 | 537.38 | 1 | 50 | 0.093 |
| LPE 13:0 | 411.24 | 1 | 50 | 0.12 |
| PC 28:0 | 677.50 | 25 | 1250 | 1.8 |
| PC 44:0 | 901.75 | 25 | 1250 | 1.4 |
| PE 28:0 | 635.45 | 10 | 500 | 0.79 |
| PE 40:0 | 803.64 | 10 | 500 | 0.62 |
| SM 30:1;O2 | 646.50 | 10 | 500 | 0.77 |
| TG 51:0 | 848.78 | 18 | 900 | 1.1 |
| TG 57:0 | 932.88 | 18 | 900 | 0.96 |