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. 2021 Jun 4;11(6):837. doi: 10.3390/biom11060837

Figure 5.

Figure 5

Immunophenotype of M2 macrophages obtained from CFA and EAE mice. Monocytes obtained from spleen of CFA and EAE mice were differentiated into macrophages using M-CSF (50 ng/mL) for 6 days and then polarized into M2 with IL-4 (20 ng/mL) for 2 more days. Expression of markers by flow cytometry upon staining cells at cell surface (CD40, MHC-II, CD80, CD86, CD206, CD11c, and CD44) or intracellularly (iNOS). Data are reported as percentage of positive cells and are representative of eight independent experiments ± SEM. * p < 0.05, ** p < 0.01.