Table 4.
Extracellular metabolites released from B. megaterium TG1-E1. The ratios show metabolite levels from TG1-E1 with hyperosmotic stress (by PEG30) compared to the non-stressed condition. N = 3 biological replicates. All ratios have p < 0.05, Student’s t-test. MeOX: methoximated-derived compound.
| Compound | Mock | PEG30 | LogFC |
|---|---|---|---|
| Piruvate | 0.04 | 0.14 | 1.72 |
| beta-Aminoisobutiric acid | 3.99 | 11.99 | 1.59 |
| acid α-ketocaproate | 0.35 | 2.88 | 3.06 |
| Urea | 20.53 | 55.66 | 1.44 |
| Ethanolamine | 2.11 | 15.58 | 2.88 |
| Leucine | 2666.87 | 6457.33 | 1.28 |
| Succinate | 51.39 | 130.40 | 1.34 |
| Glycerate | 6.04 | 16.87 | 1.48 |
| Fumarate | 4.64 | 9.08 | 0.97 |
| Serine | 7.56 | 34.32 | 2.18 |
| Threonine | 8.33 | 50.54 | 2.60 |
| beta-Alanine | 1.19 | 17.05 | 3.84 |
| Glutamine | 1.51 | 6.59 | 2.13 |
| Malate | 2.20 | 5.55 | 1.33 |
| Cytosine | 0.05 | 0.17 | 1.72 |
| L-Aspartate | 48.05 | 160.37 | 1.74 |
| Ornithin-Citrullin-Arginine | 1.09 | 4.55 | 2.06 |
| Xylose MeOX1 | 0.12 | 1.55 | 3.65 |
| Ribose | 0.22 | 0.64 | 1.54 |
| cis-Aconitate | 0.18 | 0.56 | 1.65 |
| 1-Methyl-L-histidine | 0.11 | 0.49 | 2.19 |
| Glycerate-3-P | 0.40 | 0.97 | 1.28 |
| Citrullin-Ornithin-Arginine | 6.14 | 78.11 | 3.67 |
| Citrate | 2.13 | 6.83 | 1.68 |
| Isocitrate | 0.52 | 1.45 | 1.47 |
| Arginine-NH3 | 8.62 | 16.92 | 0.97 |
| Pinitol | 0.10 | 0.28 | 1.50 |
| Fructose MEOX1 | 0.09 | 0.30 | 1.78 |
| Fructose MEOX2 | 0.04 | 0.12 | 1.52 |
| Galactose MeOX1 | 0.11 | 0.38 | 1.84 |
| Glucose MEOX1 | 0.46 | 1.46 | 1.67 |
| Glucose MEOX2 | 0.04 | 0.17 | 1.99 |
| Glucuronic acid MEOX1 | 0.03 | 0.13 | 2.04 |
| Gluconate | 0.23 | 0.59 | 1.35 |
| myo-Inositol | 7.21 | 24.71 | 1.78 |
| Spermidine | 0.45 | 3.41 | 2.93 |
| L-Cystathionine | 0.71 | 3.80 | 2.42 |
| Tryptophan | 112.05 | 222.43 | 0.99 |
| Fructose-6-P | 0.04 | 0.09 | 1.18 |
| Gluconate-6-P | 0.03 | 0.13 | 2.17 |
| Sucrose | 0.59 | 2.00 | 1.75 |
| Trehalose | 0.33 | 0.66 | 0.98 |
| Lysine | 4737.93 | 12276.77 | 1.37 |
| Histidine | 193.44 | 393.00 | 1.02 |
| Maleic acid | 9.10 | 2.61 | −1.80 |
| Uracil | 51.66 | 13.95 | −1.89 |
| Thymine | 6.94 | 0.16 | −5.46 |
| 4-Aminobutyrate (GABA) | 20.49 | 3.28 | −2.64 |