Table 7.
Studies in multiple matrices.
| Study ID | Study Outcome |
Study Design |
Subjects | Sample Matrix |
Sample Preparation Method |
Analytical Techniques |
Data Processing Software |
Statistical Analysis Software |
Results |
|---|---|---|---|---|---|---|---|---|---|
| Dutkiewicz 2020 [33] |
Ps activity | Untargeted | 17 Ps | Skin Plasma |
Skin: Hydrogel micropatches Plasma: PPt |
Skin: nano-DESI-MS Plasma: LC-MS |
Compass DataAnalysis Custom C# |
OriginPro | Choline levels in lesional skin correlated positively with severity of the lesions, while citrulline correlated negatively. Plasma choline levels also correlated positively with the severity of the disease (PASI), while citrulline also correlated negatively. Choline and citrulline in skin and blood showed dynamic changes corresponding to resolution of Ps due to the treatment with biologics. |
| Sorokin 2018a [29] |
Ps diagnosis | Untargeted Targeted |
Plasma: 60 Ps 30 HC Skin & serum: 8 Ps 7 HC |
Plasma Skin Serum |
PPt SPE |
LC-MS/MS GC-MS |
N/A | Stata/IC | Higher arachidonic acid metabolites, as 8-, 12- and 15-hydroxyeicosatetraenoic acid, in lesional skin compared to nonlesional and skin from HC. 13-hydroxyoctadecadienoic acid increased in lesional skin compared with HC skin. Decreased antioxidant markers of glutathione and g-glutamyl and primary and secondary bile acids in the plasma of Ps patients compared to HC. |
| Sorokin 2018b [30] |
Ps diagnosis | Targeted | 7 Ps 7 HC |
Plasma Serum Skin |
PPt SPE |
LC-MS/MS | N/A | Prism Stata/IC SIMCA-P |
Increased levels of several metabolites in the EPA, DHA and AA metabolome of Ps skin. RvD5, PDx and aspirin-triggered (AT) forms of lipoxin (LX) were present only in lesional Ps skin whereas protectin D1 was present in non-lesional Ps skin. Expression of EPA, DHA and AA pathway markers in the detected peripheral blood metabolome had a similar trend as for Ps skin with slight difference for HC. |
Abbreviations: Ps, psoriasis; HC, healthy controls; PPt, protein precipitation; SPE, solid-phase extraction; LC-MS, liquid chromatography-mass spectrometry; LC-MS/MS, liquid chromatography-tandem mass spectrometry; GC-MS, gas chromatography-mass spectrometry.