Figure 6.

Effect of single-dose and repeated-dose minocycline combined with ambroxol or duloxetine on pain threshold in oxaliplatin-treated mice. Effect of minocycline (50 mg/kg, i.p.) combined with ambroxol (90 mg/kg, i.p.) on cold hyperalgesia measured in the cold plate test on the day of oxaliplatin administration (A) and 7 days later (B). Effect of minocycline (50 mg/kg, i.p.) combined with duloxetine (10 mg/kg, i.p.) on cold hyperalgesia measured in the cold plate test on the day of oxaliplatin administration (C) and 7 days later (D). The results for minocycline used in combination and minocycline used alone (50 mg/kg) are shown as the mean latency to pain reaction ± SEM for n = 9–10. Statistical analysis: repeated measures ANOVA followed by Tukey’s post-hoc comparison. Significance: ## p < 0.01, ### p < 0.001, #### p < 0.0001 vs. before oxaliplatin. Abbreviations: oxa—oxaliplatin, MI—minocycline, AM—ambroxol, DL—duloxetine. In the figure titles ‘early phase’ (A,C) and ‘late phase’ (B,D) mean two time points at which the measurements were taken (day of oxaliplatin administration and day 7 after a single-dose oxaliplatin administration, respectively). Numerical values of the latencies to pain reaction ± SEM presented in this figure are shown in the Supplementary Materials (Table S3).