Table 5.

Sequence and product lengths of target gene primers used to detect SFG and TG DNA (citrate synthase) and human β -actin DNA (internal reference gene to verify DNA quality) in the whole blood and serum DNA extracts of Australian wildlife rehabilitators participating in a survey at the Australian Wildlife Rehabilitation Conference in Sydney in July 2018.

Target Gene and Primers	Primer Sequences (5′-3′)	Product Length (bp)	Final Concentration (nM)	Reference/ Primer Source
Citrate synthase Forward primer Reverse primer Probe	TCGCAAATGTTCACGGTACTTT TCGTGCATTTCTTTCCATTGTG FAM a- TGCAATAGCAAGAACCGTAGGCTGGATG -BHQ1 b	74	300 300 200	Adapted from [47]
Human β-actin Forward primer Reverse primer Probe	CATGCCATCCTGCGTCTGGA CCGTGGCCATCTCTTGCTCG FAM a- CGGGAAATCGTGCGTGACATTAAG-BHQ1 b	172	300 300 200	Adapted From [75]

^a 6-Carboxyfluorescein, ^b Black Hole Quencher-1, SFG—Spotted fever group, TG—Typhus Group.