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. 2021 Jun 13;10(6):1489. doi: 10.3390/cells10061489

Figure 5.

Figure 5

The HSP90 inhibitor, AUY-922, modulates HCl-induced cytoskeletal rearrangements and promotes the expression of adherent junction proteins in HLMVEC. (A) The activation of RhoA induced by HCl was completely inhibited after 24 h incubation of HLMVEC with AUY-922 (2 µM). (B, C) AUY-922 inhibited MLC2 activation and increased the expression of VE-cadherin. HLMVEC grown in 100 mm cultured dishes were pre-treated for 24 h with vehicle or 2 µM AUY-922 and then exposed to 0.01 N HCl. One hour later, cells were lysed, and samples were prepared for protein analysis by Western blotting. Means ± SEM; n = 3; **: p < 0.01; ***: p < 0.001 with one-way ANOVA and Tukey’s.