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. 2021 Jun 14;14(12):3292. doi: 10.3390/ma14123292

Structural, Thermal, and Vibrational Properties of N,N-Dimethylglycine–Chloranilic Acid—A New Co-Crystal Based on an Aliphatic Amino Acid

Joanna Hetmańczyk 1,*, Łukasz Hetmańczyk 1, Joanna Nowicka-Scheibe 2, Andrzej Pawlukojć 3,4, Jan K Maurin 5,6, Wojciech Schilf 7
Editors: Jens-Uwe Sommer, Daniela Kovacheva
PMCID: PMC8232199  PMID: 34198707

Abstract

The new complex of N,N-Dimethylglycine (DMG) with chloranilic acid (CLA) was synthesized and examined for thermal, structural, and dynamical properties. The structure of the reaction product between DMG and CLA was investigated in a deuterated dimethyl sulfoxide (DMSO-d6) solution and in the solid state by Nuclear Magnetic Resonance (NMR) (Cross Polarization Magic Angle Spinning-CPMAS NMR). The formation of the 1:1 complex of CLA and DMG in the DMSO solution was also confirmed by diffusion measurement. X-ray single crystal diffraction results revealed that the N,N-dimethylglycine–chloranilic acid (DMG+–CLA) complex crystallizes in the centrosymmetric triclinic P-1 space group. The X-ray diffraction and NMR spectroscopy show the presence of the protonated form of N,N-dimethylglycine and the deprotonated form of chloranilic acid molecules. The vibrational properties of the co-crystal were investigated by the use of neutron (INS), infrared (IR), and Raman (RS) spectroscopies, as well as the density functional theory (DFT) with periodic boundary conditions. From the band shape analysis of the N–CH3 bending vibration, we can conclude that the CH3 groups perform fast (τR ≈ 10−11 to 10‒13 s) reorientational motions down to a temperature of 140 K, with activation energy at ca. 6.7 kJ mol−1. X-ray diffraction and IR investigations confirm the presence of a strong N+–H···O hydrogen bond in the studied co-crystal.

Keywords: N,N-dimethylglycine–chloranilic acid (DMG+–CLA) co-crystal; X-ray diffraction; inelastic neutron scattering (INS); temperature dependent IR spectroscopy; DFT calculation

1. Introduction

For almost 50 years N,N-Dimethylglycine (DMG) has been known as a widely used nutritional supplement in health fields, especially as a key ingredient of vitamin B-15 and as a product of the metabolism pathway of choline and methionine [1,2,3]. DMG, according to a lot of research, could positively influence the immune response in laboratory animals and humans [4], boost physical and mental performance, and enhance cardiovascular function in clinical patients. For this reason, DMG is a well-known element of supplements and medicaments and is recognized for protecting the liver by aiding in detoxification. Previously completed works are increasing our understanding of the prophylactic and therapeutic uses of DMG as a metabolic enhancer and immunomodulator. N,N-Dimethylglycine is a tertiary amino acid, the dimethylated derivative of glycine (Gly) forms molecular salts and adducts with various inorganic and organic acids [5,6,7,8], which reveal interesting physical properties leading to ferroelectric, antiferroelectric, and ferroelastic phases [9]. For several years, the salts of dimethylglycine with various inorganic and organic acids have been objects of intensive analysis [9,10,11]. Clarke et al. synthesized Dimethylglycine and its first salt dimethylglycine hydrochloride in 1933 [12]. Rodrigues et al. obtained the trifluoroacetate of dimethylglycine [13]. Anioła et al. studied the reaction of glycine, sarcosine, dimethylglycine, and betaine with squaric acid [2]. Hence, we aimed to obtain an N,N-Dimethylglycine complex with chloranilic acid (CLA). Since amino acids and their complexes are becoming increasingly popular, we proceeded with long-term research on chloranilic acid complexes with several amino acids, such as L-leucine and D-leucine [14,15]. Chloranilic acid as a significant proton donor shows simultaneously marked electron affinity as a quinoid system. CLA forms supramolecular phenomena possessing two equivalent OH groups [16,17]. The proton transfer process is still not well-understood despite the fact that it is considered as one of the most common transformations in nature. Chloranilic acid and amino acids form complexes with the proton transfer. Due to their dipolar nature caused by the presence of carboxylate anion COO and protonated amino NH3+ groups, as well as their large hyperpolarizability and chiral α carbon atom, most of the amino acids exhibit physical and chemical properties, which make them suitable material for the production of nonlinear optical (NLO) application devices. Additionally, this makes amino acids and their salts objects of diversified and intensive studies.

The vibrational properties of N,N-Dimethylglycine (DMG) were characterized thoroughly by using IR, inelastic neutron scattering (INS), and NMR spectroscopy [18,19,20,21,22]. The dynamical properties of chloranilic acid, which is simultaneously a strong proton donor and an electron acceptor, were analyzed using experimental (IR, Raman, and INS) and theoretical (DFT) methods [23].

From the point of view of materials science, information on the crystal structures of the complex between the N,N-dimethyl derivative of glycine and chloranilic acid is essential for understanding the role of hydrogen bonds and other intermolecular forces in the association of ions in the crystalline state. A new complex is characterized by X-ray diffraction. Inelastic neutron scattering (INS), infrared (IR), Raman (RS), and 1H, 13C and 15N NMR spectroscopy methods were used to determine the dynamic structure of the N,N-Dimethylglycine–chloranilic acid complex (DMG+–CLA). The structure of the complexation product of chloranilic acid (CLA) and N,N-dimethylglycine (DMG) was investigated in both a DMSO solution and in the solid state by NMR (CPMAS NMR). Additionally, DFT calculation with periodic boundary conditions for an experimental data analysis was used.

2. Materials and Methods

The substrates (N,N-Dimethylglycine and chloranilic acid) were purchased from Merck (formerly Sigma Aldrich, Merck Sp. z o.o., an affiliate of Merck KGaA, Darmstadt, Germany). They were used as delivered, without any purification. The resultant sample was obtained by mixing an equimolar (1:1) ratio of N,N-Dimethylglycine (1.03 g), which was dissolved in double distilled water, with chloranilic acid (2.08 g) dissolved in acetone. The chloranilic acid was added dropwise to DMG. Next, the obtained mixture was stirred well for about 2–3 h at 50 °C until a homogeneous solution was formed. Finally, the solution was filtered and kept undisturbed at ambient temperature. After a few weeks, the crystalline material was collected and recrystallized from a mixture of double distilled water and ethanol (1:4 ratio).

The crystal structure of the N,N-dimethylglycine–chloranilic acid co-crystal was solved from single crystal X-ray diffraction measurements. The color of the sample was dark violet and the crystals were needle-shaped. The data were collected at room temperature using a Xcalibur R diffractometer (Oxford Diffraction, Oxford, UK). The characteristic CuKα monochromatic radiation and Ruby ccd detector (Oxford Diffraction, Oxford, UK) were employed in the data acquisition. The CrysAlisPro software [24] was used for data collection strategy, data reduction, and lattice constant refinement. Structure was solved using the SHELXS-97 software (University of Göttingen, Göttingen, Germany) and refined using the SHELXL-97 program [25]. All H atoms were located using difference Fourier maps and refined freely. Their isotropic displacement parameters were tied to the respective Ueq values for the nitrogen atoms and equal to 1.2 times Ueq.

The nitrogen and carbon solid-state spectra were measured using the Bruker 500 Avance II spectrometer (Bruker BioSpin GmbH, Rheinstetten, Germany) at room temperature. All CPMAS spectra were collected using a 4 mm MAS H/BB Bruker probehead. The typical acquisition parameters for carbon CPMAS spectra were as follows: spectral width 31.25 kHz, acquisition time 20 ms, contact time 2 ms, rotation rate 10 kHz, and relaxation delay 10 s. About 100 scans were enough to obtain a good signal-to-noise ratio. For short contact time (SCT) experiments, the contact time was reduced to 40 µs. For nitrogen-15 CPMAS experiments, the following acquisition parameters were applied: spectral width 25 kHz, acquisition time 20 ms, contact time 4 ms, rotation rate 6 kHz, and relaxation delay was optimized to obtain the best signal-to-noise ratio. For some samples, up to 3000 scans were collected to obtain a sufficient S/N ratio. The glycine sample was used as the chemical shift standard material and the obtained chemical shift values were recalculated to nitromethane and TMS (tetramethylsilane) scales for nitrogen and carbon measurements, respectively. The cross polarization (CP) sequence was performed using a ramped proton pulse and continuous wave heteroatom irradiation. The modulated TPPM-15 decoupling was used during the acquisition period. All solution-state NMR measurements were run on Varian-Agilent 600 VNMRS and Varian-Agilent 500 VNMRS spectrometers (Agilent Technologies, Inc., Santa Clara, CA, United States). For the 1D carbon spectrum, a 500 MHz instrument with Auto XDB (direct configuration) probehead was used. For proton-detected measurements: 1H and 15N GHMBC spectra, the 600 MHz instrument with Auto XID (indirect configuration) was applied. For both acquisition and spectrum processing, standard Agilent software (Vnmrj 3.1, Agilent Technologies, Inc., Santa Clara, CA, United States) was used.

Thermogravimetric results (TG) were obtained with the use of the Mettler-Toledo 851e instrument (Mettler-Toledo (Schweiz) GmbH, Greifensee, Switzerland). Measurements were carried out in the argon atmosphere (flow rate 60 mL∙min−1) in the temperature range of 25–900 °C, with a constant heating rate equal to 10 °C∙min−1. Temperature was measured by Pt-Pt/Rh thermocouple, with an accuracy of ±0.5 °C. A sample with a mass of 6.3400 mg was put into the 150 μL open corundum vessel. The gaseous products of the decomposition were analyzed by a quadrupole Balzers ThermoStar mass spectrometer apparatus.

Differential scanning calorimetry (DSC) analysis was performed using a Mettler-Toledo 821e calorimeter. Measurements were performed with the same heating rate as in the case of TG but in the narrower temperature region of 25–600 °C. The examined sample (mass = 18.5730 mg) was enclosed in a partially open (with micro hole) aluminum vessel (40 μg).

The Raman spectrum was recorded on the MultiRAM FT Raman Bruker spectrometer (Bruker Optik GmbH, Ettlingen, Germany), with a 1064 nm laser (150 mW power) as an excitation source. The resolution was set at 2 cm−1. A total of 128 scans (50–3500 cm−1 wavenumber) were collected.

The IR vibrational spectra were measured using a Fourier transform Bruker Vertex 70v vacuum spectrometer (Bruker Optik GmbH, Ettlingen, Germany). Two spectral ranges were explored: middle infrared (MIR) and far infrared (FIR). The data were collected in the 4000–400 and 600–30 cm−1 wavenumber regions, respectively, with a resolution equal to 2 cm−1. Resulting absorption spectra were obtained in a transmission mode by measuring and averaging 32 scans. In the case of MIR measurement, the sample was suspended in a Nujol mull and placed between two KBr discs. The sample prepared in this way was protected against the influence of environmental effects such as vacuum or humidity. Moreover, one can expect that some molecular groups (for example, methyl groups) presented in the studied system may reorientate. This motion can be strongly affected or even blocked when the sample is prepared in the form of a pressed pellet under high pressure (10 tons). In the case of FIR measurements, the sample was prepared in the following manner. About a few milligrams of sample was mixed and grinded with Apiezon N greasy. Next, this mixture was put on a polyethylene (PE) disc. Both Apiezon and PE are transparent to radiation within 600–30 cm−1 and do not show significant absorption peaks.

Additionally, one spectrum at room temperature was collected as sample in a KBr pellet form. This was done to check the spectral range covered by the Nujol bands. As stated above, pressing the sample may cause some molecular reorientations to be blocked by matrix effects.

Temperature-dependent IR measurements were performed using an Advanced Research System DE-202A cryostat (Advanced Research System, Inc., Macungie, PA, USA). This device was coupled to an ARS-2HW helium compressor, which works in a closed cycle manner. A diode temperature sensor was mounted near the sample. Temperature was controlled with the LakeShore 331S device, with an accuracy equal to 0.1 K. The sample was mounted at room temperature and IR data were registered upon cooling with a step 10 K. After the desired temperature was reached, the system was left for approximately 3 min to stabilize and after this time, the spectrum was collected. KRS-5 and PE windows were used in the cryostat for MIR and FIR data collection, respectively.

The incoherent inelastic/quasielastic neutron scattering spectra were measured using the time-of-flight method on a NERA spectrometer [26] at the high-flux pulsed reactor IBR-2 in Dubna (Russia) at a temperature of 10 K. The spectra were converted from neutrons per channel to S(Q, ω) scattering function per energy transfer. At the energy transfer between 5 and 1200 cm−1, the relative INS resolution was estimated to be ca. 3%. The S(Q, ω) function (scattering law) for INS, as described in [27,28,29], can be expressed in the form of an isotropic harmonic oscillator:

SQ,nωiQUi2nn!·exp(QUTot2), (1)

where the ωi and Ui denote the frequency and the mean square displacement of the atoms in the i-th mode, respectively. Q stands for the momentum transfer at which the data were gathered. UTot describes the total root square displacement of all atoms in all modes. Fundamental modes are for n = 1, while n ≥ 2 is responsible for overtones. This equation is valid for a hydrogenous material measured at low temperatures, and for a spectrometer working with a neutron energy loss.

Vibrational spectra (IR, RS, INS) were computed for the crystalline solid state within the frame of the DFT method [30,31], using a CASTEP software [32]. As a first step in the calculation, the experimental geometry (taken from our X-ray single crystal diffraction measurements at 293 K) was optimized. Lattice parameters were held constant and only atomic positions were relaxed. The BFGS minimizer was applied [33]. Next, using the density functional perturbation theory, the eigenvalues and eigenmodes were computed [34]. The PBE (Perdew–Burke–Ernzerhof) generalized gradient approximation (GGA) function was employed in computations [35]. To account for van der Waals interactions, the dispersion correction methodology introduced by Tkatchenko–Scheffler [36] was used. The calculations were done at Γ point using on-the-the-fly generated norm-conserving pseudopotentials. Electronic states were sampled on a 2 × 1 × 1 Monkhorst–Pack grid [37] with the plane-wave cut-off energy equal to 830 eV. The convergence threshold for SCF iterations was set at 5 × 10−10 eV/atom. The optimization parameters were set as follows: total energy convergence tolerance, 5 × 10−6 eV/atom; maximum force tolerance, 0.01 eV/Å; and maximum displacement tolerance, 5 × 10−4 Å [38]. The INS spectrum was simulated based on the results obtained from DFT calculations. Equation (1) allows one to predict INS features if the eigenvectors and eigenvalues are known, for example, from DFT calculations. The AbINS [39] plugin for MANTID [40] program was used for this purpose.

3. Results and Discussion

3.1. X-ray Diffraction Studies

Crystal data, data collection, and structure refinement details for the co-crystal of N,N-dimethylglycine–chloranilic acid at 293 K are shown in Table 1. The crystal structure has been deposited at the Cambridge Crystallographic Data Centre (deposition number CCDC 2069957). This data can be obtained free of charge via http://www.ccdc.cam.ac.uk/conts/retrieving.html.

Table 1.

Crystal data and structure refinement for N,N-dimethylglycine–chloranilic acid co-crystal.

Empirical Formula C10H11Cl2NO6
Formula weight 312.10
Temperature 293(2) K
Wavelength 1.54184 Å
Crystal system Triclinic
Space group P-1
Unit cell dimensions a = 9.6199(4) Å, α = 97.749(4)°.
b = 12.0092(5) Å, β = 110.173(4)°.
c = 12.8763(6) Å, γ = 111.415(4)°.
Volume 1240.68(10) Å3
Z 4
Density (calculated) 1.671 Mg/m3
Absorption coefficient 4.960 mm−1
F(000) 640
Crystal size 0.2 × 0.2 × 0.03 mm3
Theta range for data collection 5.16 to 71.16°.
Index ranges −11 ≤ h ≤ 10, −14 ≤ k ≤ 14, −15 ≤ l ≤ 15
Reflections collected 15,069
Independent reflections 4409 (R(int) = 0.1036)
Completeness to theta = 71.16° 91.9%
Refinement method Full-matrix least-squares on F2
Data/restraints/parameters 4409/0/361
Goodness-of-fit on F2 0.980
Final R indices (I > 2σ(I)) R1 = 0.0471, wR2 = 0.1233
R indices (all data) R1 = 0.0553, wR2 = 0.1283
Largest diff. peak and hole 0.546 and −0.303 e·Å−3

N,N-dimethylglycine–chloranilic acid co-crystal crystallizes in the centrosymmetric triclinic P-1 space group. The independent part of the unit cell is composed of four ions: two N,N-dimethyl glycine cations (denoted as A and B) and two chloranilic acid anions (denoted as C and D, respectively). The numbering scheme of the molecules is depicted in Figure 1, which shows only one of the cation–anion pairs.

Figure 1.

Figure 1

Numbering scheme for N,N-dimethylglycine cation (bottom) and chloranilic acid anion (top). The independent amino acid molecules are denoted with the capital letters A and B, whereas the chloranilic acid are indicated with C and D, respectively. Independent molecules of every kind have very similar geometries.

Cations and anions form a layered structure where the alternating anion and cation layers are perpendicular to the 0, −1, 1 direction. The layers are interlinked with each other in a series of strong N–H···O and O–H···O hydrogen bonds. Interestingly, there are no hydrogen bonds between molecules within a layer (neither chloranilic acid nor amino acid). The crystal packing diagram in the a-direction is shown in Figure 2.

Figure 2.

Figure 2

The crystal packing of N,N-dimethylglycine–chloranilic acid 1:1 shown in the a-direction. The N–H···O and O–H···O hydrogen bonds are shown as dashed lines.

The selected bond lengths as well as bond and torsion angles for the co-crystal observed at room temperature and calculated for the optimized geometry (PBE + vdW level) are presented in Table 2. We can see slight differences between the experimental and optimized values (before and after geometry optimization). Generally, the values calculated and determined experimentally for bond length and angles are close to each other. The biggest differences are observed for X–H bond lengths (X:–C, N, O). It is well known that these distances are underestimated in the case of X-ray measurements. The more reliable distances can be obtained from neutron diffraction measurements. Some of the torsion angles are negative. The values of these angles should be in the range of 0–180 degrees. If the value exceeds 180 degrees, then the angle measured in the opposite direction is given. The sign of this angle is associated with the direction in which the angle is given, i.e., if it is clockwise or anticlockwise.

Table 2.

Selected bond lengths, bond and torsion angles of N,N-dimethylglycine–chloranilic acid (DMG+–CLA) co-crystal at room temperature.

Coordinates Experimental X-ray Calculated CASTEP/PBE + vdW Coordinates Experimental X-ray Calculated CASTEP/PBE + vdW
Bond lengths (Å) N1A–C4A–H4A1 109.5(1) 107.8
C1A–C2A 1.512(3) 1.521 N1A–C4A–H4A3 109.5(1) 109.2
C1C–C2C 1.466(4) 1.452 N1B–C3B–H3B2 109.5(1) 108.6
C1D–C2D 1.458(4) 1.449 O1A–C1A–O2A 121.8(2) 121.8
C2A–H2A1 0.971 1.097 O1B–C1B–C2B 122.2(2) 122.6
C2A–H2A2 0.970 1.097 O1D–C1D–C6D 118.8(2) 118.2
C2C–C3C 1.332(4) 1.363 O2A–C1A–C2A 116.1(2) 115.9
C3A–H3A1 0.960 1.091 O3D–C3D–C2D 122.5(2) 123.0
C3C–C4C 1.512(4) 1.513 O4D–C4D–C3D 115.6(2) 115.0
C4A–H4A1 0.960 1.092 O6D–C6D–C5D 126.6(2) 126.1
C4C–C5C 1.421(4) 1.422 Torsion angles (°)
C5C–C6C 1.371(4) 1.395 H2A–O2A–C1A–O1A −180(3) 179.1
Cl2C–C2C 1.723(3) 1.731 H2A–O2A–C1A–C2A 1(3) −0.2
Cl5D–C5D 1.731(3) 1.737 H1A–N1A–C2A–H2A2 76 73
N1A–H1A 0.860(4) 1.061 H1A–N1A–C3A–H3A1 −62 −57
N1A–C3A 1.490(4) 1.505 H1A–N1A–C3A–H3A2 178 −176.1
N1A–C4A 1.484(3) 1.504 H1A–N1A–C3A–H3A3 58 63.7
N1A–C2A 1.483(2) 1.498 C2A–N1A–C4A–H4A1 −179.7 171.9
O1A–C1A 1.208(4) 1.225 C2A–N1A–C4A–H4A2 60.3 52.5
O2A–H2A 0.820(4) 1.032 C3A–N1A–C4A–H4A2 −174.7 177.4
O2A–C1A 1.303(2) 1.320 C3A–N1A–C4A–H4A3 65.4 57.6
O2B–H2B 0.730(4) 1.030 O2A–C1A–C2A–N1A −173.9(2) −172.6
O3D–C3D 1.325(3) 1.319 O2A–C1A–C2A–H2A1 52.8 −50.8
O4C–C4C 1.227(3) 1.243 H2B–O2B–C1B–C2B −3(3) −0.8
O3D–H3D 0.840(4) 1.002 C3B–N1B–C2B–C1B −162.1(2) −161.2
Bond angles (°) C3B–N1B–C2B–H2B1 76.7 75.5
C1A–C2A–H2A1 109.2(1) 111.1 C4B–N1B–C2B–H2B1 179.6(2) 177.7
C1B–C2B–H2B2 109.1(1) 109.0 C2B–N1B–C3B–H3B1 180 173.4
C1C–C2C–C3C 120.7(2) 120.2 C2B–N1B–C3B–H3B2 59.8 53.9
C1C–C6C–C5C 117.8(2) 118.4 H1B–N1B–C4B–H4B2 179 −175.3
C2A–N1A–C3A 112.4(2) 112.2 C2B–N1B–C4B–H4B3 −59.8 −55.6
C2A–N1A–C4A 111.0(2) 111.1 O1B–C1B–C2B–N1B 4.3(4) 4.3
C2C–C1C–C6C 118.2(2) 118.3 O2B–C1B–C2B–N1B −176.2(2) −176.4
C2B–N1B–C4B 110.4(2) 110.1 H3C–O3C–C3C–C4C 0(3) −3.7
C3C–C4C–C5C 117.2(2) 117.8 C6C–C1C–C2C–Cl2C 176.7(2) 177.1
C4C–C5C–C6C 123.7(2) 122.4 O1C–C1C–C6C–C5C −176.2(2) −175.5
Cl2C–C2C–C1C 117.5(2) 118.6 C1C–C2C–C3C–O3C −179.0(2) −178.6
Cl2C–C2C–C3C 121.7(2) 121.0 O3C–C3C–C4C–O4C 4.1(3) 4.2
H1A–N1A–C2A 107.0(3) 108.0 O4C–C4C–C5C–Cl5C −4.7(4) −5.3
H1A–N1A–C4A 108.0(3) 106.2 C3C–C4C–C5C–C6C −6.1(4) −5.7
H2A–O2A–C1A 110.0(3) 114.5 O1D–C1D–C2D–Cl2D 0.7(4) 0.2
H2B–O2B–C1B 118.0(3) 113.9 O1D–C1D–C2D–C3D −178.3(3) −178.3
H2B1–C2B–H2B2 107.8(1) 107.2 O1D–C1D–C6D–C5D −179.9(2) −179.5
H3A1–C3A–H3A2 109.4(1) 109.4 Cl2D–C2D–C3D–C4D −179.7(2) −179.9
H3C–O3C–C3C 107.0(3) 108.0 C1D–C2D–C3D–C4D −0.7(4) −1.6
H4A1–C4A–H4A3 109.5(1) 111.0 C2D–C3D–C4D–C5D −2.0(4) −0.5
H4B2–C4B–H4B3 109.5(1) 110.1 C3D–C4D–C5D–C6D 4.0(4) 2.9
N1A–C2A–H2A1 109.2(1) 108.8 C4D–C5D–C6D–O6D 176.4(2) 176.9
N1A–C3A–H3A1 109.5(1) 108.8 C4D–C5D–C6D–C1D −3.0(4) −3.0

Table 3 lists the respective hydrogen bond geometry. The N–H amino groups are involved in bifurcated hydrogen bonds, with two neighboring carbonyl oxygen atoms of the chloranilic acid molecule. There are three intermolecular hydrogen bonds for every type of independent molecule: A, B, C, and D. In C and D molecules, there are intramolecular hydrogen bonds: O3D–H3D···O4D and O3–H3C···O4C. However, the same donor in either the C or D molecule also takes part in the intermolecular bonds with molecule A or B, respectively. In this case, the rings D···A···D’···A’ and C···B···C’···B’, or the chains ··D···A···C···B·· are formed; thus, the graphic is described as “chains of rings” (see Figure 3). The structure is also interesting and much more complicated because there are two different intermolecular binary hydrogen bonds with two donors and one acceptor: between molecules A, B (donors) and C or D (acceptor). Therefore, there are also other hydrogen bond chains such as: ··C···B···D···A·· along the crystallographic a-direction, where donors belong to the A and B molecules, whereas acceptors are in C and D, respectively.

Table 3.

Hydrogen bonds for the N,N-dimethylglycine–chloranilic acid co-crystal (Å and °).

D–H···A d(D–H) d(H···A) d(D···A)
O(2A)–H(2A)···O(6C) 0.82(3) 1.76(3) 2.555(2)
N(1A)–H(1A)···O(1D)#1 0.86(3) 2.39(3) 2.933(2)
N(1A)-H(1A)···O(6D)#1 0.86(3) 1.99(3) 2.813(2)
O(2B)-H(2B)···O(6D)#2 0.73(3) 1.85(4) 2.578(2)
N(1B)-H(1B)···O(1C) 0.83(3) 2.43(3) 2.971(2)
N(1B)-H(1B)···O(6C) 0.83(3) 2.03(3) 2.826(2)
O(3C)-H(3C)···O(1B)#3 0.87(4) 2.11(4) 2.810(2)
O(3D)-H(3D)···O(1A)#4 0.84(4) 2.15(4) 2.765(2)

Symmetry transformations used to generate equivalent atoms: #1, −x + 1, −y + 1, −z + 2, #2, −x, −y + 1,−z + 2, #3, −x, −y + 1,−z + 1, #4, x, y + 1, z.

Figure 3.

Figure 3

Characteristic ring aggregates in N,N-dimethylglycine–chloranilic acid 1:1.

Figure 4a depicts a Hirshfeld surface. It was generated using a CrystalExplorer program [41] for room temperature crystal structures. The Hirshfeld surface is an interesting concept, allowing one to visualize the most important interactions that can be found in the studied sample. Many properties can be mapped to such surfaces. We have plotted the so-called dnorm parameter. It provides information about intermolecular contacts between atoms. If the contacts are closer than the sum of the van der Waals radii of the atoms, the appropriate region of the surface is red-colored. These regions correspond to hydrogen bonds. Blue regions visible on the surface indicate that there is no close contact between atoms inside and outside of the surface. A similar analysis was performed, for example, for the 2,6-dimethylpyrazine and picric acid co-crystal [42]. The other properties that can be mapped onto the surface are the di and de parameters. The di and de parameters describe the distance from the surface to the nearest atom inside or outside the surface, respectively. In Figure 4b, the fingerprint plots (de versus di) for the investigated co-crystal are shown. In this picture, contributions from the main interactions were marked together with the percentage of the total area. The nine presented interactions contribute 99.3% of the total area. The C–Cl interactions contribute ~0.8%. This is the smallest contribution and was omitted in the figure. As can be seen in this figure, the O–H interactions contribute 41.8% (Figure 4b). This is the highest contribution presented in the fingerprint plot.

Figure 4.

Figure 4

Figure 4

(a) Hirshfeld surface analysis for the N,N-dimethylglycine–chloranilic acid co-crystal. Parameters mapped onto the surface dnorm. (b) Fingerprint plots generated with relative percentage contributions of particular intermolecular contacts to the Hirshfeld surface area.

Two characteristic sharp spikes are clearly visible. The second highest area is covered by H–Cl interactions (22.6%). The remaining intermolecular interactions contribute less than 10% of the total surface. During the analysis, it was found that particular interactions contribute to the fingerprint in the following amounts: O–H (41.8%), H–Cl (22.6%), H–H (7.3%), Cl–Cl (7.1%), O–C (6.4%), C–C (6.2%), H–C (4%), O–O (2.9%), O–Cl (1%), C–Cl (0.8%). The other interactions (O–N, H–N, N–N, N–C, N–Cl) are not visible. It can be concluded that hydrogen bonds are the most important interaction found in the studied co-crystal.

3.2. NMR Spectroscopy

The structure of the complexation product of chloranilic acid (CLA) and N,N-dimethylglycine (DMG) was investigated in both a DMSO solution and in the solid state. The results of the NMR measurements of substrates and the complexation product are shown in Table 4.

Table 4.

NMR data for the N,N-dimethylglycine–chloranilic acid co-crystal (ppm).

Reagents Used and Measurement Conditions C=O C–OH C–O C–Cl CH3 CH2 COOH N
CLA in DMSO 165.9 110.01
DMG in DMSO 43.8
2.59
60.8
3.24
167.2 −345.2
CLA solid state CPMAS 176.0 # 154.9 # 110.8 114.3
DMG solid state CPMAS 41.9, 43.3, 44.1, 44.5 58.7,
59.3
169.4, 170.97 −342.4
CLA + DMG Complex
DMSO
167.9
ν 1/2 = 88 Hz
105.1 43.75
2.80
56.97
4.04
168.0
ν 1/2 = 1 Hz
−344.1
CLA + DMG Complex
CPMAS
178.7 ”
170.1 *”
154.7 ” 164.5 ” 110.0
106.3
45.7 60.1 170.1 * −342.2
CLA + TMG 1:1 in DMSO 167.95 105.3
CLA + double excess of TMG in DMSO 172.4 105.4
Dimethylglycine
In DMSO
43.8
2.59
60.8
3.24
167.2 −345.2
Dimethylglycine + TMG in DMSO 45.76
2.10
65.25
2.56
173.0 −357.2
N-dimethylglycine
D2O
43.45
2.70
59.82
3.58
170.25 −342.1
N-dimethylglycine in D2O + HClO4 43.73
2.84
57.33
3.93
168.13 −344.8

#—avaraged value 165.45 ppm. ”—averaged value 167 ppm. *—overlapped signals (double intensity). data in bold and underlined: proton chemical shifts. ν 1/2 half height line width.

The first problem is the structures of CLA and DMG in both phases. The carbon spectrum of CLA in the DMSO solution only has two signals, one from chlorine-substituted carbon atoms and the second one from atoms connected with oxygen atoms, where two protons in the molecule are distributed over four positions. A completely different situation was found in the CPMAS carbon spectrum of CLA. In this case, four well-separated signals were detected. In the region typical for oxygen bonded to carbon atoms, we have two signals: 176.0 ppm typical for the carbonyl group and 154.9 ppm characteristic of phenol carbon atoms. This indicates that in the solid state the proton exchange process is stopped, probably by intermolecular hydrogen bonds. Two signals from chlorine-substituted carbon atoms suggested that two hydroxyl groups are located in the 1,3 position leading to an unsymmetrical structure, in contrast to the 1,4 symmetrical structure. A similar relation between spectra in the solution and the solid states is observed for the complexation product. Once again, a dynamic averaged structure leading to two signals was found in DMSO. The most interesting finding is the low-field signal δ = 167.9 ppm assigned to the oxygen-substituted sites. This signal is low-field shifted as compared to the CLA spectrum, which suggests that a proton-transfer process from CLA to DMG occurs. Additionally, this signal is very broad (ν 1/2 = 88 Hz), which shows that the exchange process is relatively slow, close to the coalescence region. In the solid-state spectrum of the complex, four carbon signals in the low-field region were found. The position of those signals suggests that one proton was transferred from CLA to DMG, forming a nonsymmetrical structure with two nonequivalent C=O groups and one C–OH moiety. The averaged chemical shift difference between neutral C=O and deprotonated C–O sites is close to 10 ppm. This is close to the effect observed in appropriate chemical shifts between CLA and the complex with DMG in the DMSO solution. In reality, this effect is equal to 2 ppm, but we have to remember that it is averaged over four positions. To confirm that consideration, the deprotonation reactions of CLA by tetramethylguanidine (TMG) were performed. The addition of one equivalent of TMG to CLA in the DMSO solution moves the averaged oxygen-bonded carbon atom low-field signal to a value of 167.95 ppm (very close to the position of this signal in the complex with DMG), the next equivalent of TMG moves this signal to 172.4 ppm, indicating further deprotonation reaction. The formation of a 1:1 complex of CLA and DMG in a DMSO solution was confirmed by diffusion measurement. The molecular weight of the expected complex calculated from the diffusion coefficient is Mw = 352. The Mw value calculated from the molecular formula is 209 + 103 = 312. In this measurement, the calculated Mw value of DMSO was 67, while the correct value is 83. The relatively small difference between measured and calculated Mw values confirms the reliability of this method.

In essence, the complexation spectral effect should be visible on the NMR signal positions of the DMG molecule. Unfortunately, the situation is much more complicated. First of all, using the diffusion coefficient measurement with the DOSY (Diffusion-Ordered Spectroscopy) method, we found that in the DMSO solution, DMG exists as a dimer (measured Mw = 196, calculated 103), whereas in D2O it exists as a single molecule (measured Mw = 106). In the dimer structure presented in the DMSO, both proton acceptor positions are partially protonated. In the solid state, the C=O signal from DMG is slightly shifted downfield, indicating some deprotonation process on this position as compared to the DMSO solution. Since the chemical shifts of the C=O atom in DMG in both the pure sample and the potential complex are almost the same, we can state that the proton position in both species is the same, which suggests that the proton transfer responsible for the complex formation occurs in the nitrogen atom. Unfortunately, we are not able to confirm this conclusion on the basis of nitrogen chemical shifts. It is well-known that nitrogen chemical shifts of aliphatic amines are almost insensible in the protonation process. Protonation of such functional groups can produce an effect of only a few ppms in both directions; therefore, this spectral parameter is worthless from this point of view.

3.3. Thermal Analysis (TG, DSC)

To check the stability of the title compound, thermal analysis (TG, DTG, DSC) was performed.

Figure 5 shows the DSC results obtained in the temperature range of 25–600 °C for the N,N-dimethylglycine–chloranilic acid (DMG+–CLA) co-crystal.

Figure 5.

Figure 5

DSC curve for the N,N-dimethylglycine–chloranilic acid co-crystal, registered at a heating rate of 10 °C min−1 in the temperature range of 25–600 °C.

It is worth mentioning that DSC examinations were conducted on a sample that was enclosed in an alumina vessel with a micro hole, whereas the TG analysis was performed on a sample placed in an open pan. The situation of the samples were different therefore the comparison of both results is not straightforward. In the DSC, the released fragment could not be easily removed from the vessel. On the DSC curve one can see a few peaks. The observed features can be explained using a combined approach and taking into account the TG results.

The exothermic peak, presented on the DSC curve at 197 °C, relates to the decomposition process. The sample decomposes without melting. This situation is different from that which was observed for the L leucine–chloranilic acid complex previously studied [14]. The second peak (at 271.0 °C), followed by another (at 320 °C) not-well-resolved and large exothermic peak are most probably connected with the combustion process of the previously released fragments containing carbon, hydrogen, and oxygen. This is a continuous process. Finally, at 546 °C, there is one more anomaly visible, which is smaller than the previous ones.

The DSC measurements were also conducted in the temperature range from 25 °C to −163 °C (on cooling and heating, with a scan rate equal to 10 °C/min). This examination did not reveal any anomaly on the DSC curve. Therefore, there is no phase transition in the solid state within the mentioned temperature region.

Figure 6 shows TG, DTG, and QMS curves recorded for the N,N-dimethylglycine-chloranilic acid complex. The QMS spectra for m/e = 16, 17, 18, 28, and 44 are shown. These lines correspond to the following fragmentation moieties: NH2/O, NH3/OH, H2O, CO, and CO2, respectively. The greatest change in mass occurs at ca. 200 °C. This corresponds very well to the first DSC peak. The fragments described by QMS signals are released and removed from the sample. After further heating, the organic residues are gradually removed. From the results presented above, one can conclude that the decomposition process proceeds in two steps. These results are compatible with the DSC results. In the first step (temperature range 25–277 °C), the complex loses 55.0% of the starting weight. Above 277 °C, further degradation of the sample occurs and a second step can be distinguished. The weight lost was 22.0% and the final mass after the TG experiment (at 900 °C) was 23.0% of the initial mass. The calculated value of the carbon residue (C6) is 23.1%.

Figure 6.

Figure 6

TG, DTG, and QMS curves for the N,N-dimethylglycine–chloranilic acid complex registered at a heating rate of 10 °C min−1 in the temperature range of 25–900 °C.

The thermal decomposition of the investigated complex proceeds through more complicated processes such as decarboxylation, deamination, and condensation reactions. The differential scanning calorimetry (DSC) results show that the decomposition process is more complex than in the case of TG measurements. These differences can be attributed to different measuring conditions. Thermal analysis confirms that the DMG+–CLA co-crystal is stable up to a temperature of 190 °C.

3.4. Vibrational Spectra

The investigated N,N-dimethylglycine–chloranilic acid (DMG+–CLA) co-crystal contains 120 atoms in a unit cell. Hence, one should expect 360 normal modes. 312 of them describe internal normal vibration modes, 21 describe translation, 24 are rotation modes, and 3 are nonactive acoustic modes. The crystal is centrosymmetric, so the mutual exclusion rule applies. Vibrations active in IR spectroscopy are silent in RS and vice versa. According to the selection rules in the Ci factor group, one gets the following irreducible representation: Γvib = 180Ag + 180Au (including acoustic modes). Modes with Au symmetry are active only in IR, while Ag modes are active only in Raman spectra for k = 0. Details are described in Table 5.

Table 5.

Classification of the fundamental modes (k = 0) for the N,N-dimethylglycine –chloranilic acid (DMG+–CLA) co-crystal.

UCG Lattice Modes Internal Modes Selection Rules
Ci Ac. Trans. Rot. DMG+ CLA IR Raman
Ag 12 12 90 66 x2, y2, z2,
xy, xz, yz
Au 3 9 12 90 66 x, y, z

UCG—unit cell (factor) group; Ac.—acoustic modes; Trans.—translation modes; Rot.—rotation modes.

IR, Raman, and INS spectra measured at 290 K, 290 K, and 10 K, respectively, as well as their calculated in periodic boundary condition counterparts (range 800–50 cm−1) are presented in Figure 7.

Figure 7.

Figure 7

Comparison of experimental and calculated (at PBE + vdW level): (a) IR, (b) RS, and (c) INS spectra for the N,N-dimethylglycine–chloranilic acid complex in the region of 800–50 cm−1.

The most intensive peaks presented in the INS spectrum are connected to torsion vibrations of the methyl CH3 groups. These bands are located between 200 and 285 cm−1 at the following wavenumbers: 211, 223, 235, and 284 cm−1. The calculated values are shifted slightly with respect to the experimental ones and are found at 209, 229, 265, and 276 cm−1. Very good agreement between experiment and calculations is evident. The IR and RS spectra in the energy range of 3500–450 cm−1 are shown in Figure 8.

Figure 8.

Figure 8

Comparison of experimental and calculated (at PBE + vdW level): (a) IR and (b) RS spectra for the N,N-dimethylglycine–chloranilic acid complex in the region of 3500–450 cm−1.

Table 6 lists the experimental IR, Raman and INS wavenumbers along with the calculated values. The assignment, which was accomplished based on available literature data [18,19,23,43] and visualization of modes in Jmol software [44], is also provided.

Table 6.

Observed and calculated frequencies for the N,N-dimethylglycine–chloranilic acid (DMG+–CLA) co-crystal (cm−1). The structure is centrosymmetric; thus, vibrations active in IR are not active in Raman and vice versa.

Experimental Calculated Approximate Assignments
IR (290 K) Raman (290 K) INS CASTEP/PBE + vdW
3122(IR) 3126(R) CH3 str. asym. (DMG+)
3120(IR) 3120(R) CH3 str. asym. (DMG+)
3119(IR) 3119(R) CH3 str. asym. (DMG+), O–H str. (CLA)
3118(IR) 3118(R) CH3 str. asym. (DMG+)
3179 3113(IR) 3112(R) O–H str. (CLA)
3102(IR) 3102(R) CH3 str. asym. (DMG+)
3050 3051 3052(IR) 3052(R) CH2 str. asym. (DMG+)
3032 3044(IR) 3044(R) CH2 str. asym. (DMG+)
3018(IR) 3017(R) CH3 str. sym. (DMG+)
3013(IR) 3013(R) CH3 str. sym., CH2 str. asym. (DMG+)
3006(IR) 3006(R) CH3 str. sym. (DMG+)
3006 2996 3000(IR) 3000(R) CH2 str. sym. (DMG+)
2974 2947 2976 2995(IR) 2995(R) CH2 str. sym. (DMG+)
2911 2887 2857(IR) 2855(R) N–H str. (DMG+)
2863 2840 2874 2846(IR) 2846(R) N–H str. (DMG+)
2788 N–H str. (DMG+)
2722 2698 2720 2595(IR) 2592(R) O–H str. (DMG+)
2654 2626 2562(IR) 2558(R) O–H str. (DMG+)
2510 2468 O–H str. (DMG+)
2441 O–H str. (DMG+)
1994 C–H2 bend. overtone
1874 CH2 bend. + CH2 wagg. summation
1734 1682(IR) 1684(R) C=O str., NH+ bend., COH bend. (DMG+)
1727 1726 1671(IR) 1670(R) C=O str., NH+ bend., COH bend. (DMG+, CLA)
1629(IR) 1629(R) C=O str., C−C str., COH bend. (CLA)
1627(IR) 1628(R) C=O str., C−C str., COH bend. (CLA)
1676 1679 1606(IR) C=O str., C−C str., COH bend. (CLA), NH+ bend. (DMG+)
1655 1640 1575(IR) 1581(R) C=O str., C−C str., COH bend. (CLA)
1523 1541 1582 1515(IR) 1529(R) NH+ bend. (DMG+), C−C str., COH bend. (CLA, DMG+)
1480 1483 1470(IR) 1470(R) CH3 bend., NH+ bend. (DMG+)
1464 1453(IR) 1453(R) CH3 bend., NH+ bend. (DMG+)
1435(IR) 1435(R) CH3 bend., NH+ bend. (DMG+)
1447 1455 1433(IR) 1433(R) CH3 bend., NH+ bend. (DMG+)
1431(IR) 1432(R) CH3 bend., NH+ bend. (DMG+)
1399(IR) 1399(R) CH3 bend., NH+ bend., CH2 bend. (DMG+)
1416 1396(IR) 1396(R) CH3 bend., NH+ bend., CH2 bend. (DMG+)
1407 1403 1371(IR) 1372(R) CH3 bend., NH+ bend., CH2 wagg., COH bend. (DMG+)
1390 1351(IR) 1352(R) CH2 wagg., COH bend. (DMG+)
1391 1346(IR) 1347(R) CH2 wagg., COH bend. (DMG+, CLA),
C−C str. (CLA)
1372 1324(IR) 1324(R) C=O str., C−C str., COH bend. (CLA)
1363 1345 1365 1315(IR) 1318(R) C=O str., C−C str., COH bend. (CLA)
1328 1277(IR) 1276(R) CH2 twist. (DMG+), COH bend., CH3 rock.(CLA)
1279 1294 1262(IR) 1262(R) C−C str., COH bend. (CLA), CH2 wagg. (DMG+)
1254(IR) 1251(R) C−C str., C−O str. (CLA)
1248(IR) 1247(R) C−C str., C−O str. (CLA), CH2 wagg., C−O str. (DMG+)
1255 1259 1243(IR) 1241(R) C−C str., COH bend. (CLA), CH2 rock., CH3 rock. (DMG+)
1214(IR) 1215(R) CH3 rock. (DMG+)
1160(IR) 1161(R) CH3 rock. (DMG+), COH bend. (CLA)
1177 1175 1154(IR) 1152(R) CH3 rock. (DMG+), COH bend. (CLA)
1140 1142 1118(IR) 1117(R) CH3 rock. (DMG+)
1114 1121 1075(IR) 1071(R) OH bend., CH3 rock. (DMG+)
1062(IR) 1057(R) OH bend., CH3 rock. (DMG+)
1054 1055 1025(IR) 1025(R) NH+ rock., CH3 rock. (DMG+)
1017 1020 990(IR) 989(R) C−N str., C−C str., CH3 rock. (DMG+)
976(IR) 976(R) C−N str., CH3 rock., CH2 rock. (DMG+)
1001 1002 973(IR) 974(R) C−N str., CH3 rock., CH2 rock. (DMG+)
962(IR) 961(R) C−C str., COH bend. (CLA)
980 983 958(IR) 958(R) C−C str., COH bend. (CLA)
957 961 937(IR) 936(R) C−N str., CH3 rock., CH2 rock. (DMG+)
904 886 910 889 891(IR) 888(R) C−C str., C−N str. (DMG+), OH tors. (CLA)
870(IR) 868(R) OH tors. (CLA)
862 863 859(IR) 856(R) OH tors. (CLA)
839 837 844(IR) 844(R) C−N str. (DMG+)
771 790 798 781 817(IR) 816(R) C−C str., C−Cl str. (CLA)
747 752 755 762(IR) 762(R) Ring def. (CLA)
675 677 679 663(IR) 663(R) COOH bend. (DMG+)
616 608 621 567(IR) 561(R) Ring def. (CLA), COOH rock. (DMG+)
571 573 570 555(IR) 556(R) CH2 rock., COOH rock. (DMG+)
535 536 539 530(IR) 530(R) Ring def. (CLA)
504 505 509 504(IR) 505(R) Ring def. (CLA)
461 466 469 486(IR) 487(R) C−C−N bend., C−N−C bend., N−CH3 bend. (DMG+)
434 436 431(IR) 430(R) Ring def. (CLA), C−C−N bend., N−CH3 bend. (DMG+)
405 408 417 401(IR) 400(R) Ring def. (CLA)
361(IR) 361(R) Ring def. (CLA), C−C−N bend. (DMG+)
372 369 352 380 364 358(IR) 356(R) C=O bend., C−O bend. (CLA), CH2 rock. (DMG+)
340(IR) 340(R) C−N−C bend., N−CH3 bend. (DMG+), COH bend. (CLA)
307 300(IR) 300(R) Ring def. (CLA)
286 287 284 281(IR) 280(R) COH bend. (CLA), C−N−C bend., CH3 tors. (DMG+)
266(IR) 266(R) CH3 tors., CH2 rock. (DMG+)
232(IR) 232(R) CH3 tors., CH2 rock. (DMG+)
236 236 235 227(IR) 229(R) CH3 tors. (DMG+)
213(IR) 212(R) CH3 tors. (DMG+)
209(IR) 208(R) CH3 tors. (DMG+)
220 214 223 205(IR) 203(R) Ring tors. C=O wagg. (CLA)
208 212 200(IR) 199(R) Ring tors. C=O wagg. (CLA)
175 178(IR) 178(R) C−C tors. (DMG+)
156 163 163 168(IR) 168(R) C−C tors. (DMG+)
141 152 149(IR) 151(R)
137 135 146 138(IR) 140(R) Ring tors. (CLA), C−N tors. (DMG+)
122 127 120 25–135 Lattice vibrations
101 92 92 94
65 79
54 59 64

Careful inspection of the middle infrared spectrum reveals that the bands are located on a high background. This background is rather bumpy, not flat. There are three distinct and broad Gauss-like curves illustrating the changes in the background. These peaks are centered at approximately 1300, 1895, and 2722 cm−1. Such a picture reassembles the so-called Hadżi trio and is characteristic of systems with strong N+–H···O hydrogen bonds. It is worth noting that similar features were also observed for other complexes containing CLA as one of the components [15,43,45,46].

In the IR experimental spectra at 3179 cm−1 (290 K), the O–H stretching vibration modes of CLA molecules are visible. The calculated wavenumber for this vibration is shifted towards the lower values and is equal to 3113 cm−1. In the Raman spectrum, we do not observe the band associated with this vibration. A low intensity shoulder band at 3050 cm−1 (IR spectrum) is connected to the asymmetrical stretching CH3 modes of DMG+. The Raman and theoretical bands are almost in the same place. In the region of 3044–2995 cm−1 of the calculated spectra, asymmetrical and symmetrical stretching CH3 and CH2 modes are observed.

In the 2900–2788 cm−1 N–H region, stretching vibrations of the aliphatic amino acid (DMG+) are visible. The calculated bands are sharp, very intensive, and well-separated; however, in the experimental IR spectra, we can observe a broad band with the shoulder bands. In the IR spectra between 2700 and 2400 cm−1 we can observe hydrogen bond vibrations and the O–H stretching vibration of DMG+. In the RS spectrum, there are no visible bands in this region (see Figure 8). A very broad peak (IR spectrum) with its maximum at 1874 cm−1 is connected to the overtone (CH2 bend.) and summation (CH2 bend. + CH2 wagg.) vibration modes. We do not observe these bands in the calculated spectra. In the IR experimental spectrum, the intensive peak at 1523 cm−1 relates to the bending NH+, COH modes, whereas in the experimental RS spectra this band is of low intensity. Near 1480–1400 cm−1 bending CH3, NH+, CH2 modes are observed (from DMG+ cation). This region (both IR and RS) is very well-represented by computations. Intensive bands between 1255 and 1000 cm−1 were interpreted, among others, as rocking CH3 rock. (DMG+) modes of N,N-dimethylglycine. The calculated bands are sharp and shifted towards lower wavenumbers (see Figure 8). In the wavenumber range of 1000–600 cm−1 in the IR and RS spectra, we can observe the ring deformation, OH torsion, and COH bending of chloranilic acid anion, as well as C–N stretching, C–C stretching, CH2 rocking, and COOH bending of the N,N-dimethylglycine cation modes. In this range, there is a good agreement between experimental and predicted IR and RS spectra.

In the wavenumber range of 600–50 cm−1, N–CH3 bending, CH2 rocking, C–C–N bending, C–N–C bending, and CH3 torsion modes for N,N-dimethylglycine cation are observed. For the chloranilic acid anion in this same region, ring deformation, COH bending, C=O wagging, and ring torsion modes are also observed. Lattice vibrations are active below 130 cm−1.

3.5. Temperature-Dependent IR Spectroscopy

The IR spectra (both FIR and MIR) were measured for the DMG+–CLA complex at various temperatures. The investigated complex does not exhibit any phase transition in the temperature range of 300–100 K. Hence, for most of the registered bands, no spectacular changes were observed. One can notice the narrowing (increasing intensity) of the peaks during the cooling of the sample. This is natural behavior. The position of the peaks does not change as well. However, in a few wavenumber regions, more significant changes can be noticed. Among the most interesting, four ranges can be highlighted. These are the following: 810–760, 1045–990, 2800–1800, and 3200–3120 cm−1. The first mentioned region is covered by C−Cl str. (CLA) vibrations and is displayed in Figure 9.

Figure 9.

Figure 9

(a) IR spectra in the wavenumber range of 810–760 cm−1 during cooling of the N,N-dimethylglycine–chloranilic acid co-crystal; (b) contour plots of IR spectra in the C−Cl str. (CLA) vibration region.

At room temperature one can see two very broad and diffused bands. During cooling, these two maxima move away from each other. The initially hidden bands emerged from the spectrum and four well resolved peaks are clearly visible at the lowest temperature. The shifting and appearing of these bands are nicely visible in the contour plot figure. In the second region, vibrations connected with C−N str., CH3 rock., CH2 rock. (DMG+) are visible. Here we have a very similar picture. At room temperature two bands can be detected, but at 13 K, five spectral features are present. These changes are depicted in Figure 10. The bands visible in the third wavenumber range (Figure 11) are located on a relatively high background. In this region, the bands are related to overtone (C–H2 bend.) and summation (CH bend. + CH wagg.) vibrations modes and O−H stretching modes (DMG+). Very interesting changes are visible in the fourth mentioned region (Figure 12).

Figure 10.

Figure 10

(a) IR spectra in the wavenumber range of 1045–990 cm−1 during cooling of the N,N-dimethylglycine–chloranilic acid co-crystal; (b) contour plots of IR spectra in the C−N str., CH3 rock., CH2 rock. (DMG+) vibration regions.

Figure 11.

Figure 11

(a) IR spectra in the wavenumber range of 2800–1800 cm−1 during cooling of the N,N-dimethylglycine–chloranilic acid co-crystal; (b) contour plots of IR spectra in the overtone (C–H2 bend.) and summation (CH bend. + CH wagg.) regions, and O–H stretching modes (DMG+).

Figure 12.

Figure 12

(a) IR spectra in the wavenumber range of 3200–3120 cm−1 during cooling of the N,N-dimethylglycine–chloranilic acid co-crystal; (b) contour plots of IR spectra in the H-bonding region (O–H vibration from CLA acid).

In this range, the O–H vibration of CLA acid appears. These atoms are engaged in the formation of hydrogen bonds: O3C–H3C···O1B, O3D–H3D···O1A. Therefore, this vibration is a sensitive indicator of the strength of the formed hydrogen bonds. At high temperatures, the O–H stretching vibration is very broad and blurred. During cooling, one can observe not only a narrowing of this peak but also a significant (18 cm−1) shift towards lower wavenumbers. The center of this peak moves from 3179 cm−1 at 290 K to 3161 cm−1 at 13 K. Such a meaningful shift is, without doubt, connected to an increase in the strength of the hydrogen bonds. The distance between H and O in O–H(CLA)···O(DMG+) (~2.1 Å at room temperature) is reduced (see Figure 1 and Figure 3). The hydrogen pattern in this complex is complicated. Besides the formerly described interactions, there are hydrogen bonds formed between: N1B–H1B(DMG+)···O6C(CLA) (H1B···O6C distance = 2.032 Å, angle N–H–O = 160.9°), N1B–H1B(DMG+)···O1C(CLA) (H1B···O1C distance = 2.436 Å, angle N–H–O = 123.3°), N1A–H1A(DMG+)···O6D(CLA) (H1A···O6D distance = 1.994 Å, angle N–H–O = 158.3°) and N1A–H1A(DMG+)···O1D(CLA) (H1A···O1D distance = 2.393 Å, angle N–H–O = 121.2°). As one can see here, we have two rather short hydrogen bonds (~2 Å) and two slightly longer ones (~2.4 Å). However, the dynamical properties of the N–H···O hydrogen bonds are different in comparison with O–H···O interactions. The bands connected with N–H vibrations do not change their position in IR spectra. This interaction is strong at 293 K and the decrease in temperature does not have a strong impact on the N–H vibration.

Figure 13 shows the selected FIR spectra of the DMG+–CLA co-crystal, within the wavenumber range of 450–80 cm−1, registered during the cooling of the sample from 290 K to 10 K. One band (at 434 cm−1) was chosen for further analysis.

Figure 13.

Figure 13

Selected FIR spectra in the wavenumber range of 450–80 cm−1 during cooling of the N,N-dimethylglycine–chloranilic acid co-crystal from 290 K to 10 K, with the step 10 K.

This peak relates to the N–CH3 bending vibration, and its width decreases during cooling. We performed a band-shape analysis for this band. For such an analysis to be possible, three conditions must be met. The chosen peak should not overlap with others. This suggests that it should be isolated in a sufficiently wide range of wavenumbers. The second condition states that in the analyzed vibration, only one motion of a particular group should be involved. The third and final requirement is that the intensity of the selected peak should not be too low. Looking at Figure 13, one can see that all these conditions were fulfilled: the peak is isolated (both in terms of energy and mode involved) and its intensity is considerable.

During routine data analysis, the stress is usually placed on two spectral features. One can be interested in where the particular band is located on a wavenumber scale and what its intensity is. However, some interesting information are likewise encoded in the width of the peak. The reorientational motion of molecular groups is reflected in the peak width. On cooling, the width of the band decreases. Without going into details, one can say that two relaxation mechanisms may be distinguished: the vibrational and the reorientational relaxation. The band shape analysis is a tool used to extract information regarding the molecular reorientational motion. It is known that the width of the peak (expressed in cm−1) is inversely proportional to the reorientational correlation of time τc. Optical spectroscopies such as IR and RS give access to motions, which occur within a 10−13–10−11 s time scale. This is the so-called time window and it is characteristic of a particular method. The methodology is as follows: One needs to collect vibrational spectra from a wide temperature range. The chosen band is approximated by a bell-shaped curve (Lorentz or Gaussian function), which is fitted to the experimental data in the analyzed region. Next, the temperature dependency of full-width-at-half-maximum (FWHM) is investigated. The theoretical considerations for the order–disorder transition mechanism are given in Ref [47,48].

One of the crucial parameters is the reorientational correlation time τc. It can be described by Equation (2):

τc=τ·expEaRT, (2)

where the molecule residues for a τc time in one potential well and jumps (infinitely fast) to another potential well. The preexponential factor τ denotes the relaxation time at an infinite temperature. Jumps occur through the potential energy barrier Ea. R is the gas constant.

Temperature dependence of the FWHM can be expressed by Equation (3) [47,48,49]:

FWHMT = a+b·T + c·expEaRT, (3)

where the parameters a, b, c, and Ea are obtained during the least square fitting procedure. This formula is fitted to the experimental data. In Equation (3), two parts are clearly visible. The linear part relates to the above-mentioned vibrational relaxation process. It does not strongly depend on temperature. The exponential term describes the reorientational motion.

In the studied system, methyl groups belonging to N,N-dimethylglycine may reorientate and can be responsible for the narrowing of some bands on cooling.

Analysis of the temperature dependency of the full-width-at-half-maximum (FWHM) was carried out for the band at 434 cm−1, associated with the N–CH3 bending mode.

Figure 14 shows the changes of peak width while the temperature decreases. Inspection of this figure reveals that FWHM decreases when the sample is cooled. In the temperature region of 295–140 K, this change is well-described by an exponential function. Further cooling of the sample has a much smaller impact on FWHM. The dependency below 140 K is well-described by a linear function. The function reaches a plateau. It indicates that considering the time window, the motion of the N–CH3 group is slowed down. Equation (3) was fitted to the experimental data in the temperature region of 290–10 K. Parameters obtained during this procedure are gathered in Table 7. As a result of the performed analysis, the mean value of the activation energy Ea was estimated. Ea is equal to 6.7 kJ mol‒1. Comparing this value with other results obtained for compounds containing methyl groups, one can see that they fall in the same range. Based on the band shape analysis performed for the N,N-dimethylglycine–chloranilic acid co-crystal, it can be said that the fast N–CH3 group reorientation slows down below 140 K and the correlation time τc is greater than 10−11 s.

Figure 14.

Figure 14

Temperature evolutions of FWHM of the band at 434 cm−1 associated with the N–CH3 bending mode. The solid line represents fitting of Equation (3) to the experimental data.

Table 7.

The fitted parameters: a, b, c, and Ea for the temperature dependence of FWHM of the infrared band at 434 cm−1, associated with the N–CH3 bending mode obtained in the temperature range of 290–10 K for the N,N-dimethylglycine–chloranilic acid (DMG+–CLA) co-crystal (cm−1).

Parameter Value
a (cm−1) 6.53 ± 0.07
b (cm−1·K) 2.65 × 10−3 ± 8.6 × 10−5
c (cm−1) 44.00 ± 9.18
Ea (kJ·mol−1) 6.7 ± 0.6

4. Conclusions

At room temperature, the new co-crystal N,N-dimethylglycine–chloranilic acid crystallizes in a triclinic crystal system within space group No.2 = P-1, with four molecules in the unit cell. The following unit cell constants were determined: a = 9.6199(4) Å, b = 12.0092(5) Å, c = 12.8763(6) Å, α = 97.749(4)°, β = 110.173(4)°, and γ = 111.415(4)°.

From NMR and X-ray measurements, it was found that N,N-dimethylglycine exists in the cation form (protonated form) and chloranilic acid exists in the anion form (deprotonated form).

Cations and anions form a layered structure. They are bonded to each other by a series of intermolecular N+–H···O and O+–H···O hydrogen bonds. The N–H amino groups are involved in bifurcated hydrogen bonds, with two carbonyl oxygen atoms of neighboring chloranilic acid molecules.

X-ray diffraction and IR investigation confirm the presence of a strong N+–H···O hydrogen bond in the studied co-crystal. In the IR spectrum, one can observe a broad, continuous absorption with a well-separated and so-called Hadżi trio, typical of strong hydrogen bonds with peaks at approximately 1300, 1895, and 2722 cm−1.

Thermal analysis showed that the investigated complex is stable up to 190 °C. It subsequently decomposes, and combustion processes are observed. The normal modes were described and analyzed as a function of temperature. During the cooling of the complex from room temperature to 13 K, we observe quite significant changes in the bands related to C–H2 bend., summation (CH bend. + CH wagg.), and O–H vibrations of the CLA anion.

The experimental IR, RS, and INS spectra were compared with DFT, calculated for periodic boundary conditions using the CASTEP code; a good agreement was consequently achieved. The N–CH3 groups perform fast (τR ≈ 10–11–10–13 s) stochastic reorientational motions with the average energy of Ea ≈ 6.7 ± 0.6 kJ mol−1. However, below the temperature of 140 K, the fast stochastic reorientations become slow enough that they are not registered by optical spectroscopy.

Author Contributions

Conceptualization, J.N.-S. and A.P.; methodology, J.N.-S., J.H., Ł.H., A.P., J.K.M. and W.S.; validation, J.N.-S., J.H., Ł.H. and A.P.; formal analysis, J.H., Ł.H. and A.P.; investigation, J.N.-S., J.H., Ł.H., A.P., J.K.M. and W.S.; writing—original draft preparation, J.N.-S., J.H., Ł.H., J.K.M. and W.S.; writing—review and editing, J.H. and Ł.H.; visualization, J.H., Ł.H., and J.K.M.; supervision, J.H.; project administration, J.N.-S., J.H., Ł.H., and A.P.; funding acquisition, J.N.-S., J.H., and Ł.H. All authors have read and agreed to the published version of the manuscript.

Funding

Infrared absorption (FT-FIR and FT-MIR) research was carried out with the equipment purchased through the financial support provided by the European Regional Development Fund, in the framework of the Polish Innovation Economy Operational Program (contract no. POIG.02.01.00-12-023/08). This research was funded by the Grant of the Polish Plenipotentiary to JINR and JINR Directorate, grant number no: 75/2020 from 03.02.2020 subject no: 04-4-1121-2015/2020 and 04-4-1133-2018/2020.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data presented in this study are available on reasonable request from the corresponding author.

Conflicts of Interest

The authors declare no conflict of interest.

Footnotes

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References

  • 1.Lane H.-Y., Huang C.-L., Wu P.-L., Liu Y.-C., Chang Y.-C., Lin P.-Y., Chen P.-W., Tsai G. Glycine Transporter I Inhibitor, N-Methylglycine (Sarcosine), Added to Clozapine for the Treatment of Schizophrenia. Biol. Psychiatry. 2006;60:645–649. doi: 10.1016/j.biopsych.2006.04.005. [DOI] [PubMed] [Google Scholar]
  • 2.Anioła M., Dega-Szafran Z., Katrusiak A., Szafran M. NH⋯O and OH⋯O Interactions of Glycine Derivatives with Squaric Acid. New J. Chem. 2014;38:3556–3568. doi: 10.1039/C4NJ00212A. [DOI] [Google Scholar]
  • 3.Hildre A.S., Solvang S.-E.H., Aarsland D., Midtun Ø., McCann A., Ervik A.O., Nygård O., Ueland P.M., Nordrehaug J.E., Giil L.M. Components of the Choline Oxidation Pathway Modify the Association between the Apolipoprotein Ε4 Gene Variant and Cognitive Decline in Patients with Dementia. Brain Res. 2020;1726:146519. doi: 10.1016/j.brainres.2019.146519. [DOI] [PubMed] [Google Scholar]
  • 4.Graber C.D., Goust J.M., Glassman A.D., Kendall R., Loadholt C.B. Immunomodulating Properties of Dimethylglycine in Humans. J. Infect Dis. 1981;143:101–105. doi: 10.1093/infdis/143.1.101. [DOI] [PubMed] [Google Scholar]
  • 5.Cools A., Maes D., Buyse J., Kalmar I.D., Vandermeiren J.-A., Janssens G.P.J. Effect of N,N-Dimethylglycine Supplementation in Parturition Feed for Sows on Metabolism, Nutrient Digestibility and Reproductive Performance. Animal. 2010;4:2004–2011. doi: 10.1017/S1751731110001242. [DOI] [PubMed] [Google Scholar]
  • 6.Gillies N.A., Milan A.M., Chia P.H.P., Sharma P., Mitchell S.M., Zeng N., Ramzan F., D’Souza R.F., Mitchell C.J., Knowles S.O., et al. Responsiveness of One-Carbon Metabolites to a High-Protein Diet in Older Men: Results from a 10-Wk Randomized Controlled Trial. Nutrition. 2021;89:111231. doi: 10.1016/j.nut.2021.111231. [DOI] [PubMed] [Google Scholar]
  • 7.Petrosyan A.M., Ghazaryan V.V., Giester G., Fleck M., Tylczyński Z., Wiesner M. Halogenides of Dimethylglycine in Comparison with Respective Salts of Glycine, Sarcosine and Betaine. J. Mol. Struct. 2018;1158:106–121. doi: 10.1016/j.molstruc.2018.01.017. [DOI] [Google Scholar]
  • 8.Fleck M., Petrosyan A.M. Salts of Amino Acids. Springer International Publishing; Cham, Switzerland: 2014. [Google Scholar]
  • 9.Schaack G. Experimental Results on Phase Transitions in Betaine Compounds. Ferroelectrics. 1990;104:147–158. doi: 10.1080/00150199008223819. [DOI] [Google Scholar]
  • 10.Abdinejad T., Zamanloo M.R., Esrafili M.D., Seifzadeh D. Constructing a Dual-Mode Photochromic and Intrinsically Electrochromic Device Based on Organic Salts Prepared by Acid-Base Neutralization of Pyromellitic Diimides Bearing a Carboxyl Group with Aliphatic Amines. J. Photochem. Photobiol. A Chem. 2020;388:112162. doi: 10.1016/j.jphotochem.2019.112162. [DOI] [Google Scholar]
  • 11.Balashova E.V., Krichevtsov B.B., Zaitseva N.V., Yurko E.I., Svinarev F.B. Ferroelectric Thin Films of Deuterated Betaine Arsenate. Ferroelectrics. 2015;482:60–69. doi: 10.1080/00150193.2015.1056707. [DOI] [Google Scholar]
  • 12.Clarke H.T., Gillespie H.B., Weisshaus S.Z. The Action of Formaldehyde on Amines and Amino Acids1. J. Am. Chem. Soc. 1933;55:4571–4587. doi: 10.1021/ja01338a041. [DOI] [Google Scholar]
  • 13.Rodrigues V.H., Paixão J.A., Costa M.M., Beja A.M. Conformation of Cationic N,N-Dimethylglycine in Dimethylglycinium Trifluoroacetate. Acta Crystallogr. C. 2001;57:417–420. doi: 10.1107/S0108270101000087. [DOI] [PubMed] [Google Scholar]
  • 14.Pawlukojć A., Hetmańczyk J., Nowicka-Scheibe J., Maurin J.K., Schilf W., Rozwadowski Z. Spectroscopic, Thermal and Structural Studies of New l-Leucine and d-Leucine Complexes with Chloranilic Acid. J. Mol. Struct. 2017;1133:464–471. doi: 10.1016/j.molstruc.2016.12.047. [DOI] [Google Scholar]
  • 15.Hetmańczyk J., Nowicka-Scheibe J., Maurin J.K., Pawlukojć A. Low Temperature Investigations of Dynamic Properties in L-Leucine—Chloranilic Acid Complex. Spectrochim. Acta A Mol. Biomol. Spectrosc. 2018;200:281–289. doi: 10.1016/j.saa.2018.03.070. [DOI] [PubMed] [Google Scholar]
  • 16.Zaman M.B., Tomura M., Yamashita Y. Crystal Engineering Using Anilic Acids and Dipyridyl Compounds through a New Supramolecular Synthon. J. Org. Chem. 2001;66:5987–5995. doi: 10.1021/jo001746i. [DOI] [PubMed] [Google Scholar]
  • 17.Klinman J.P., Mu D. Quinoenzymes in Biology. Annu. Rev. Biochem. 1994;63:299–344. doi: 10.1146/annurev.bi.63.070194.001503. [DOI] [PubMed] [Google Scholar]
  • 18.Gómez-Zavaglia A., Fausto R. Low-Temperature Solid-State FTIR Study of Glycine, Sarcosine and N,N-Dimethylglycine: Observation of Neutral Forms of Simple α-Amino Acids in the Solid State. Phys. Chem. Chem. Phys. 2003;5:3154–3161. doi: 10.1039/B304888H. [DOI] [Google Scholar]
  • 19.Matei A., Drichko N., Gompf B., Dressel M. Far-Infrared Spectra of Amino Acids. Chem. Phys. 2005;316:61–71. doi: 10.1016/j.chemphys.2005.04.033. [DOI] [Google Scholar]
  • 20.Wolpert M., Hellwig P. Infrared Spectra and Molar Absorption Coefficients of the 20 Alpha Amino Acids in Aqueous Solutions in the Spectral Range from 1800 to 500cm−1. Spectrochim. Acta A Mol. Biomol. Spectrosc. 2006;64:987–1001. doi: 10.1016/j.saa.2005.08.025. [DOI] [PubMed] [Google Scholar]
  • 21.Culka A., Jehlička J., Edwards H.G.M. Acquisition of Raman Spectra of Amino Acids Using Portable Instruments: Outdoor Measurements and Comparison. Spectrochim. Acta A Mol. Biomol. Spectrosc. 2010;77:978–983. doi: 10.1016/j.saa.2010.08.034. [DOI] [PubMed] [Google Scholar]
  • 22.Filho P.F.F., Jiao X., Freire P.T., Lima J.A., Jr., dos Santos A.O., Henry P.F., Yokaichiya F., Kremner E., Bordallo H.N. Structure–Property Relations in Crystalline L-Leucine Obtained from Calorimetry, X-rays, Neutron and Raman Scattering. Phys. Chem. Chem. Phys. 2011;13:6576–6583. doi: 10.1039/c0cp02278k. [DOI] [PubMed] [Google Scholar]
  • 23.Pawlukojć A., Bator G., Sobczyk L., Grech E., Nowicka-Scheibe J. Inelastic Neutron Scattering, Raman, Infrared and DFT Theoretical Studies on Chloranilic Acid. J. Phys. Org. Chem. 2003;16:709–714. doi: 10.1002/poc.633. [DOI] [Google Scholar]
  • 24.CrysAlisPro Software System, Agilent Technologies, Version 1.171.33.66; CrysAlis171. NET, Release 28-04-2010. Empirical Absorption Correction Using Spherical Harmonics, Implemented in SCALE3 ABSPACK Scaling Algorithm. [(accessed on 8 April 2021)]; Available online: https://www.rigakuxrayforum.com/
  • 25.Sheldrick G.M. A Short History of SHELX. Acta Crystallogr. A. 2008;64:112–122. doi: 10.1107/S0108767307043930. [DOI] [PubMed] [Google Scholar]
  • 26.Natkaniec I., Chudoba D., Hetmańczyk L., Kazimirov V.Y., Krawczyk J., Sashin I.L., Zalewski S. Parameters of the NERA Spectrometer for Cold and Thermal Moderators of the IBR-2 Pulsed Reactor. J. Phys. Conf. Ser. 2014;554:012002. doi: 10.1088/1742-6596/554/1/012002. [DOI] [Google Scholar]
  • 27.Parker S.F., Lennon D., Albers P.W. Vibrational Spectroscopy with Neutrons: A Review of New Directions. Appl. Spectrosc. 2011;65:1325–1341. doi: 10.1366/11-06456. [DOI] [Google Scholar]
  • 28.Mitchell P.C.H., Parker S.F., Ramirez-Cuesta A.J., Tomkinson J. Vibrational Spectroscopy with Neutrons: With Applications in Chemistry, Biology, Materials Science and Catalysis. Volume 3. World Scientific; Singapore: 2005. (Series on Neutron Techniques and Applications). [Google Scholar]
  • 29.Pawlukojć A., Sobczyk L. Application of Inelastic Neutron Scattering (INS) in Studies on Low Frequency Molecular Vibrations. Trends Appl. Spectrosc. 2004;5:117–136. [Google Scholar]
  • 30.Hohenberg P., Kohn W. Inhomogeneous Electron Gas. Phys. Rev. 1964;136:B864–B871. doi: 10.1103/PhysRev.136.B864. [DOI] [Google Scholar]
  • 31.Kohn W., Sham L.J. Self-Consistent Equations Including Exchange and Correlation Effects. Phys. Rev. 1965;140:A1133–A1138. doi: 10.1103/PhysRev.140.A1133. [DOI] [Google Scholar]
  • 32.Clark S.J., Segall M.D., Pickard C.J., Hasnip P.J., Probert M.I.J., Refson K., Payne M.C. First Principles Methods Using CASTEP. Z. Kristallogr. Cryst. Mater. 2005;220:567–570. doi: 10.1524/zkri.220.5.567.65075. [DOI] [Google Scholar]
  • 33.Pfrommer B.G., Côté M., Louie S.G., Cohen M.L. Relaxation of Crystals with the Quasi-Newton Method. J. Comput. Phys. 1997;131:233–240. doi: 10.1006/jcph.1996.5612. [DOI] [Google Scholar]
  • 34.Refson K., Tulip P.R., Clark S.J. Variational Density-Functional Perturbation Theory for Dielectrics and Lattice Dynamics. Phys. Rev. B. 2006;73:155114. doi: 10.1103/PhysRevB.73.155114. [DOI] [Google Scholar]
  • 35.Perdew J.P., Burke K., Ernzerhof M. Generalized Gradient Approximation Made Simple. Phys. Rev. Lett. 1996;77:3865–3868. doi: 10.1103/PhysRevLett.77.3865. [DOI] [PubMed] [Google Scholar]
  • 36.Tkatchenko A., Scheffler M. Accurate Molecular Van Der Waals Interactions from Ground-State Electron Density and Free-Atom Reference Data. Phys. Rev. Lett. 2009;102:073005. doi: 10.1103/PhysRevLett.102.073005. [DOI] [PubMed] [Google Scholar]
  • 37.Monkhorst H.J., Pack J.D. Special points for Brillouin-zone integrations. Phys. Rev. B. 1976;13:5188–5192. doi: 10.1103/PhysRevB.13.5188. [DOI] [Google Scholar]
  • 38.Schatschneider B., Monaco S., Tkatchenko A., Liang J.-J. Understanding the Structure and Electronic Properties of Molecular Crystals Under Pressure: Application of Dispersion Corrected DFT to Oligoacenes. J. Phys. Chem. A. 2013;117:8323–8331. doi: 10.1021/jp406573n. [DOI] [PubMed] [Google Scholar]
  • 39.Dymkowski K., Parker S.F., Fernandez-Alonso F., Mukhopadhyay S. AbINS: The Modern Software for INS Interpretation. Phys. B Condens. Matter. 2018;551:443–448. doi: 10.1016/j.physb.2018.02.034. [DOI] [Google Scholar]
  • 40.Arnold O., Bilheux J.C., Borreguero J.M., Buts A., Campbell S.I., Chapon L., Doucet M., Draper N., Ferraz Leal R., Gigg M.A., et al. Mantid—Data Analysis and Visualization Package for Neutron Scattering and μ SR Experiments. Nucl. Instrum. Methods A. 2014;764:156–166. doi: 10.1016/j.nima.2014.07.029. [DOI] [Google Scholar]
  • 41.Turner M.J., McKinnon J.J., Wolff S.K., Grimwood D.J., Spackman P.R., Jayatilaka D., Spackman M.A. CrystalExplorer17. University of Western Australia. [(accessed on 8 April 2021)];2017 Available online: https://hirshfeldsurface.net.
  • 42.Pawlukojć A., Hetmańczyk J., Hetmańczyk Ł., Nowicka-Scheibe J., Maurin J.K., Schilf W., Trzybiński D., Woźniak K. Evidence of Low Temperature Phase Transition in 2,6-Dimethylpyrazine—Picric Acid Cocrystal by Means of Temperature Dependent Investigations: X-Ray, DSC and IR. J. Mol. Struct. 2021;1228:129432. doi: 10.1016/j.molstruc.2020.129432. [DOI] [Google Scholar]
  • 43.Pawlukojć A., Sobczyk L., Prager M., Bator G., Grech E., Nowicka-Scheibe J. DFT Calculations of 2,6-Dimethylpyrazine (26DMP) and Its Complex with Chloranilic Acid (CLA): Comparison to INS, IR and Raman Vibration Spectra. J. Mol. Struct. 2008;892:261–267. doi: 10.1016/j.molstruc.2008.05.038. [DOI] [Google Scholar]
  • 44.Jmol: An Open-Source Java Viewer for Chemical Structures in 3D. [(accessed on 8 April 2021)]; Available online: http://www.jmol.org/
  • 45.Sawka-Dobrowolska W., Bator G., Sobczyk L., Grech E., Nowicka-Scheibe J., Pawlukojć A. Structure and Vibrational Spectra of 1:1 Chloranilic Acid (CLA)—Tetramethylpyrazine (TMP) Complex. Struct. Chem. 2005;16:281–286. doi: 10.1007/s11224-005-4459-4. [DOI] [Google Scholar]
  • 46.Bator G., Sawka-Dobrowolska W., Sobczyk L., Owczarek M., Pawlukojć A., Grech E., Nowicka-Scheibe J. Hydrogen Bonded NHO Chains Formed by Chloranilic Acid (CLA) with 4,4′-Di-t-Butyl-2,2′-Bipyridyl (DtBBP) in the Solid State. Chem. Phys. 2012;392:114–121. doi: 10.1016/j.chemphys.2011.10.033. [DOI] [Google Scholar]
  • 47.Carabatos-Nédelec C., Becker P. Order–Disorder and Structural Phase Transitions in Solid-State Materials by Raman Scattering Analysis. J. Raman Spectrosc. 1997;28:663–671. doi: 10.1002/(SICI)1097-4555(199709)28:9<663::AID-JRS157>3.0.CO;2-L. [DOI] [Google Scholar]
  • 48.da R. Andrade P., Rao A.D.P., Katiyar R.S., Porto S.P.S. Analysis of the Relationship between Temperature Dependence of the Libration Mode and Dielectric Relaxation in NaNO2. Solid State Commun. 1973;12:847–851. doi: 10.1016/0038-1098(73)90092-6. [DOI] [Google Scholar]
  • 49.da R. Andrade P., Porto S.P.S. On Linewidth of Phonons Associated to a Disorder Mechanism. Solid State Commun. 1973;13:1249–1254. doi: 10.1016/0038-1098(73)90574-7. [DOI] [Google Scholar]

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Data Availability Statement

The data presented in this study are available on reasonable request from the corresponding author.


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