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. 2021 Jun 14;13(6):878. doi: 10.3390/pharmaceutics13060878

Figure 1.

Figure 1

Figure 1

Overview of the experimental setup and PF14 delivery of Cas9 RNP to reporter cells. (A) Outline of the general experimental setup. 1. PF14 is complexed with Cas9 RNP and added to cells. 2. The PF14 enables RNP to escape into the cytoplasm, where the nuclear localization signal relocates the RNP to the nucleus, where it cleaves the DNA in the SL construct. 3. The cells are incubated for 72 h after transfection and then analyzed by flow cytometry. (B) Flow cytometry results from the HEK293T SL cells treated with the 200 ng Cas9 RNP complexed with PF14 in increasing MR (RNP: PF14). The complexes were formed in HBG buffer. n = 3. (C) Size and zeta potential of the HBG-formulated RNP: PF14 complexes in increasing MR, as analyzed by DLS.