Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The need for appropriate dilution of the primary rabbit antiserum. Alpha-fetoprotein (AFP) immunostaining (using the polymer method) for hepatocellular carcinoma. (a): 1:400 dilution, (b): 1:3200 dilution. With 1:400 diluted antiserum, non-neoplastic hepatocytes (blue arrowheads), bile ducts (red arrowhead) and arterial wall (green arrowhead) reveal nonspecific staining. At an appropriate dilution (1:3200), positive signals of AFP are seen only in the hepatoma cells (yellow arrows).
