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Effect of prolonged storage of unstained sections (HIER-assisted ER immunostaining for breast cancer). Immunostaining just after cutting (a) and immunostaining after prolonged storage for 6 months in a −20 °C freezer (b) or at room temperature. (c). The prolonged storage at room temperature severely deteriorates the immunoreactivity of nuclear antigens such as ER. Storage in a freezer is effective for avoiding such false negativity.
