Skip to main content
. 2021 Jun 21;10(6):1560. doi: 10.3390/cells10061560

Figure 7.

Figure 7

Western blot analysis for phosphorylated and total AMPK and CAMKK2, glucose oxidation and intracellular ATP levels in SB-452533 and capsaicin treated mouse skeletal muscle cells. (a–d) Western blot and densitometric analysis of p-AMPK, AMPK, p-CAMKK2, and CAMKK2 with increasing concentrations of SB-452533 in the presence of 200 μM capsaicin. (e) Intracellular ATP level in the 0 and 200 μM capsaicin treated myotubes in the presence or absence of 200 μM SB-452533. (f) Glucose oxidation percentage in 200 μM capsaicin treated myotubes in the absence or presence of 200 μM SB-452533 and 10 nM insulin after 60 min incubation time. Data are presented as mean ± SD of three independent repeats (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 indicate a significant difference between control and SB-452533-treated groups.